C57BL/6JCya-Prmt3em1flox/Cya
Common Name:
Prmt3-flox
Product ID:
S-CKO-15291
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Prmt3-flox
Strain ID
CKOCMP-71974-Prmt3-B6J-VA
Gene Name
Product ID
S-CKO-15291
Gene Alias
2010005E20Rik; 2410018A17Rik; Hrmt1l3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Prmt3em1flox/Cya mice (Catalog S-CKO-15291) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000032715
NCBI RefSeq
NM_133740
Target Region
Exon 3~4
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
PRMT3, a type I protein arginine methyltransferase, is crucial in biology, especially in cancer. It catalyzes the mono-methylation and asymmetric di-methylation of histone and non-histone substrates, regulating various cellular functions [5]. It is involved in multiple pathways such as retinoic acid signaling [6], glycolytic metabolism [2], and cGAS/STING signaling [4].
In various cancers, PRMT3 has been shown to play significant roles. In hepatocellular carcinoma, PRMT3-mediated arginine methylation of IGF2BP1 promotes oxaliplatin resistance, and its overexpression can be a biomarker for this resistance [1]. In glioblastoma, knockdown of PRMT3 in cell lines and patient-derived glioblastoma stem cells reduced proliferation and migration, and inhibited tumor growth in xenograft mouse models, indicating that PRMT3 promotes glioblastoma progression via enhancing HIF1A-mediated glycolysis [2]. In endometrial carcinoma, inhibition of PRMT3 enhances the susceptibility of EC cells to ferroptosis, and blocking PRMT3 improves tumor suppression in response to cisplatin and radiation therapy [3]. In hepatocellular carcinoma, hepatocyte-specific knockout of Prmt3 suppressed HCC progression, and knockout in HCC cells increased CD8+ T cell infiltration, suggesting that PRMT3 drives PD-L1-mediated immune escape through activating PDHK1-regulated glycolysis [7]. In invasive micropapillary carcinoma of the breast, overexpression of PRMT3 was an independent risk factor for poor prognosis, and treatment with a PRMT3 inhibitor decreased xenograft tumorigenic capacity [8].
In conclusion, PRMT3 is a key regulator in multiple biological processes and disease conditions, especially in various cancers. Gene knockout and conditional knockout mouse models have been instrumental in revealing its role in promoting tumor progression, treatment resistance, and immune escape in different cancer types, providing potential therapeutic targets for these diseases.
References:
1. Shi, Yunxing, Niu, Yi, Yuan, Yichuan, Yuan, Yunfei, Li, Binkui. 2023. PRMT3-mediated arginine methylation of IGF2BP1 promotes oxaliplatin resistance in liver cancer. In Nature communications, 14, 1932. doi:10.1038/s41467-023-37542-5. https://pubmed.ncbi.nlm.nih.gov/37024475/
2. Liao, Yunfei, Luo, Zaili, Lin, Yifeng, Zhou, Wenhao, Lu, Q Richard. 2022. PRMT3 drives glioblastoma progression by enhancing HIF1A and glycolytic metabolism. In Cell death & disease, 13, 943. doi:10.1038/s41419-022-05389-1. https://pubmed.ncbi.nlm.nih.gov/36351894/
3. Wang, Yiru, Wang, Can, Guan, Xue, Chen, Xiuwei, Yin, Hang. 2023. PRMT3-Mediated Arginine Methylation of METTL14 Promotes Malignant Progression and Treatment Resistance in Endometrial Carcinoma. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2303812. doi:10.1002/advs.202303812. https://pubmed.ncbi.nlm.nih.gov/37973560/
4. Shi, Yunxing, Wu, Zongfeng, Liu, Shaoru, Wang, Guocan, Li, Binkui. 2024. Targeting PRMT3 impairs methylation and oligomerization of HSP60 to boost anti-tumor immunity by activating cGAS/STING signaling. In Nature communications, 15, 7930. doi:10.1038/s41467-024-52170-3. https://pubmed.ncbi.nlm.nih.gov/39256398/
5. Huang, Jiezuo, Qiao, Beining, Yuan, Yixin, Li, Fenghe, Wang, Lei. . PRMT3 and CARM1: Emerging Epigenetic Targets in Cancer. In Journal of cellular and molecular medicine, 29, e70386. doi:10.1111/jcmm.70386. https://pubmed.ncbi.nlm.nih.gov/39964832/
6. Verma, Mamta, Khan, Mohd Imran K, Kadumuri, Rajashekar Varma, Chavali, Sreenivas, Dhayalan, Arunkumar. 2021. PRMT3 interacts with ALDH1A1 and regulates gene-expression by inhibiting retinoic acid signaling. In Communications biology, 4, 109. doi:10.1038/s42003-020-01644-3. https://pubmed.ncbi.nlm.nih.gov/33495566/
7. Ding, Chen-Hong, Yan, Fang-Zhi, Xu, Bo-Nan, Zhang, Xin, Xie, Wei-Fen. 2025. PRMT3 drives PD-L1-mediated immune escape through activating PDHK1-regulated glycolysis in hepatocellular carcinoma. In Cell death & disease, 16, 158. doi:10.1038/s41419-025-07482-7. https://pubmed.ncbi.nlm.nih.gov/40050608/
8. Zhi, Renyong, Wu, Kailiang, Zhang, Jingyue, Li, Shuai, Fu, Li. 2023. PRMT3 regulates the progression of invasive micropapillary carcinoma of the breast. In Cancer science, 114, 1912-1928. doi:10.1111/cas.15724. https://pubmed.ncbi.nlm.nih.gov/36637351/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen