C57BL/6JCya-Eif4enif1em1flox/Cya
Common Name:
Eif4enif1-flox
Product ID:
S-CKO-15916
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Eif4enif1-flox
Strain ID
CKOCMP-74203-Eif4enif1-B6J-VA
Gene Name
Product ID
S-CKO-15916
Gene Alias
2610509L04Rik; A930019J01Rik; Clast4; D11Ertd166e
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Eif4enif1em1flox/Cya mice (Catalog S-CKO-15916) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000110048
NCBI RefSeq
NM_001166549
Target Region
Exon 3~4
Size of Effective Region
~3.0 kb
Detailed Document
Overview of Gene Research
Eif4enif1, also known as eukaryotic translation initiation factor 4E nuclear import factor 1, encodes an eukaryotic translation initiation factor 4E transporter (4E-T) protein. It suppresses the expression of capped mRNAs, playing a role in inhibiting mRNA translation and regulating mRNA destabilization [6].
Eif4enif1 haploinsufficiency in mouse models causes subfertility, impairs oocyte maturation and partially arrests early embryonic development. Dual-omic sequencing shows it significantly alters both transcriptome and translatome in mouse oocytes, leading to oocyte mitochondrial hyperfusion and mitochondria-associated ribonucleoprotein domain distribution alteration [1]. In human studies, various Eif4enif1 variants have been identified in patients with non-syndromic premature ovarian insufficiency (POI). These variants exhibit reduced mRNA and protein expression levels, and impaired translation regulation abilities, potentially contributing to POI [2,3,4,5].
In conclusion, Eif4enif1 is crucial for normal oocyte development and ovarian function. Mouse models with Eif4enif1 haploinsufficiency have provided insights into the molecular mechanisms underlying infertility, especially those related to POI. Understanding Eif4enif1's function may offer new therapeutic targets for infertility treatment.
References：
1. Ding, Yuxi, He, Zequn, Sha, Yanwei, Kee, Kehkooi, Li, Lin. 2023. Eif4enif1 haploinsufficiency disrupts oocyte mitochondrial dynamics and leads to subfertility. In Development (Cambridge, England), 150, . doi:10.1242/dev.202151. https://pubmed.ncbi.nlm.nih.gov/38088064/
2. Shang, Lingyue, Ren, Shuting, Yang, Xi, Zhang, Xiaojin, Wu, Yanhua. 2022. EIF4ENIF1 variants in two patients with non-syndromic premature ovarian insufficiency. In European journal of medical genetics, 65, 104597. doi:10.1016/j.ejmg.2022.104597. https://pubmed.ncbi.nlm.nih.gov/36030004/
3. Ding, Yuxi, Chen, Shuya, Jin, Jing, Xin, Mingwei, Li, Lin. 2024. POI-associated EIF4ENIF1 mutations exhibit impaired translation regulation abilities. In Gene, 917, 148456. doi:10.1016/j.gene.2024.148456. https://pubmed.ncbi.nlm.nih.gov/38604507/
4. Zhao, Minying, Feng, Fan, Chu, Chunfang, Yue, Wentao, Li, Lin. 2019. A novel EIF4ENIF1 mutation associated with a diminished ovarian reserve and premature ovarian insufficiency identified by whole-exome sequencing. In Journal of ovarian research, 12, 119. doi:10.1186/s13048-019-0595-0. https://pubmed.ncbi.nlm.nih.gov/31810472/
5. Kasippillai, Thushiga, MacArthur, Daniel G, Kirby, Andrew, Daly, Mark J, Welt, Corrine K. 2013. Mutations in eIF4ENIF1 are associated with primary ovarian insufficiency. In The Journal of clinical endocrinology and metabolism, 98, E1534-9. doi:10.1210/jc.2013-1102. https://pubmed.ncbi.nlm.nih.gov/23902945/
6. Chang, Sung-Hee, Elemento, Olivier, Zhang, Jiasheng, Simons, Michael, Hla, Timothy. 2014. ELAVL1 regulates alternative splicing of eIF4E transporter to promote postnatal angiogenesis. In Proceedings of the National Academy of Sciences of the United States of America, 111, 18309-14. doi:10.1073/pnas.1412172111. https://pubmed.ncbi.nlm.nih.gov/25422430/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen