C57BL/6JCya-Syce1em1flox/Cya
Common Name:
Syce1-flox
Product ID:
S-CKO-15835
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Syce1-flox
Strain ID
CKOCMP-74075-Syce1-B6J-VA
Gene Name
Product ID
S-CKO-15835
Gene Alias
4933406J07Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Syce1em1flox/Cya mice (Catalog S-CKO-15835) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000026553
NCBI RefSeq
NM_001143765
Target Region
Exon 5~8
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Syce1, the Synaptonemal complex central element 1, is a key component of the synaptonemal complex (SC) during meiosis [1,3,5,7,8,9]. The SC is crucial for the synapsis of homologous chromosomes, facilitating genetic exchange through crossing-over and accurate homologous chromosome segregation, thus being essential for the production of haploid germ cells and fertility [6,9].
In male mice, Syce1-null mutants lead to non-obstructive azoospermia (NOA) as spermatogenesis is arrested at the pachytene stage [3,5]. In females, Syce1-related mutations or deletions are associated with premature ovarian insufficiency (POI) [1,4,7,8,10]. For instance, in a consanguineous Chinese family, a homozygous deletion in Syce1 in two sisters with POI was found, while their parents had heterozygous deletions [10]. Also, overexpression or knockdown of Syce1 in Sertoli and Leydig cells in mice can activate or suppress steroidogenic genes involved in testosterone and dihydrotestosterone synthesis [2].
In conclusion, Syce1 is vital for meiosis in both male and female germ cells. Studies on Syce1 knockout or mutant models have revealed its critical role in spermatogenesis and oogenesis, with implications for understanding male infertility (such as NOA) and female infertility (such as POI). These models also help in exploring the regulation of steroid hormone synthesis related to reproductive functions.
References:
1. França, Monica Malheiros, Mendonca, Berenice Bilharinho. 2021. Genetics of ovarian insufficiency and defects of folliculogenesis. In Best practice & research. Clinical endocrinology & metabolism, 36, 101594. doi:10.1016/j.beem.2021.101594. https://pubmed.ncbi.nlm.nih.gov/34794894/
2. Wang, Qi, Yan, Qiu, Nan, Jinghong, Zhang, Yong, Zhao, Xingxu. 2022. Syce1 and Syce3 regulate testosterone and dihydrotestosterone synthesis via steroidogenic pathways in mouse Sertoli and Leydig cells. In The Journal of steroid biochemistry and molecular biology, 223, 106135. doi:10.1016/j.jsbmb.2022.106135. https://pubmed.ncbi.nlm.nih.gov/35697131/
3. Feng, Ke, Ge, Hengtao, Chen, Huanhuan, Guo, Haibin, Zhang, Lei. 2022. Novel exon mutation in SYCE1 gene is associated with non-obstructive azoospermia. In Journal of cellular and molecular medicine, 26, 1245-1252. doi:10.1111/jcmm.17180. https://pubmed.ncbi.nlm.nih.gov/35023261/
4. Qin, Yingying, Jiao, Xue, Simpson, Joe Leigh, Chen, Zi-Jiang. 2015. Genetics of primary ovarian insufficiency: new developments and opportunities. In Human reproduction update, 21, 787-808. doi:10.1093/humupd/dmv036. https://pubmed.ncbi.nlm.nih.gov/26243799/
5. Huang, Yuhua, Tian, Ruhui, Xu, Junwei, Li, Zheng, Yao, Chencheng. 2022. Novel copy number variations within SYCE1 caused meiotic arrest and non-obstructive azoospermia. In BMC medical genomics, 15, 137. doi:10.1186/s12920-022-01288-8. https://pubmed.ncbi.nlm.nih.gov/35718780/
6. Dunne, Orla M, Davies, Owen R. 2019. Molecular structure of human synaptonemal complex protein SYCE1. In Chromosoma, 128, 223-236. doi:10.1007/s00412-018-00688-z. https://pubmed.ncbi.nlm.nih.gov/30607510/
7. Hou, Dong, Yao, Chencheng, Xu, Bingying, Qin, Yingying, Guo, Ting. . Variations of C14ORF39 and SYCE1 Identified in Idiopathic Premature Ovarian Insufficiency and Nonobstructive Azoospermia. In The Journal of clinical endocrinology and metabolism, 107, 724-734. doi:10.1210/clinem/dgab777. https://pubmed.ncbi.nlm.nih.gov/34718620/
8. Pashaei, Mahdieh, Rahimi Bidgoli, Mohammad Masoud, Zare-Abdollahi, Davood, Fatehi, Farzad, Alavi, Afagh. 2020. The second mutation of SYCE1 gene associated with autosomal recessive nonobstructive azoospermia. In Journal of assisted reproduction and genetics, 37, 451-458. doi:10.1007/s10815-019-01660-1. https://pubmed.ncbi.nlm.nih.gov/31916078/
9. Sánchez-Sáez, Fernando, Gómez-H, Laura, Dunne, Orla M, Pendas, Alberto M, Davies, Owen R. 2020. Meiotic chromosome synapsis depends on multivalent SYCE1-SIX6OS1 interactions that are disrupted in cases of human infertility. In Science advances, 6, . doi:10.1126/sciadv.abb1660. https://pubmed.ncbi.nlm.nih.gov/32917591/
10. Zhe, Jing, Ye, Desheng, Chen, Xin, Zhang, Jun, Chen, Shiling. 2020. Consanguineous Chinese Familial Study Reveals that a Gross Deletion that Includes the SYCE1 Gene Region Is Associated with Premature Ovarian Insufficiency. In Reproductive sciences (Thousand Oaks, Calif.), 27, 461-467. doi:10.1007/s43032-019-00037-0. https://pubmed.ncbi.nlm.nih.gov/31925770/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen