C57BL/6JCya-Actrt2em1flox/Cya
Common Name:
Actrt2-flox
Product ID:
S-CKO-15677
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Actrt2-flox
Strain ID
CKOCMP-73353-Actrt2-B6J-VA
Gene Name
Product ID
S-CKO-15677
Gene Alias
1700052K15Rik; Arp-T2; Arpm2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Actrt2em1flox/Cya mice (Catalog S-CKO-15677) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000060062
NCBI RefSeq
NM_028513
Target Region
Exon 1
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
ACTRT2, the Actin Related Protein T2, is likely involved in cytoskeletal organization as it belongs to the actin-related protein family. It may participate in pathways related to spermiogenesis and sperm motility, given its association with proteins in these processes [1,2,3].
In research on obesity-induced asthenozoospermia, the expression of ACTRT2 was found to decline, which may contribute to reduced sperm motility and underlying sperm quality defects [1]. In the study of transient scrotal hyperthermia's effect on human sperm, the expression of ACTRT2, along with other sperm motility and energy metabolism-related proteins, tended to be reduced, suggesting its potential role in heat-induced reversible reduction in sperm concentration and motility [3].
In conclusion, ACTRT2 appears to play a significant role in sperm-related biological processes, especially in sperm motility. Research on its changes in conditions like obesity-induced asthenozoospermia and scrotal hyperthermia helps understand the molecular mechanisms underlying male infertility, providing potential targets for diagnosis and treatment in this disease area.
References：
1. Liu, Y, Guo, Y, Song, N, Guo, Q, Ding, Z. 2014. Proteomic pattern changes associated with obesity-induced asthenozoospermia. In Andrology, 3, 247-59. doi:10.1111/andr.289. https://pubmed.ncbi.nlm.nih.gov/25293813/
2. Zhang, Xiao-Zhen, Wei, Lin-Lin, Zhang, Xiao-Hui, Jin, Hui-Juan, Chen, Su-Ren. 2022. Loss of perinuclear theca ACTRT1 causes acrosome detachment and severe male subfertility in mice. In Development (Cambridge, England), 149, . doi:10.1242/dev.200489. https://pubmed.ncbi.nlm.nih.gov/35616329/
3. Wu, Yan-Qing, Rao, Meng, Hu, Shi-Fu, Zhu, Chang-Hong, Xia, Wei. 2020. Effect of transient scrotal hyperthermia on human sperm: an iTRAQ-based proteomic analysis. In Reproductive biology and endocrinology : RB&E, 18, 83. doi:10.1186/s12958-020-00640-w. https://pubmed.ncbi.nlm.nih.gov/32787870/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen