C57BL/6JCya-Ccpg1em1flox/Cya
Common Name:
Ccpg1-flox
Product ID:
S-CKO-15416
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ccpg1-flox
Strain ID
CKOCMP-72278-Ccpg1-B6J-VA
Gene Name
Product ID
S-CKO-15416
Gene Alias
1700030B06Rik; 1810073J13Rik; 9430028F23Rik; CPR8; D9Ertd392e
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ccpg1em1flox/Cya mice (Catalog S-CKO-15416) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000085350
NCBI RefSeq
NM_001286544
Target Region
Exon 3~5
Size of Effective Region
~3.6 kb
Detailed Document
Overview of Gene Research
Ccpg1, also known as cell cycle progression 1, is a non-canonical autophagy cargo receptor. It is essential for reticulophagy (the selective autophagy of the endoplasmic reticulum, ER-phagy), which plays a crucial role in maintaining ER proteostasis. Ccpg1 can bind to Atg8-family proteins and RB1CC1/FIP200, facilitating its function as a cargo receptor. The plasticity of autophagy, in which Ccpg1 is involved, has implications for understanding cellular metabolism and homeostasis [2,3,4,5].
In a study on corneal Aspergillus fumigatus infection, the expression of Ccpg1 in human corneal epithelial cells (HCECs) and human myeloid leukemia mononuclear cells (THP-1) macrophages increased in a time-dependent manner after infection. Its up-regulation in response to the infection was independent of dectin-1, and it colocalized with C-type lectin-like receptor-1 (CLEC-1) in THP-1 macrophages, indicating its involvement in the corneal antifungal immune response [1]. In pancreatic acinar cells, in vivo studies have shown that Ccpg1 prevents the hyper-accumulation of insoluble protein within the ER lumen, alleviates ER stress, and its loss sensitizes the exocrine pancreas to tissue injury. Ccpg1 transcripts are inducible by ER stress, directly linking ER stress to reticulophagy [4,5].
In conclusion, Ccpg1 is vital for maintaining ER proteostasis through its role in reticulophagy. Its function has been demonstrated in both corneal antifungal immune response and pancreatic ER stress management. The in vivo studies, especially those using models where Ccpg1 function is disrupted, have provided valuable insights into its role in these specific biological processes and potential implications for related disease conditions.
References:
1. Wang, Li-Mei, Chen, Xiao-Meng, Yan, Hai-Jing, Lu, Dan-Li, Che, Cheng-Ye. 2022. CCPG1 involved in corneal Aspergillus fumigatus infection. In International journal of ophthalmology, 15, 541-546. doi:10.18240/ijo.2022.04.03. https://pubmed.ncbi.nlm.nih.gov/35450172/
2. Ishii, Shunsuke, Chino, Haruka, Ode, Koji L, Mizushima, Noboru, Itakura, Eisuke. 2023. CCPG1 recognizes endoplasmic reticulum luminal proteins for selective ER-phagy. In Molecular biology of the cell, 34, ar29. doi:10.1091/mbc.E22-09-0432. https://pubmed.ncbi.nlm.nih.gov/36735498/
3. Lahiri, Vikramjit, Klionsky, Daniel J. . CCPG1 is a noncanonical autophagy cargo receptor essential for reticulophagy and pancreatic ER proteostasis. In Autophagy, 14, 1107-1109. doi:10.1080/15548627.2018.1467199. https://pubmed.ncbi.nlm.nih.gov/29911925/
4. Smith, Matthew D, Wilkinson, Simon. 2018. CCPG1, a cargo receptor required for reticulophagy and endoplasmic reticulum proteostasis. In Autophagy, 14, 1090-1091. doi:10.1080/15548627.2018.1441473. https://pubmed.ncbi.nlm.nih.gov/29916296/
5. Smith, Matthew D, Harley, Margaret E, Kemp, Alain J, Behrends, Christian, Wilkinson, Simon. 2017. CCPG1 Is a Non-canonical Autophagy Cargo Receptor Essential for ER-Phagy and Pancreatic ER Proteostasis. In Developmental cell, 44, 217-232.e11. doi:10.1016/j.devcel.2017.11.024. https://pubmed.ncbi.nlm.nih.gov/29290589/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen