C57BL/6JCya-Phf19em1flox/Cya
Common Name:
Phf19-flox
Product ID:
S-CKO-15812
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Phf19-flox
Strain ID
CKOCMP-74016-Phf19-B6J-VA
Gene Name
Product ID
S-CKO-15812
Gene Alias
3110009G19Rik; 3321402G02Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Phf19em1flox/Cya mice (Catalog S-CKO-15812) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000028232
NCBI RefSeq
NM_028716
Target Region
Exon 4~7
Size of Effective Region
~3.7 kb
Detailed Document
Overview of Gene Research
PHF19, also known as PHD finger protein 19 or polycomb-like 3, is a crucial subunit of the Polycomb Repressive Complex 2 (PRC2) and plays a pivotal role in epigenetic regulation. It is involved in processes such as cell cycle regulation, differentiation, angiogenesis, and extracellular matrix organization [1,5]. Through its association with PRC2, PHF19 is linked to the trimethylation of histone H3 lysine 27 (H3K27me3), which is important for gene silencing and normal development [3,4,6].
In hepatocellular carcinoma (HCC), knocking out PHF19 in a MycF/F mouse model using the hydrodynamic method inhibited tumorigenesis and improved survival, demonstrating that PHF19 promotes HCC cell growth by activating the Hedgehog signaling pathway [2]. In multiple myeloma, functional knockdown of PHF19 led to decreased tumor growth in vitro and in vivo, highlighting its role in this cancer's biology [3]. In prostate cancer cells, depletion of PHF19 affected cell proliferation, invasive growth, and angiogenesis, revealing its role in balancing these processes [5].
In conclusion, PHF19 is an important epigenetic regulator involved in multiple biological processes. Model-based research, especially through gene knockout experiments in mouse models, has revealed its significant role in cancer development, particularly in hepatocellular carcinoma, multiple myeloma, and prostate cancer. Understanding PHF19's functions provides potential therapeutic targets for these diseases.
References:
1. Zhu, Zheng-Yi, Tang, Ning, Wang, Ming-Fu, Ren, Hao-Zhen, Shi, Xiao-Lei. 2022. Comprehensive Pan-Cancer Genomic Analysis Reveals PHF19 as a Carcinogenic Indicator Related to Immune Infiltration and Prognosis of Hepatocellular Carcinoma. In Frontiers in immunology, 12, 781087. doi:10.3389/fimmu.2021.781087. https://pubmed.ncbi.nlm.nih.gov/35069553/
2. Xiaoyun, Shi, Yuyuan, Zi, Jie, Xing, Yuezhen, Deng, Haiguang, Xin. 2021. PHF19 activates hedgehog signaling and promotes tumorigenesis in hepatocellular carcinoma. In Experimental cell research, 406, 112690. doi:10.1016/j.yexcr.2021.112690. https://pubmed.ncbi.nlm.nih.gov/34129846/
3. Schinke, Carolina D, Bird, Jordan T, Qu, Pingping, van Rhee, Frits, Walker, Brian A. 2021. PHF19 inhibition as a therapeutic target in multiple myeloma. In Current research in translational medicine, 69, 103290. doi:10.1016/j.retram.2021.103290. https://pubmed.ncbi.nlm.nih.gov/33894670/
4. Ghamlouch, Hussein, Boyle, Eileen M, Blaney, Patrick, Davies, Faith E, Morgan, Gareth J. 2021. Insights into high-risk multiple myeloma from an analysis of the role of PHF19 in cancer. In Journal of experimental & clinical cancer research : CR, 40, 380. doi:10.1186/s13046-021-02185-1. https://pubmed.ncbi.nlm.nih.gov/34857028/
5. Jain, Payal, Ballare, Cecilia, Blanco, Enrique, Vizan, Pedro, Di Croce, Luciano. 2020. PHF19 mediated regulation of proliferation and invasiveness in prostate cancer cells. In eLife, 9, . doi:10.7554/eLife.51373. https://pubmed.ncbi.nlm.nih.gov/32155117/
6. Ren, Zhihong, Ahn, Jeong Hyun, Liu, Hequn, Zheng, Deyou, Wang, Gang Greg. 2019. PHF19 promotes multiple myeloma tumorigenicity through PRC2 activation and broad H3K27me3 domain formation. In Blood, 134, 1176-1189. doi:10.1182/blood.2019000578. https://pubmed.ncbi.nlm.nih.gov/31383640/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen