C57BL/6JCya-Ppme1em1flox/Cya
Common Name:
Ppme1-flox
Product ID:
S-CKO-15519
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ppme1-flox
Strain ID
CKOCMP-72590-Ppme1-B6J-VA
Gene Name
Product ID
S-CKO-15519
Gene Alias
1110069N17Rik; 2700017M01Rik; PME-1; Pme1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ppme1em1flox/Cya mice (Catalog S-CKO-15519) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000032963
NCBI RefSeq
NM_028292
Target Region
Exon 2~3
Size of Effective Region
~2.9 kb
Detailed Document
Overview of Gene Research
PPME1, also known as protein phosphatase methylesterase 1, is a PP2A-specific methyl esterase. It negatively regulates PP2A through demethylation at its carboxy-terminal leucine 309 residue [4]. This regulation is involved in multiple biological processes and is associated with various signaling pathways, potentially having an impact on cell growth, apoptosis, and differentiation.
In pancreatic cancer, hsa_circ_0050102 binds to miR-218-5p, which in turn regulates the expression of PPME1. Elevated PPME1 levels, promoted by hsa_circ_0050102, accelerate pancreatic cancer development [1]. In thyroid carcinoma, HOTAIR sponges miR-761, which directly targets PPME1. HOTAIR promotes cell proliferation and inhibits apoptosis by regulating PPME1 expression [2]. In pancreatic carcinoma, DNAH17-AS1 down-regulates miR-432-5p, which normally targets PPME1, thus increasing PPME1 expression and promoting tumorigenesis [3]. In gastric and lung cancer, PPME1 gene amplification is identified, and knockdown of PPME1 in amplified cell lines inhibits cell proliferation and induces apoptosis [4]. In choriocarcinoma, IMP1 promotes cell migration and invasion, potentially through regulating RSK2 and PPME1 [6]. In skeletal muscle, inhibition of Ppme1 using ABL127 or AMZ30 affects MAP kinase signaling and attenuates muscle cell differentiation [5].
In conclusion, PPME1 is a crucial regulator in multiple biological processes and disease conditions. Studies, including those using gene-knockdown models, have revealed its significance in cancer development such as in pancreatic, thyroid, gastric, lung, and choriocarcinoma, as well as in muscle cell differentiation. Understanding PPME1 provides insights into disease mechanisms and potential therapeutic targets.
References:
1. Feng, Ningning, Jiao, Zhikai, Zhang, Yueshan, Yang, Baoming. 2022. Hsa_circ_0050102 regulates the pancreatic cancer development via miR-218-5p/PPME1 axis. In Journal of clinical laboratory analysis, 36, e24247. doi:10.1002/jcla.24247. https://pubmed.ncbi.nlm.nih.gov/35060203/
2. Guo, Runsheng, Ning, Yong, Ma, Ye, Shen, Na, Shi, Peidong. 2021. Long non-coding RNA HOTAIR/microRNA-761 sponge regulates PPME1 and further influences cell biological functions in thyroid carcinoma. In Laryngoscope investigative otolaryngology, 6, 438-445. doi:10.1002/lio2.593. https://pubmed.ncbi.nlm.nih.gov/34195365/
3. Xu, Tao, Lei, Ting, Li, Si-Qiao, Ding, Fei-Hu, Niu, Bin. . DNAH17-AS1 promotes pancreatic carcinoma by increasing PPME1 expression via inhibition of miR-432-5p. In World journal of gastroenterology, 26, 1745-1757. doi:10.3748/wjg.v26.i15.1745. https://pubmed.ncbi.nlm.nih.gov/32351291/
4. Li, Jing, Han, Sufang, Qian, Ziliang, Yu, De-Hua, Ji, Qunsheng. 2013. Genetic amplification of PPME1 in gastric and lung cancer and its potential as a novel therapeutic target. In Cancer biology & therapy, 15, 128-34. doi:10.4161/cbt.27146. https://pubmed.ncbi.nlm.nih.gov/24253382/
5. Labuzan, Sydney A, Lynch, Sarah A, Cooper, Lisa M, Waddell, David S. 2020. Inhibition of protein phosphatase methylesterase 1 dysregulates MAP kinase signaling and attenuates muscle cell differentiation. In Gene, 739, 144515. doi:10.1016/j.gene.2020.144515. https://pubmed.ncbi.nlm.nih.gov/32112987/
6. Hsieh, Yeun-Ting, Chou, Min-Min, Chen, Hong-Chen, Tseng, Jenn-Jhy. 2013. IMP1 promotes choriocarcinoma cell migration and invasion through the novel effectors RSK2 and PPME1. In Gynecologic oncology, 131, 182-90. doi:10.1016/j.ygyno.2013.07.106. https://pubmed.ncbi.nlm.nih.gov/23911878/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen