C57BL/6JCya-Nipsnap3aem1flox/Cya
Common Name:
Nipsnap3a-flox
Product ID:
S-CKO-15688
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Nipsnap3a-flox
Strain ID
CKOCMP-73398-Nipsnap3a-B6J-VA
Gene Name
Product ID
S-CKO-15688
Gene Alias
1700054F22Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nipsnap3aem1flox/Cya mice (Catalog S-CKO-15688) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000188045
NCBI RefSeq
NM_028529
Target Region
Exon 2
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Nipsnap3a is a gene encoding a protein that is predominantly a mitochondrial matrix protein. It is likely involved in vesicular trafficking and is located close to the 3' end of the ATP-binding cassette transporter A1 gene [4]. Nipsnap3a may play a role in multiple biological processes, and its study can help understand cellular homeostasis-related mechanisms.
Knockdown of Nipsnap3a in HeLa cells inhibited cell proliferation and migration, attenuated apoptosis induced by Actinomycin D, inhibited mitochondrial fission through reduction of DRP1-S616, and suppressed cytochrome c release in apoptosis. This highlights its critical role in coordinating cellular processes through influencing mitochondrial dynamics [1].
In addition, Nipsnap3a was identified as an androgen-responsive gene, potentially participating in processes like protein synthesis, response to oxidative stress, and apoptosis, which may be related to the molecular mechanisms of androgen-dependency in the prostate [2].
Also, it was recognized as one of the hub MitoEV-related genes in periodontitis, showing a high correlation with immune cells in the disease [3].
In summary, Nipsnap3a is essential for maintaining cellular homeostasis, especially by modulating mitochondrial dynamics. Its functions in androgen-related processes and the immune microenvironment of periodontitis suggest its potential importance in prostate-related diseases and periodontitis. The study of Nipsnap3a through gene knockdown models helps to better understand its role in these biological processes and disease conditions.
References:
1. Yan, Run, Chen, Liting, Cai, Zimu, Ruan, Yu, Han, Qi. 2024. NIPSNAP3A regulates cellular homeostasis by modulating mitochondrial dynamics. In Gene, 933, 148976. doi:10.1016/j.gene.2024.148976. https://pubmed.ncbi.nlm.nih.gov/39362349/
2. Romanuik, Tammy L, Wang, Gang, Holt, Robert A, Marra, Marco A, Sadar, Marianne D. 2009. Identification of novel androgen-responsive genes by sequencing of LongSAGE libraries. In BMC genomics, 10, 476. doi:10.1186/1471-2164-10-476. https://pubmed.ncbi.nlm.nih.gov/19832994/
3. Yang, Haoran, Zhao, Anna, Chen, Yuxiang, Zhou, Jianzhong, Li, Ziliang. 2024. Exploring the potential link between MitoEVs and the immune microenvironment of periodontitis based on machine learning and bioinformatics methods. In BMC oral health, 24, 169. doi:10.1186/s12903-024-03912-8. https://pubmed.ncbi.nlm.nih.gov/38308306/
4. Buechler, Christa, Bodzioch, Marek, Bared, Salim Maa, Dembinska-Kiec, Aldona, Schmitz, Gerd. . Expression pattern and raft association of NIPSNAP3 and NIPSNAP4, highly homologous proteins encoded by genes in close proximity to the ATP-binding cassette transporter A1. In Genomics, 83, 1116-24. doi:. https://pubmed.ncbi.nlm.nih.gov/15177564/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen