C57BL/6NCya-Lrpprcem1flox/Cya
Common Name:
Lrpprc-flox
Product ID:
S-CKO-15469
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Lrpprc-flox
Strain ID
CKOCMP-72416-Lrpprc-B6N-VA
Gene Name
Product ID
S-CKO-15469
Gene Alias
3110001K13Rik; Gp130; Lrp130; Lsfc
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Lrpprcem1flox/Cya mice (Catalog S-CKO-15469) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000112308
NCBI RefSeq
NM_028233
Target Region
Exon 3~5
Size of Effective Region
~3.0 kb
Detailed Document
Overview of Gene Research
Lrpprc, or leucine-rich pentatricopeptide repeat-containing protein, is a member of the pentatricopeptide repeat (PPR) family. It binds to DNA, RNA, and proteins to regulate transcription and translation, and is involved in multiple biological processes such as energy metabolism, maturation and export of nuclear mRNA [2,6]. It also plays a role in maintaining normal mitochondrial translation in mammals, as it forms a complex with SLIRP in the mitochondrial matrix, which is essential for the life cycle of most mitochondrial mRNAs [5].
In disease-related studies, knockdown of Lrpprc in RAW 264.7 cells decreased foam cell formation in atherosclerosis, suggesting that Lrpprc promotes foam cell formation by inhibiting autophagy [4]. In lung cancer, Lrpprc forms a feedback loop with CDK6, promoting resistance to CDK4/6 inhibition. Gossypol acetate, a degrader of Lrpprc, enhances the sensitivity to CDK4/6 inhibitors [1]. In triple-negative breast cancer, Lrpprc promotes glycolysis by stabilizing LDHA mRNA, and knockdown of Lrpprc plus glutamine inhibitor induces synthetic lethality [3]. In ovarian cancer, HAPSTR1 binds to Lrpprc, suppresses its ubiquitination, and promotes tumor progression, while Lrpprc knockdown reverses these effects [7].
In conclusion, Lrpprc is a multifunctional protein involved in various biological processes and diseases. Studies using loss-of-function models, such as knockdown experiments, have revealed its roles in cancer, atherosclerosis, and other disease conditions, highlighting its potential as a biomarker and therapeutic target.
References:
1. Zhou, Wei, Wang, Wenxi, Liang, Yuxin, Tan, Weihong, Fang, Xiaohong. 2023. The RNA-binding protein LRPPRC promotes resistance to CDK4/6 inhibition in lung cancer. In Nature communications, 14, 4212. doi:10.1038/s41467-023-39854-y. https://pubmed.ncbi.nlm.nih.gov/37452037/
2. Cui, Jie, Wang, Li, Ren, Xiaoyue, Zhang, Yamin, Zhang, Hongyi. 2019. LRPPRC: A Multifunctional Protein Involved in Energy Metabolism and Human Disease. In Frontiers in physiology, 10, 595. doi:10.3389/fphys.2019.00595. https://pubmed.ncbi.nlm.nih.gov/31178748/
3. Yu, Yuanhang, Deng, Huifang, Wang, Wenwen, Chen, Jianying, Zhang, Bo. . LRPPRC promotes glycolysis by stabilising LDHA mRNA and its knockdown plus glutamine inhibitor induces synthetic lethality via m6 A modification in triple-negative breast cancer. In Clinical and translational medicine, 14, e1583. doi:10.1002/ctm2.1583. https://pubmed.ncbi.nlm.nih.gov/38372449/
4. Xu, Zhou, Li, Xinran, Ding, Zhiquan, Cao, Wangsen, Du, Ronghui. 2022. LRPPRC inhibits autophagy and promotes foam cell formation in atherosclerosis. In The FEBS journal, 289, 7545-7560. doi:10.1111/febs.16567. https://pubmed.ncbi.nlm.nih.gov/35792704/
5. Rubalcava-Gracia, Diana, Bubb, Kristina, Levander, Fredrik, Koolmeister, Camilla, Larsson, Nils-Göran. . LRPPRC and SLIRP synergize to maintain sufficient and orderly mammalian mitochondrial translation. In Nucleic acids research, 52, 11266-11282. doi:10.1093/nar/gkae662. https://pubmed.ncbi.nlm.nih.gov/39087558/
6. Ge, Yunxiao, Janson, Victor, Liu, Hui. 2024. Comprehensive review on leucine-rich pentatricopeptide repeat-containing protein (LRPPRC, PPR protein): A burgeoning target for cancer therapy. In International journal of biological macromolecules, 282, 136820. doi:10.1016/j.ijbiomac.2024.136820. https://pubmed.ncbi.nlm.nih.gov/39476900/
7. Li, Dongxiao, Wang, Min. 2024. An LRPPRC-HAPSTR1-PSMD14 interaction regulates tumor progression in ovarian cancer. In Aging, 16, 6773-6795. doi:10.18632/aging.205713. https://pubmed.ncbi.nlm.nih.gov/38643468/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen