C57BL/6JCya-Prr5lem1flox/Cya
Common Name
Prr5l-flox
Product ID
S-CKO-15475
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-72446-Prr5l-B6J-VA
When using this mouse strain in a publication, please cite “Prr5l-flox Mouse (Catalog S-CKO-15475) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Prr5l-flox
Strain ID
CKOCMP-72446-Prr5l-B6J-VA
Gene Name
Product ID
S-CKO-15475
Gene Alias
2600010E01Rik, 4833411O04Rik, Protor-2, Protor2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000171088
NCBI RefSeq
NM_001083810
Target Region
Exon 6
Size of Effective Region
~1.4 kb
Overview of Gene Research
Prr5l, also known as proline rich 5 like, is a protein-coding gene. It is a subunit of the mTORC2 complex and is involved in regulating cellular functions such as cell migration and apoptosis. The mTOR (mechanistic target of rapamycin) pathway, in which Prr5l participates, is crucial for cell growth, metabolism, and survival [1,2,3].
Lysophosphatidic acid (LPA) can lead to the degradation of PRR5L, which promotes mTORC2-mediated PKC-δ phosphorylation and cell migration downstream of Gα12, being important for fibroblast migration and pulmonary fibrosis development [1]. Also, PRR5L binds specifically to mTORC2 via Rictor and/or SIN1. Although not required for mTORC2 integrity or kinase activity, its knockdown reduces apoptosis, suggesting that mTORC2-dissociated PRR5L may promote apoptosis when mTOR is hyperactive [2].
In conclusion, Prr5l plays a significant role in regulating cell migration and apoptosis through its association with the mTORC2 complex. The findings from functional studies related to Prr5l contribute to understanding biological processes in areas such as pulmonary fibrosis development, providing insights into potential disease mechanisms and possible therapeutic targets.
References：
1. Gan, Xiaoqing, Wang, Jiyong, Wang, Chen, Simon, Melvin I, Wu, Dianqing. 2012. PRR5L degradation promotes mTORC2-mediated PKC-δ phosphorylation and cell migration downstream of Gα12. In Nature cell biology, 14, 686-96. doi:10.1038/ncb2507. https://pubmed.ncbi.nlm.nih.gov/22609986/
2. Thedieck, Kathrin, Polak, Pazit, Kim, Man Lyang, Arrieumerlou, Cécile, Hall, Michael N. 2007. PRAS40 and PRR5-like protein are new mTOR interactors that regulate apoptosis. In PloS one, 2, e1217. doi:. https://pubmed.ncbi.nlm.nih.gov/18030348/
3. Ozturk, Deniz Gulfem, Kocak, Muhammed, Akcay, Arzu, Kazan, Hilal, Gozuacik, Devrim. 2018. MITF-MIR211 axis is a novel autophagy amplifier system during cellular stress. In Autophagy, 15, 375-390. doi:10.1080/15548627.2018.1531197. https://pubmed.ncbi.nlm.nih.gov/30290719/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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