C57BL/6JCya-Thumpd2em1flox/Cya
Common Name:
Thumpd2-flox
Product ID:
S-CKO-15383
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Thumpd2-flox
Strain ID
CKOCMP-72167-Thumpd2-B6J-VA
Gene Name
Product ID
S-CKO-15383
Gene Alias
2810025A12Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Thumpd2em1flox/Cya mice (Catalog S-CKO-15383) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025093
NCBI RefSeq
NM_028138
Target Region
Exon 2
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
THUMPD2, a member of the RNA-binding protein (RBP) family, is a methyltransferase that plays crucial roles in biological processes. It is involved in the N2-methylation of U6 snRNA at G72 in the spliceosome catalytic center, which is essential for efficient pre-mRNA splicing. This RNA epigenetic modification impacts global pre-mRNA splicing, influencing various physiological processes [1,3]. It is also associated with age-related macular degeneration and retinal function [1]. Additionally, THUMPD2 is part of the human TRMT112-methyltransferase network, with TRMT112 acting as a co-factor and activator for it [4].
In epithelial ovarian cancer, shRNA-mediated knockdown of THUMPD2 in OVCAR3 and SKOV3 cells increased cell proliferation but inhibited metastasis, while overexpression had the opposite effect. This indicates its dual function in promoting tumour metastasis in the late stage and inhibiting proliferation in the early stage [2]. In in vitro human esophageal squamous cell carcinoma cells, down-regulation of THUMPD2 was suggested to play a role in multidrug resistance to cisplatin and 5-fluorouracil [5].
In conclusion, THUMPD2 is significant for pre-mRNA splicing and has implications in diseases such as age-related macular degeneration, epithelial ovarian cancer, and esophageal squamous cell carcinoma. Studies on THUMPD2, including those using loss-of-function models in cell lines, help uncover its role in these biological processes and disease conditions, providing potential therapeutic targets.
References:
1. Yang, Wen-Qing, Ge, Jian-Yang, Zhang, Xiaofeng, Xu, Beisi, Liu, Ru-Juan. . THUMPD2 catalyzes the N2-methylation of U6 snRNA of the spliceosome catalytic center and regulates pre-mRNA splicing and retinal degeneration. In Nucleic acids research, 52, 3291-3309. doi:10.1093/nar/gkad1243. https://pubmed.ncbi.nlm.nih.gov/38165050/
2. Hua, Minhui, Chen, Yujie, Jia, Meiqun, Xu, Yunzhao, Zhang, Yuquan. 2024. RNA-binding protein THUMPD2 inhibits proliferation and promotes metastasis in epithelial ovarian cancer. In Heliyon, 10, e33201. doi:10.1016/j.heliyon.2024.e33201. https://pubmed.ncbi.nlm.nih.gov/39071668/
3. Wang, Can, Ulryck, Nathalie, Herzel, Lydia, Bohnsack, Katherine E, Graille, Marc. . N 2-methylguanosine modifications on human tRNAs and snRNA U6 are important for cell proliferation, protein translation and pre-mRNA splicing. In Nucleic acids research, 51, 7496-7519. doi:10.1093/nar/gkad487. https://pubmed.ncbi.nlm.nih.gov/37283053/
4. Brūmele, Baiba, Mutso, Margit, Telanne, Lilian, Abroi, Aare, Kurg, Reet. 2021. Human TRMT112-Methyltransferase Network Consists of Seven Partners Interacting with a Common Co-Factor. In International journal of molecular sciences, 22, . doi:10.3390/ijms222413593. https://pubmed.ncbi.nlm.nih.gov/34948388/
5. Hayashi, Masato, Kawakubo, Hirofumi, Fukuda, Kazumasa, Wada, Norihito, Kitagawa, Yuko. 2019. THUMP domain containing 2 protein possibly induces resistance to cisplatin and 5-fluorouracil in in vitro human esophageal squamous cell carcinoma cells as revealed by transposon activation mutagenesis. In The journal of gene medicine, 21, e3135. doi:10.1002/jgm.3135. https://pubmed.ncbi.nlm.nih.gov/31656051/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen