C57BL/6JCya-Hnrnpllem1flox/Cya
Common Name
Hnrnpll-flox
Product ID
S-CKO-15545
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-72692-Hnrnpll-B6J-VA
Status
When using this mouse strain in a publication, please cite “Hnrnpll-flox Mouse (Catalog S-CKO-15545) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Hnrnpll-flox
Strain ID
CKOCMP-72692-Hnrnpll-B6J-VA
Gene Name
Product ID
S-CKO-15545
Gene Alias
Hnrpll, 2510028H02Rik, 2810036L13Rik
Background
C57BL/6JCya
Gene Full Name
heterogeneous nuclear ribonucleoprotein L-like
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 17 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000184635
NCBI Transcript ID
NM_144802
Target Region
Exon 3
Size of Effective Region
~1.0 kb
Overview of Gene Research
Hnrnpll, or heterogeneous nuclear ribonucleoprotein L-like, is a tissue-specific RNA-binding protein. It is a key regulator in post-transcriptional gene regulation, mainly functioning in alternative splicing, RNA stability regulation, and translation regulation. It is involved in pathways related to cell differentiation, lymphocyte homeostasis, and cancer metastasis [3].
In embryonic stem (ES) cells, hnRNPLL depletion leads to sustained expression of ES cell-preferred isoforms, causing a differentiation deficiency and resulting in developmental defects and growth impairment in hnRNPLL-KO mice. This is due to its role in promoting multiple ES cell-preferred exon skipping events during the onset of ES cell differentiation, especially the alternative splicing of Bptf and Tbx3, which is crucial for pluripotency exit [1].
In pancreatic ductal adenocarcinoma (PDAC), hnRNPLL knockdown reduces the short isoform of MYOF (MYOFb) through exon 17 retention of MYOF, inhibiting metastasis, indicating its importance in early PDAC metastasis [2].
In B-cells, while Hnrnpllthu/thu mice show normal B-cell development in the bone marrow, they are deficient in generating germinal center (GC) B cells in response to in vivo immunization. The mutation disrupts hnRNPLL-mediated alternative splicing of the Ptprc gene in plasmablasts, but this does not affect B-cell development and function [4].
In colorectal cancer, knockdown of Hnrnpll enhances colon cancer cell invasion and metastasis ability, as it modulates the alternative splicing of CD44. Also, Hnrnpll promotes cell cycle progression and proliferation of colorectal cancer cells by stabilizing mRNA for DNA replication proteins [5,6].
In summary, hnRNPLL plays essential roles in cell differentiation, lymphocyte homeostasis, and cancer-related processes. Studies using hnRNPLL KO/CKO mouse models have significantly contributed to understanding its functions in embryonic development, cancer metastasis, and lymphocyte-related diseases. These models have provided insights into the underlying molecular mechanisms, highlighting hnRNPLL as a potential therapeutic target in relevant disease areas.
References：
1. Wang, Xue, Ping, Changyun, Tan, Puwen, Ma, Yanni, Huang, Yue. 2020. hnRNPLL controls pluripotency exit of embryonic stem cells by modulating alternative splicing of Tbx3 and Bptf. In The EMBO journal, 40, e104729. doi:10.15252/embj.2020104729. https://pubmed.ncbi.nlm.nih.gov/33349972/
2. Chen, Xianghan, Gong, Ruining, Wang, Lili, Yu, Qian, Ren, He. 2024. hnRNPLL regulates MYOF alternative splicing and correlates with early metastasis in pancreatic ductal adenocarcinoma. In Cancer letters, 611, 217436. doi:10.1016/j.canlet.2024.217436. https://pubmed.ncbi.nlm.nih.gov/39742990/
3. Chang, Xing. 2016. RNA-binding protein hnRNPLL as a critical regulator of lymphocyte homeostasis and differentiation. In Wiley interdisciplinary reviews. RNA, 7, 295-302. doi:10.1002/wrna.1335. https://pubmed.ncbi.nlm.nih.gov/26821996/
4. Yabas, Mehmet, Yazicioglu, Yavuz F, Hoyne, Gerard F, Goodnow, Christopher C, Enders, Anselm. 2021. Loss of hnRNPLL-dependent splicing of Ptprc has no impact on B-cell development, activation and terminal differentiation into antibody-secreting cells. In Immunology and cell biology, 99, 532-541. doi:10.1111/imcb.12433. https://pubmed.ncbi.nlm.nih.gov/33331104/
5. Sakuma, Keiichiro, Sasaki, Eiichi, Kimura, Kenya, Yatabe, Yasushi, Aoki, Masahiro. 2017. HNRNPLL, a newly identified colorectal cancer metastasis suppressor, modulates alternative splicing of CD44 during epithelial-mesenchymal transition. In Gut, 67, 1103-1111. doi:10.1136/gutjnl-2016-312927. https://pubmed.ncbi.nlm.nih.gov/28360095/
6. Sakuma, Keiichiro, Sasaki, Eiichi, Kimura, Kenya, Yatabe, Yasushi, Aoki, Masahiro. 2018. HNRNPLL stabilizes mRNA for DNA replication proteins and promotes cell cycle progression in colorectal cancer cells. In Cancer science, 109, 2458-2468. doi:10.1111/cas.13660. https://pubmed.ncbi.nlm.nih.gov/29869816/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
