C57BL/6JCya-Mir129-2em1flox/Cya
Common Name:
Mir129-2-flox
Product ID:
S-CKO-15462
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mir129-2-flox
Strain ID
CKOCMP-723953-Mir129-2-B6J-VA
Gene Name
Product ID
S-CKO-15462
Gene Alias
Mirn129-2; mir-129-2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mir129-2em1flox/Cya mice (Catalog S-CKO-15462) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000083577
NCBI RefSeq
NR_029752
Target Region
Exon 1
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Mir129-2 is a microRNA that has been shown to play important roles in various biological processes and diseases. It has been associated with regulating gene expression, and its dysregulation is linked to cancer development [1,2,3,6,7,8,9,10]. It targets genes such as SOX4, and is involved in pathways related to cell survival, migration, and angiogenesis, potentially through its influence on genes like NG2/CSPG4 [2,4]. In pancreatic beta-cell regulation, miR-129-2 can control the fate of beta-cell precursors by regulating TMEM219 expression [5].
In cancer research, studies on human cancer tissues have revealed significant findings. In gastric cancer, the MIR129-2 gene was hypermethylated in tumoral tissues compared to normal tissues, suggesting its involvement in gastric cancer development [1]. In colorectal cancer, hypermethylation of MIR129-2 promoter was associated with upregulated SOX4 mRNA expression and metastasis [2]. In hematological malignancies, MIR129-2 was frequently methylated in lymphoid malignancies, leading to its silencing, and in chronic lymphocytic leukemia (CLL), its methylation was associated with inferior survival [3]. It has also been investigated in esophageal, prostate, non-small cell lung, and breast cancers, with methylation changes of MIR129-2 being potential biomarkers for detection and diagnosis [6,7,8,9,10].
In conclusion, Mir129-2 is a crucial microRNA involved in gene regulation with implications for various biological processes, especially in the context of cancer. The research on its methylation status in different cancers, as observed in human tissue samples, highlights its potential as a biomarker and therapeutic target, contributing to a better understanding of cancer development mechanisms.
References:
1. Alizadeh, Nazila, Asadi, Milad, Shanehbandi, Dariush, Asvadi, Touraj, Sepehri, Bita. . Evaluation of the Methylation of MIR129-2 Gene in Gastric Cancer. In Journal of gastrointestinal cancer, 51, 267-270. doi:10.1007/s12029-019-00239-4. https://pubmed.ncbi.nlm.nih.gov/31073863/
2. Rezayi Soufiani, Alireza, Dolatkhah, Roya, Raeisi, Mortaza, Mohammadi, Payam, Mehdinavaz Aghdam, Abdolreza. 2021. Hypermethylation of MIR129-2 Regulates SOX4 Transcription and Associates with Metastasis in Patients with Colorectal Cancer. In Journal of gastrointestinal cancer, 53, 718-724. doi:10.1007/s12029-021-00708-9. https://pubmed.ncbi.nlm.nih.gov/34499308/
3. Wong, Kwan-Yeung, Yim, Rita Lok-Hay, Kwong, Yok-Lam, Jin, Dong-Yan, Chim, Chor-Sang. 2013. Epigenetic inactivation of the MIR129-2 in hematological malignancies. In Journal of hematology & oncology, 6, 16. doi:10.1186/1756-8722-6-16. https://pubmed.ncbi.nlm.nih.gov/23406679/
4. Ampofo, Emmanuel, Schmitt, Beate M, Menger, Michael D, Laschke, Matthias W. 2017. The regulatory mechanisms of NG2/CSPG4 expression. In Cellular & molecular biology letters, 22, 4. doi:10.1186/s11658-017-0035-3. https://pubmed.ncbi.nlm.nih.gov/28536635/
5. D'Addio, Francesca, Assi, Emma, Maestroni, Anna, Ben Nasr, Moufida, Fiorina, Paolo. 2024. TMEM219 regulates the transcription factor expression and proliferation of beta cells. In Frontiers in endocrinology, 15, 1306127. doi:10.3389/fendo.2024.1306127. https://pubmed.ncbi.nlm.nih.gov/38318298/
6. Macedo-Silva, Catarina, Constâncio, Vera, Miranda-Gonçalves, Vera, Henrique, Rui, Jerónimo, Carmen. 2023. DNA methylation biomarkers accurately detect esophageal cancer prior and post neoadjuvant chemoradiation. In Cancer medicine, 12, 8777-8788. doi:10.1002/cam4.5623. https://pubmed.ncbi.nlm.nih.gov/36670548/
7. Friedemann, Markus, Jandeck, Carsten, Tautz, Lars, Fuessel, Susanne, Menschikowski, Mario. 2024. Blood-Based DNA Methylation Analysis by Multiplexed OBBPA-ddPCR to Verify Indications for Prostate Biopsies in Suspected Prostate Cancer Patients. In Cancers, 16, . doi:10.3390/cancers16071324. https://pubmed.ncbi.nlm.nih.gov/38611002/
8. Gubenko, M S, Loginov, V I, Burdennyy, A M, Kazubskaya, T P, Pertsov, S S. 2023. Changes in the Level of Methylation of a Group of microRNA Genes as a Factor in the Development and Progression of Non-Small Cell Lung Cancer. In Bulletin of experimental biology and medicine, 174, 254-258. doi:10.1007/s10517-023-05684-7. https://pubmed.ncbi.nlm.nih.gov/36598670/
9. Burdennyy, A M, Filippova, E A, Khodyrev, D S, Loginov, V I, Braga, E A. 2021. Optimized Marker System for Early Diagnosis of Breast Cancer. In Bulletin of experimental biology and medicine, 172, 57-62. doi:10.1007/s10517-021-05331-z. https://pubmed.ncbi.nlm.nih.gov/34791555/
10. Filippova, E A, Pronina, I V, Lukina, S S, Burdennyi, A M, Loginov, V I. 2021. Relationship of the Levels of microRNA Gene Methylation with the Level of Their Expression and Pathomorphological Characteristics of Breast Cancer. In Bulletin of experimental biology and medicine, 171, 764-769. doi:10.1007/s10517-021-05312-2. https://pubmed.ncbi.nlm.nih.gov/34705180/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen