C57BL/6JCya-Dnm1lem1flox/Cya
Common Name:
Dnm1l-flox
Product ID:
S-CKO-15806
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Dnm1l-flox
Strain ID
CKOCMP-74006-Dnm1l-B6J-VA
Gene Name
Product ID
S-CKO-15806
Gene Alias
6330417M19Rik; Dlp1; Dnmlp1; Drp1; python
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dnm1lem1flox/Cya mice (Catalog S-CKO-15806) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000230980
NCBI RefSeq
NM_001276340
Target Region
Exon 3~5
Size of Effective Region
~3.9 kb
Detailed Document
Overview of Gene Research
Dnm1l, also known as Dynamin-1-like protein (DRP1), is a key dynamin-related GTPase. It plays a crucial role in mitochondrial and peroxisomal fission, a process that is essential for maintaining mitochondrial quality control, cell metabolism, and apoptosis regulation. Mitochondrial fission is a highly regulated process, and Dnm1l is at the center of the machinery that drives the scission of mitochondria [3,5].
In glioblastoma, BCL2L13 targets Dnm1L at the Ser616 site, leading to mitochondrial fission and high mitophagy flux, which in turn promotes the proliferation and invasion of GBM cells [1]. Targeting the DNM1L/DRP1-FIS1 axis inhibits high-grade glioma progression by impeding mitochondrial respiratory cristae remodeling [2]. Moreover, mutations in the Dnm1L gene, such as the R403C variant, can cause mitochondrial fission dysfunction, leading to severe neurological phenotypes like refractory status epilepticus, development regression, and brain atrophy [4].
In conclusion, Dnm1l is vital for mitochondrial fission and related cellular processes. Its dysregulation is associated with diseases, especially neurological disorders and glioma. Studies on Dnm1l, including those using gene-knockout models (implicit in understanding the impact of mutations as a form of functional loss), have provided insights into the molecular mechanisms underlying these disease conditions, potentially paving the way for new therapeutic strategies.
References:
1. Wang, Jiwei, Chen, Anbin, Xue, Zhiwei, Wang, Xinyu, Huang, Bin. 2023. BCL2L13 promotes mitophagy through DNM1L-mediated mitochondrial fission in glioblastoma. In Cell death & disease, 14, 585. doi:10.1038/s41419-023-06112-4. https://pubmed.ncbi.nlm.nih.gov/37660127/
2. Li, Xiaodong, Tie, Jingjing, Sun, Yuze, Yang, Yanling, Wang, Yayun. 2024. Targeting DNM1L/DRP1-FIS1 axis inhibits high-grade glioma progression by impeding mitochondrial respiratory cristae remodeling. In Journal of experimental & clinical cancer research : CR, 43, 273. doi:10.1186/s13046-024-03194-6. https://pubmed.ncbi.nlm.nih.gov/39350223/
3. Kleele, Tatjana, Rey, Timo, Winter, Julius, Pedrazzini, Thierry, Manley, Suliana. 2021. Distinct fission signatures predict mitochondrial degradation or biogenesis. In Nature, 593, 435-439. doi:10.1038/s41586-021-03510-6. https://pubmed.ncbi.nlm.nih.gov/33953403/
4. Pan, Z, Wu, T H, Chen, C, Yin, F, Peng, J. . [DNM1L gene variant caused encephalopathy, lethal, due to defective mitochondrial peroxisomal fission 1: three cases report and literature review]. In Zhonghua er ke za zhi = Chinese journal of pediatrics, 59, 400-406. doi:10.3760/cma.j.cn112140-20200921-00893. https://pubmed.ncbi.nlm.nih.gov/33902225/
5. Kraus, Felix, Roy, Krishnendu, Pucadyil, Thomas J, Ryan, Michael T. 2021. Function and regulation of the divisome for mitochondrial fission. In Nature, 590, 57-66. doi:10.1038/s41586-021-03214-x. https://pubmed.ncbi.nlm.nih.gov/33536648/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen