C57BL/6JCya-Bclaf1em1flox/Cya
Common Name:
Bclaf1-flox
Product ID:
S-CKO-15512
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Bclaf1-flox
Strain ID
CKOCMP-72567-Bclaf1-B6J-VA
Gene Name
Product ID
S-CKO-15512
Gene Alias
2610102K23Rik; 2700025J07Rik; 2810454G14Rik; 5730534O06Rik; Btf; mKIAA0164
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Bclaf1em1flox/Cya mice (Catalog S-CKO-15512) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000043881
NCBI RefSeq
NM_001025392
Target Region
Exon 5~6
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Bclaf1, also known as B-cell lymphoma-2-associated transcription factor 1, was originally identified as a regulator of apoptosis and transcription. It is associated with multiple biological processes such as DNA damage response, pre-mRNA splicing, T-cell activation, lung and muscle development, autophagy, ischemia-reperfusion injury, and viral infection. It is involved in pathways like NF-κB and Hif-1α, and its study via genetic models can provide insights into its functions [1].
In esophageal squamous cell carcinoma (ESCC), Bclaf1 is highly expressed, promoting glycolysis, cell proliferation, invasion, and spread through the YTHDF2-SIX1 pathway [2]. In hepatocellular carcinoma (HCC), it binds SPOP to stabilize PD-L1, facilitating tumor development and immune escape [3]. In lung cancer cells, it induces cisplatin resistance by regulating DNA damage repair and p21-mediated G1 phase arrest [4]. In colorectal cancer, circZFR stabilizes Bclaf1 to promote cancer progression [5]. In bile duct cancer, it may act as an anti-oncogene [6]. In osteoarthritis, Bclaf1 levels are increased in cartilage tissue, and its knockdown in chondrocytes inhibits cartilage degradation [7].
In conclusion, Bclaf1 plays diverse and context-dependent roles in various biological processes and diseases. Studies, including those using gene-knockout or conditional-knockout mouse models (implied by in vivo experiments), have revealed its significance in cancer development, drug resistance, immune escape, and cartilage degradation in osteoarthritis, providing potential therapeutic targets for these disease areas.
References:
1. Yu, Zongdong, Zhu, Jie, Wang, Haibiao, Li, Hong, Jin, Xiaofeng. 2021. Function of BCLAF1 in human disease. In Oncology letters, 23, 58. doi:10.3892/ol.2021.13176. https://pubmed.ncbi.nlm.nih.gov/34992690/
2. Zhang, Peipei, Zhang, Weiguang, Wang, Xiaoqing, Kang, Mingqiang, Ding, Chen. 2024. BCLAF1 drives esophageal squamous cell carcinoma progression through regulation of YTHDF2-dependent SIX1 mRNA degradation. In Cancer letters, 591, 216874. doi:10.1016/j.canlet.2024.216874. https://pubmed.ncbi.nlm.nih.gov/38636894/
3. Yu, Zongdong, Wu, Xiang, Zhu, Jie, Li, Hong, Jin, Xiaofeng. 2024. BCLAF1 binds SPOP to stabilize PD-L1 and promotes the development and immune escape of hepatocellular carcinoma. In Cellular and molecular life sciences : CMLS, 81, 82. doi:10.1007/s00018-024-05144-z. https://pubmed.ncbi.nlm.nih.gov/38340178/
4. Jiang, Tao, Liu, Bingjie, Wu, Dongping, Zhang, Feng. 2020. BCLAF1 induces cisplatin resistance in lung cancer cells. In Oncology letters, 20, 227. doi:10.3892/ol.2020.12090. https://pubmed.ncbi.nlm.nih.gov/32968449/
5. Chen, Jiaxin, Wang, Huijuan, Xu, Jianbin, Ju, Zhenyu, Song, Zhangfa. 2024. CircZFR promotes colorectal cancer progression via stabilizing BCLAF1 and regulating the miR-3127-5p/RTKN2 axis. In Science China. Life sciences, 67, 1881-1898. doi:10.1007/s11427-023-2514-y. https://pubmed.ncbi.nlm.nih.gov/38805063/
6. Zhou, Yutong, Ran, Xun, Han, Min. 2024. BCLAF1 is Expressed as a Potential Anti-oncogene in Bile Duct Cancer. In Biochemical genetics, 62, 3681-3694. doi:10.1007/s10528-023-10616-1. https://pubmed.ncbi.nlm.nih.gov/38198022/
7. Li, Song, Luo, Danni, Liang, Yulin, Zhao, Liming, Xiao, Jun. 2025. BCLAF1 Regulates Osteoarthritic Cartilage Degradation Through Interaction with LAMTOR2. In International journal of biological sciences, 21, 1666-1685. doi:10.7150/ijbs.100396. https://pubmed.ncbi.nlm.nih.gov/39990659/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen