C57BL/6JCya-Slc7a13em1flox/Cya
Common Name:
Slc7a13-flox
Product ID:
S-CKO-15838
Background:
C57BL/6JCya
Product Type
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Genotype
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Basic Information
Strain Name
Slc7a13-flox
Strain ID
CKOCMP-74087-Slc7a13-B6J-VA
Gene Name
Product ID
S-CKO-15838
Gene Alias
0610009O04Rik; AGT-1; AGT1; XAT2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc7a13em1flox/Cya mice (Catalog S-CKO-15838) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000035890
NCBI RefSeq
NM_028746
Target Region
Exon 3
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Slc7a13, also known as AGT1, is a membrane protein that functions as a cystine transporter. It is localized in the apical membrane of the S3 segment of renal proximal tubules, where it forms a heterodimer with rBAT/SLC3A1 [3]. This transporter is involved in the reabsorption of cystine and may play a role in preventing urinary loss of aspartate and glutamate [3].
Despite initial speculation, a study screening 17 cystinuria patients negative for SLC3A1 and SLC7A9 mutations found no evidence that SLC7A13 point mutations contribute to the etiology of cystinuria, suggesting other genetic factors are responsible for the phenotype in some patients [2]. In a study on urolithiasis, Slc7a13 was among the functional solute carriers used to identify distinct injured-proximal tubular cell types in calculi rats, indicating its potential role in kidney injury related to urolithiasis [1].
In conclusion, Slc7a13 is a significant cystine transporter in the renal proximal tubules. Although it has not been proven to be directly involved in cystinuria, research on animal models such as calculi rats has shown its potential association with kidney-related diseases like urolithiasis-mediated kidney injury, enhancing our understanding of renal cell biology and kidney disease mechanisms [1,2].
References:
1. Wang, Zhu, Deng, Qiong, Gu, Yanli, Wang, Xisheng, Liang, Hui. 2023. Integrated single-nucleus sequencing and spatial architecture analysis identified distinct injured-proximal tubular types in calculi rats. In Cell & bioscience, 13, 92. doi:10.1186/s13578-023-01041-3. https://pubmed.ncbi.nlm.nih.gov/37208718/
2. Olschok, Kathrin, Vester, Udo, Lahme, Sven, Kurth, Ingo, Eggermann, Thomas. 2018. No evidence for point mutations in the novel renal cystine transporter AGT1/SLC7A13 contributing to the etiology of cystinuria. In BMC nephrology, 19, 278. doi:10.1186/s12882-018-1080-5. https://pubmed.ncbi.nlm.nih.gov/30342472/
3. Nagamori, Shushi, Wiriyasermkul, Pattama, Guarch, Meritxell Espino, Palacín, Manuel, Kanai, Yoshikatsu. 2016. Novel cystine transporter in renal proximal tubule identified as a missing partner of cystinuria-related plasma membrane protein rBAT/SLC3A1. In Proceedings of the National Academy of Sciences of the United States of America, 113, 775-80. doi:10.1073/pnas.1519959113. https://pubmed.ncbi.nlm.nih.gov/26739563/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen