C57BL/6JCya-Tm9sf1em1flox/Cya
Common Name
Tm9sf1-flox
Product ID
S-CKO-15871
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-74140-Tm9sf1-B6J-VA
Status
When using this mouse strain in a publication, please cite “Tm9sf1-flox Mouse (Catalog S-CKO-15871) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Tm9sf1-flox
Strain ID
CKOCMP-74140-Tm9sf1-B6J-VA
Gene Name
Product ID
S-CKO-15871
Gene Alias
MP70, 1200014D02Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 14
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000122358
NCBI RefSeq
NM_028780
Target Region
Exon 3~4
Size of Effective Region
~2.2 kb
Overview of Gene Research
Tm9sf1, also known as MP70 and HMP70, is a member of the nine-transmembrane superfamily proteins. It has been implicated in various biological functions such as autophagy regulation, which is an important cellular process involved in degradation of proteins and organelles, and is associated with multiple diseases [2,3,4,5,6].
In disease-related research, Tm9sf1 knockout mouse models have provided valuable insights. In rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) models, Tm9sf1-/-mice produced fewer autoantibodies and showed reduced disease severity. TM9SF1 levels in patients' peripheral blood mononuclear cells were positively linked with autoantibody titers and pro-inflammatory cytokine levels, and could predict disease activity [1]. In acute lung injury (ALI) mouse models, Tm9sf1 knockout significantly alleviated LPS-induced ALI, with higher survival rate, improved pulmonary vascular permeability, decreased inflammatory cell infiltration, and downregulated inflammatory cytokines. TM9SF1 was a negative regulator of autophagy in this model [3]. In colorectal cancer models, Tm9sf1 knockout increased tumor numbers and size and enhanced tumor invasion, while TM9SF1 suppressed metastasis by targeting Vimentin for autophagic degradation [4].
In conclusion, Tm9sf1 plays essential roles in autophagy-related biological processes. The study of Tm9sf1 knockout mouse models has revealed its significance in autoimmune diseases like RA and SLE, as well as in inflammatory conditions such as ALI and in cancer metastasis. These findings provide potential therapeutic targets for the treatment of related diseases.
References：
1. Xiao, Juan, Zhao, Zhenwang, Zhou, Fengqiao, Zhang, Anbing, Wang, Ke. 2024. TM9SF1 expression correlates with autoimmune disease activity and regulates antibody production through mTOR-dependent autophagy. In BMC medicine, 22, 502. doi:10.1186/s12916-024-03729-w. https://pubmed.ncbi.nlm.nih.gov/39482663/
2. Wei, Long, Wang, Shi-Shuo, Huang, Zhi-Guang, Li, Sheng-Hua, Chen, Gang. . TM9SF1 promotes bladder cancer cell growth and infiltration. In World journal of clinical oncology, 15, 302-316. doi:10.5306/wjco.v15.i2.302. https://pubmed.ncbi.nlm.nih.gov/38455139/
3. Xiao, Juan, Shen, Xiaofang, Chen, Huabo, Zhai, Lihong, Mao, Chun. 2022. TM9SF1 knockdown decreases inflammation by enhancing autophagy in a mouse model of acute lung injury. In Heliyon, 8, e12092. doi:10.1016/j.heliyon.2022.e12092. https://pubmed.ncbi.nlm.nih.gov/36561687/
4. Wang, Huifen, Hu, Jia, Wang, Di, Li, Ang, Liu, Zhibo. 2025. TM9SF1 inhibits colorectal cancer metastasis by targeting Vimentin for Tollip-mediated selective autophagic degradation. In Cell death and differentiation, , . doi:10.1038/s41418-025-01498-4. https://pubmed.ncbi.nlm.nih.gov/40175707/
5. Azuma, Kotaro, Ikeda, Kazuhiro, Shiba, Sachiko, Tanaka, Shinya, Inoue, Satoshi. 2024. EBAG9-deficient mice display decreased bone mineral density with suppressed autophagy. In iScience, 27, 108871. doi:10.1016/j.isci.2024.108871. https://pubmed.ncbi.nlm.nih.gov/38313054/
6. He, Pengfei, Peng, Zhi, Luo, Ye, Shi, Taiping, Ma, Dalong. 2009. High-throughput functional screening for autophagy-related genes and identification of TM9SF1 as an autophagosome-inducing gene. In Autophagy, 5, 52-60. doi:. https://pubmed.ncbi.nlm.nih.gov/19029833/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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