C57BL/6JCya-Castor1em1flox/Cya
Common Name:
Castor1-flox
Product ID:
S-CKO-15283
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Castor1-flox
Strain ID
CKOCMP-71962-Castor1-B6J-VA
Gene Name
Product ID
S-CKO-15283
Gene Alias
2410008K03Rik; Gatsl3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Castor1em1flox/Cya mice (Catalog S-CKO-15283) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020699
NCBI RefSeq
NM_028022
Target Region
Exon 2
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Castor1, short for cytosolic arginine sensor for the mTOR complex 1 (mTORC1) subunit 1, is an important arginine sensor in the mTORC1 pathway [2]. The mTORC1 pathway is a central controller of cell proliferation in response to growth factors and nutrients, and Castor1 plays a role in this regulation, with its function having implications for various biological processes and diseases [3].
In microglia, Castor1 expression was decreased when treated with lipopolysaccharides (LPS) and interferon (IFN)-γ. Overexpression of Castor1 inhibited M1 polarization and promoted M2 polarization by inhibiting the mTOR signaling pathway, suggesting its potential as a target for central nervous system-related diseases [1]. In breast cancer, the E3 ubiquitin ligase RNF167 promotes CASTOR1 degradation, activating mTORC1 and promoting breast cancer progression [3]. In lung adenocarcinoma, Castor1 expression was reduced in tumor tissues compared to normal lung tissues. Its overexpression suppressed cellular proliferation, migration, and invasion, and low Castor1 expression was associated with a worse 5-year survival rate, indicating it might be a therapeutic target or prognostic marker [6]. In male patients with KRAS-mutated lung adenocarcinoma, elevated pCASTOR1 scores predicted poorer overall and relapse-free survival [4]. In mammary epithelial cells, heat stress increased CASTOR1 expression, and downregulation of CASTOR1 inhibited heat-stress-induced apoptosis and promoted milk component synthesis [5].
In conclusion, Castor1 is a crucial regulator in the mTORC1 pathway, with its function influencing microglial polarization, cancer progression, and responses to stress in various cell types. Studies on Castor1, including those using potential KO/CKO mouse models (although not explicitly detailed in the given references), could further our understanding of its role in central nervous system-related diseases, cancer, and other biological processes, potentially leading to new therapeutic strategies.
References:
1. Hu, Huiling, Lu, Xiaoxia, Huang, Lisi, Wang, Ying, Duan, Chaohui. 2022. Castor1 overexpression regulates microglia M1/M2 polarization via inhibiting mTOR pathway. In Metabolic brain disease, 38, 699-708. doi:10.1007/s11011-022-01135-w. https://pubmed.ncbi.nlm.nih.gov/36454504/
2. Chantranupong, Lynne, Scaria, Sonia M, Saxton, Robert A, Gygi, Steven P, Sabatini, David M. 2016. The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway. In Cell, 165, 153-164. doi:10.1016/j.cell.2016.02.035. https://pubmed.ncbi.nlm.nih.gov/26972053/
3. Li, Tingting, Wang, Xian, Ju, Enguo, Wei, Shan, Gao, Shou-Jiang. 2021. RNF167 activates mTORC1 and promotes tumorigenesis by targeting CASTOR1 for ubiquitination and degradation. In Nature communications, 12, 1055. doi:10.1038/s41467-021-21206-3. https://pubmed.ncbi.nlm.nih.gov/33594058/
4. Loo, Suet Kee, Sica, Gabriel, Wang, Xian, Stabile, Laura P, Gao, Shou-Jiang. 2024. CASTOR1 phosphorylation predicts poor survival in male patients with KRAS-mutated lung adenocarcinoma. In Cell & bioscience, 14, 127. doi:10.1186/s13578-024-01307-4. https://pubmed.ncbi.nlm.nih.gov/39385301/
5. Gai, Zhongchao, Wang, Yujiao, Wang, Jie, Zhao, Jieqiong, Gong, Guoli. 2022. Downregulation of CASTOR1 Inhibits Heat-Stress-Induced Apoptosis and Promotes Casein and Lipid Synthesis in Mammary Epithelial Cells. In Journal of agricultural and food chemistry, 70, 5386-5395. doi:10.1021/acs.jafc.2c00877. https://pubmed.ncbi.nlm.nih.gov/35442666/
6. Zhou, Xuefeng, Cheng, Zhenshun, Chen, Hao, Orang, Matthew, Zhao, Jinping. 2018. CASTOR1 suppresses the progression of lung adenocarcinoma and predicts poor prognosis. In Journal of cellular biochemistry, 119, 10186-10194. doi:10.1002/jcb.27360. https://pubmed.ncbi.nlm.nih.gov/30132978/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen