Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Mir129-2em1flox/Cya
Common Name:
Mir129-2-flox
Product ID:
S-CKO-15462
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Mir129-2-flox
Strain ID
CKOCMP-723953-Mir129-2-B6J-VA
Gene Name
Mir129-2
Product ID
S-CKO-15462
Gene Alias
Mirn129-2; mir-129-2
Background
C57BL/6JCya
NCBI ID
723953
Modification
Conditional knockout
Chromosome
2
Phenotype
MGI:3618711
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mir129-2em1flox/Cya mice (Catalog S-CKO-15462) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000083577
NCBI RefSeq
NR_029752
Target Region
Exon 1
Size of Effective Region
~1.1 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Mir129-2 is a microRNA that has been shown to play important roles in various biological processes and diseases. It has been associated with regulating gene expression, and its dysregulation is linked to cancer development [1,2,3,6,7,8,9,10]. It targets genes such as SOX4, and is involved in pathways related to cell survival, migration, and angiogenesis, potentially through its influence on genes like NG2/CSPG4 [2,4]. In pancreatic beta-cell regulation, miR-129-2 can control the fate of beta-cell precursors by regulating TMEM219 expression [5].

In cancer research, studies on human cancer tissues have revealed significant findings. In gastric cancer, the MIR129-2 gene was hypermethylated in tumoral tissues compared to normal tissues, suggesting its involvement in gastric cancer development [1]. In colorectal cancer, hypermethylation of MIR129-2 promoter was associated with upregulated SOX4 mRNA expression and metastasis [2]. In hematological malignancies, MIR129-2 was frequently methylated in lymphoid malignancies, leading to its silencing, and in chronic lymphocytic leukemia (CLL), its methylation was associated with inferior survival [3]. It has also been investigated in esophageal, prostate, non-small cell lung, and breast cancers, with methylation changes of MIR129-2 being potential biomarkers for detection and diagnosis [6,7,8,9,10].

In conclusion, Mir129-2 is a crucial microRNA involved in gene regulation with implications for various biological processes, especially in the context of cancer. The research on its methylation status in different cancers, as observed in human tissue samples, highlights its potential as a biomarker and therapeutic target, contributing to a better understanding of cancer development mechanisms.

References:
1. Alizadeh, Nazila, Asadi, Milad, Shanehbandi, Dariush, Asvadi, Touraj, Sepehri, Bita. . Evaluation of the Methylation of MIR129-2 Gene in Gastric Cancer. In Journal of gastrointestinal cancer, 51, 267-270. doi:10.1007/s12029-019-00239-4. https://pubmed.ncbi.nlm.nih.gov/31073863/
2. Rezayi Soufiani, Alireza, Dolatkhah, Roya, Raeisi, Mortaza, Mohammadi, Payam, Mehdinavaz Aghdam, Abdolreza. 2021. Hypermethylation of MIR129-2 Regulates SOX4 Transcription and Associates with Metastasis in Patients with Colorectal Cancer. In Journal of gastrointestinal cancer, 53, 718-724. doi:10.1007/s12029-021-00708-9. https://pubmed.ncbi.nlm.nih.gov/34499308/
3. Wong, Kwan-Yeung, Yim, Rita Lok-Hay, Kwong, Yok-Lam, Jin, Dong-Yan, Chim, Chor-Sang. 2013. Epigenetic inactivation of the MIR129-2 in hematological malignancies. In Journal of hematology & oncology, 6, 16. doi:10.1186/1756-8722-6-16. https://pubmed.ncbi.nlm.nih.gov/23406679/
4. Ampofo, Emmanuel, Schmitt, Beate M, Menger, Michael D, Laschke, Matthias W. 2017. The regulatory mechanisms of NG2/CSPG4 expression. In Cellular & molecular biology letters, 22, 4. doi:10.1186/s11658-017-0035-3. https://pubmed.ncbi.nlm.nih.gov/28536635/
5. D'Addio, Francesca, Assi, Emma, Maestroni, Anna, Ben Nasr, Moufida, Fiorina, Paolo. 2024. TMEM219 regulates the transcription factor expression and proliferation of beta cells. In Frontiers in endocrinology, 15, 1306127. doi:10.3389/fendo.2024.1306127. https://pubmed.ncbi.nlm.nih.gov/38318298/
6. Macedo-Silva, Catarina, Constâncio, Vera, Miranda-Gonçalves, Vera, Henrique, Rui, Jerónimo, Carmen. 2023. DNA methylation biomarkers accurately detect esophageal cancer prior and post neoadjuvant chemoradiation. In Cancer medicine, 12, 8777-8788. doi:10.1002/cam4.5623. https://pubmed.ncbi.nlm.nih.gov/36670548/
7. Friedemann, Markus, Jandeck, Carsten, Tautz, Lars, Fuessel, Susanne, Menschikowski, Mario. 2024. Blood-Based DNA Methylation Analysis by Multiplexed OBBPA-ddPCR to Verify Indications for Prostate Biopsies in Suspected Prostate Cancer Patients. In Cancers, 16, . doi:10.3390/cancers16071324. https://pubmed.ncbi.nlm.nih.gov/38611002/
8. Gubenko, M S, Loginov, V I, Burdennyy, A M, Kazubskaya, T P, Pertsov, S S. 2023. Changes in the Level of Methylation of a Group of microRNA Genes as a Factor in the Development and Progression of Non-Small Cell Lung Cancer. In Bulletin of experimental biology and medicine, 174, 254-258. doi:10.1007/s10517-023-05684-7. https://pubmed.ncbi.nlm.nih.gov/36598670/
9. Burdennyy, A M, Filippova, E A, Khodyrev, D S, Loginov, V I, Braga, E A. 2021. Optimized Marker System for Early Diagnosis of Breast Cancer. In Bulletin of experimental biology and medicine, 172, 57-62. doi:10.1007/s10517-021-05331-z. https://pubmed.ncbi.nlm.nih.gov/34791555/
10. Filippova, E A, Pronina, I V, Lukina, S S, Burdennyi, A M, Loginov, V I. 2021. Relationship of the Levels of microRNA Gene Methylation with the Level of Their Expression and Pathomorphological Characteristics of Breast Cancer. In Bulletin of experimental biology and medicine, 171, 764-769. doi:10.1007/s10517-021-05312-2. https://pubmed.ncbi.nlm.nih.gov/34705180/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest