C57BL/6JCya-Rrp1bem1flox/Cya
Common Name:
Rrp1b-flox
Product ID:
S-CKO-15478
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rrp1b-flox
Strain ID
CKOCMP-72462-Rrp1b-B6J-VA
Gene Name
Product ID
S-CKO-15478
Gene Alias
2600005C20Rik; D030064A17; Kiaa0179; mKIAA0179
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rrp1bem1flox/Cya mice (Catalog S-CKO-15478) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000081339
NCBI RefSeq
NM_028244
Target Region
Exon 2
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Rrp1b, also known as ribosomal RNA processing1 homolog B, is a gene with significant implications in multiple biological processes. It is involved in ribosome biogenesis, as it targets protein phosphatase 1 (PP1) to mammalian cell nucleoli and is associated with Pre-60S ribosomal subunits, likely contributing to the modulation of ribosome biogenesis through reversible phosphorylation events [3]. Rrp1b also plays a role in alternative splicing regulation by interacting with SRSF1, and it has been linked to the regulation of gene expression related to metastasis [2].
Rrp1b has been identified as a candidate metastasis modifier gene in breast cancer. Functional gene assays showed its physical and functional interaction with metastasis modifier gene SIPA1, reducing tumor growth and metastatic potential. Ectopic expression of Rrp1B modulated various metastasis-predictive extracellular matrix (ECM) genes associated with tumor suppression. In breast cancer patients, certain genotypes and alleles of Rrp1B (such as the TT genotype and T allele of the 1307 T>C polymorphism) were associated with an increased risk of cancer development, progression, and were related to factors like obesity, advanced disease, increased tumor size, positive lymph node status, and Her2-negative receptor status [1].
In cervical cancer, specific single-nucleotide polymorphisms (SNPs) of Rrp1B were associated with clinicopathological features and survival outcomes, highlighting its potential as a prognostic marker [4].
In early gastric cancer, RRP1B was identified as a differentially expressed lncRNA, and its expression was significantly correlated with distal metastasis and tumor-node-metastasis staging, suggesting it may be a potential diagnostic biomarker [5].
In conclusion, Rrp1b is crucial for ribosome biogenesis and gene expression regulation, especially in the context of metastasis. Its role in breast, cervical, and gastric cancers, as revealed through genetic association studies, emphasizes its significance in understanding cancer progression and prognosis. These findings provide potential avenues for further research and the development of targeted therapies in these disease areas.
References:
1. Nanchari, Santhoshi Rani, Cingeetham, Anuradha, Meka, Phannibhushann, Digumarthi, Raghunadha Rao, Satti, Vishnupriya. 2014. Rrp1B gene polymorphism (1307T>C) in metastatic progression of breast cancer. In Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 36, 615-21. doi:10.1007/s13277-014-2613-6. https://pubmed.ncbi.nlm.nih.gov/25277657/
2. Lee, M, Dworkin, A M, Gildea, D, Moorhead, G B, Crawford, N P S. 2013. RRP1B is a metastasis modifier that regulates the expression of alternative mRNA isoforms through interactions with SRSF1. In Oncogene, 33, 1818-27. doi:10.1038/onc.2013.133. https://pubmed.ncbi.nlm.nih.gov/23604122/
3. Chamousset, Delphine, De Wever, Veerle, Moorhead, Greg B, Lamond, Angus I, Trinkle-Mulcahy, Laura. 2010. RRP1B targets PP1 to mammalian cell nucleoli and is associated with Pre-60S ribosomal subunits. In Molecular biology of the cell, 21, 4212-26. doi:10.1091/mbc.E10-04-0287. https://pubmed.ncbi.nlm.nih.gov/20926688/
4. Balčiūnienė, Eglė, Inčiūra, Arturas, Juozaitytė, Elona, Ugenskienė, Rasa. 2024. Impact of RRP1B Variants on the Phenotype, Progression, and Metastasis of Cervical Cancer. In Cancers, 16, . doi:10.3390/cancers16071250. https://pubmed.ncbi.nlm.nih.gov/38610928/
5. Lu, Qin, Yu, Ting, Ou, Xilong, Xie, Ting, Chen, Xia. 2017. Potential lncRNA diagnostic biomarkers for early gastric cancer. In Molecular medicine reports, 16, 9545-9552. doi:10.3892/mmr.2017.7770. https://pubmed.ncbi.nlm.nih.gov/29039538/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen