C57BL/6JCya-Dtx2em1flox/Cya
Common Name:
Dtx2-flox
Product ID:
S-CKO-15911
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Dtx2-flox
Strain ID
CKOCMP-74198-Dtx2-B6J-VA
Gene Name
Product ID
S-CKO-15911
Gene Alias
2610524D08Rik; Deltex2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dtx2em1flox/Cya mice (Catalog S-CKO-15911) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000111142
NCBI RefSeq
NM_001256096
Target Region
Exon 4
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Dtx2, encoding Deltex E3 ubiquitin ligase 2, functions as an E3 ubiquitin ligase. It is involved in multiple pathways and biological processes. It has been associated with ubiquitination-related regulation, which can impact various cellular functions, and is of great importance in understanding cellular regulation and disease mechanisms. Genetic models, such as gene knockout models, can be valuable for studying its functions.
In non-small cell lung cancer (NSCLC), downregulation of Dtx2 suppressed cell growth and promoted ferroptosis, as it was found that Dtx2 binds to NCOA4, facilitating its ubiquitination and degradation, thus dampening NCOA4-driven ferritinophagy and ferroptosis [1].
In glioma, silencing of Dtx2 suppressed cell viability, colony formation, and migration and induced apoptosis, and Dtx2 could downregulate HLTF protein levels by increasing its ubiquitination [2].
In hepatocellular carcinoma, Dtx2 promoted anti-ferroptosis in Lenvatinib-resistant cells via downregulating the peroxisomal β-oxidation enzyme HSD17B4 [3].
In colorectal cancer cells, Dtx2 overexpression promoted migration and invasion through the Notch2/Akt axis [4].
In zebrafish, Dtx2 deficiency induced ependymo-radial glial cell proliferation and improved spinal cord motor function recovery by activating Notch-Rbpj signaling [5].
In conclusion, Dtx2 plays crucial roles in multiple biological processes and diseases. Through studies using gene knockout or knockdown models in different contexts, its functions in cancer development, ferroptosis regulation, and neural regeneration-related processes have been revealed. These findings contribute to understanding the underlying mechanisms of these diseases and may offer potential therapeutic targets.
References：
1. Liu, Zhuang, Liu, Chang, Fan, Caihong, Qi, Zhi, Shen, Yanna. 2024. E3 ubiquitin ligase DTX2 fosters ferroptosis resistance via suppressing NCOA4-mediated ferritinophagy in non-small cell lung cancer. In Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy, 77, 101154. doi:10.1016/j.drup.2024.101154. https://pubmed.ncbi.nlm.nih.gov/39366066/
2. Li, Ren, Chen, Yang, Yang, Biao, Zhao, Yuanli, Guo, Geng. 2024. DTX2 promotes glioma development via regulation of HLTF. In Biology direct, 19, 2. doi:10.1186/s13062-023-00447-w. https://pubmed.ncbi.nlm.nih.gov/38163902/
3. Zhang, Zhongyan, Zhou, Qi, Li, Zhenchong, Zhang, Chuanzhao, Huang, Shanzhou. 2025. DTX2 attenuates Lenvatinib-induced ferroptosis by suppressing docosahexaenoic acid biosynthesis through HSD17B4-dependent peroxisomal β-oxidation in hepatocellular carcinoma. In Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy, 81, 101224. doi:10.1016/j.drup.2025.101224. https://pubmed.ncbi.nlm.nih.gov/40058099/
4. Ma, Z, Zhao, X, Zhang, X, Xu, G, Liu, F. . [DTX2 overexpression promotes migration and invasion of colorectal cancer cells through the Notch2/Akt axis]. In Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 43, 340-348. doi:10.12122/j.issn.1673-4254.2023.03.02. https://pubmed.ncbi.nlm.nih.gov/37087577/
5. Chen, Hao-Yuan, Huang, Yin-Cheng, Yeh, Tu-Hsueh, Sun, Mu-Qun, Cheng, Yi-Chuan. 2024. Dtx2 Deficiency Induces Ependymo-Radial Glial Cell Proliferation and Improves Spinal Cord Motor Function Recovery. In Stem cells and development, 33, 540-550. doi:10.1089/scd.2023.0247. https://pubmed.ncbi.nlm.nih.gov/39001828/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen