C57BL/6JCya-Car10em1flox/Cya
Common Name:
Car10-flox
Product ID:
S-CKO-15521
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Car10-flox
Strain ID
CKOCMP-72605-Car10-B6J-VA
Gene Name
Product ID
S-CKO-15521
Gene Alias
2700029L05Rik; Ca10
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Car10em1flox/Cya mice (Catalog S-CKO-15521) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000107863
NCBI RefSeq
NM_028296
Target Region
Exon 3
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Car10, also known as chromatin-associated RNA 10 or chromatin-associated RNA Intergenic 10, is a long non-coding RNA (lncRNA). LncRNAs play crucial roles in various biological processes, and Car10 is involved in multiple cancer-related pathways. It has been associated with cervical cancer, lung adenocarcinoma, melanoma, non-small cell lung cancer, and may contribute to tumorigenesis through different molecular mechanisms [1,2,3,4,5].
In cervical cancer, Car10 is significantly increased in tissues and cells. It acts as a competing endogenous RNA (ceRNA) to upregulate the expression of 3-phosphoinositide-dependent protein kinase 1 (PDPK1) by sponging miR-125b-5p, promoting the proliferation of cervical cancer cells [1].
In lung adenocarcinoma, Car10 is upregulated in tumor tissues, enhancing tumor metastasis. It induces epithelial-to-mesenchymal transition (EMT) by binding with miR-30 and miR-203, regulating the expression of SNAI1 and SNAI2, and also promotes angiogenesis by mediating nuclear LDHA to epigenetically regulate VEGFA/C [2,5].
In melanoma, Car10 is upregulated, and its knockdown suppresses cell proliferation, migration, and invasion in vitro and attenuates tumor growth in vivo. It promotes melanoma progression by suppressing miR-125b-5p to induce RAB3D expression [3].
In non-small cell lung cancer, Car10 is upregulated and promotes cell migration and invasion by modulating the miR-892a/GJB2 pathway [4].
In conclusion, Car10, as an lncRNA, plays oncogenic roles in multiple cancers, including cervical cancer, lung adenocarcinoma, melanoma, and non-small cell lung cancer. Through various ceRNA mechanisms and interactions with miRNAs and downstream target genes, it promotes cancer cell proliferation, metastasis, and angiogenesis. Functional studies using knockdown (akin to loss-of-function experiments) in cell lines and in vivo models have revealed these critical roles, providing potential molecular targets for cancer treatment.
References:
1. Hu, Tingting, Zhang, Qian, Gao, Lingxue. 2020. LncRNA CAR10 Upregulates PDPK1 to Promote Cervical Cancer Development by Sponging miR-125b-5p. In BioMed research international, 2020, 4351671. doi:10.1155/2020/4351671. https://pubmed.ncbi.nlm.nih.gov/32025520/
2. Ge, Xiaolu, Li, Gui-Yuan, Jiang, Lin, Xiang, Juanjuan, Li, Zheng. 2019. Long noncoding RNA CAR10 promotes lung adenocarcinoma metastasis via miR-203/30/SNAI axis. In Oncogene, 38, 3061-3076. doi:10.1038/s41388-018-0645-x. https://pubmed.ncbi.nlm.nih.gov/30617305/
3. Xie, Jing, Zheng, Yanyan, Xu, Xiaomin, Sun, Congcong, Lv, Mingfen. 2020. Long Noncoding RNA CAR10 Contributes to Melanoma Progression By Suppressing miR-125b-5p to Induce RAB3D Expression. In OncoTargets and therapy, 13, 6203-6211. doi:10.2147/OTT.S249736. https://pubmed.ncbi.nlm.nih.gov/32636644/
4. Li, Shanshan, Liu, Yize, Qiu, Guanzhen, Wang, Yong, Xia, Shuyue. 2021. Long Non-Coding RNA CAR10 Facilitates Non-Small Cell Lung Cancer Cell Migration and Invasion by Modulating the miR-892a/GJB2 Pathway. In Cancer management and research, 13, 1967-1979. doi:10.2147/CMAR.S287386. https://pubmed.ncbi.nlm.nih.gov/33664589/
5. Ge, Xiaolu, Du, Chao, Fang, Li, Wang, Ziyao, Li, Zheng. 2025. Long non-coding RNA CAR10 promotes angiogenesis of lung adenocarcinoma by mediating nuclear LDHA to epigenetically regulate VEGFA/C. In Communications biology, 8, 32. doi:10.1038/s42003-025-07452-x. https://pubmed.ncbi.nlm.nih.gov/39789173/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen