C57BL/6JCya-Liat1em1flox/Cya
Common Name:
Liat1-flox
Product ID:
S-CKO-15925
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Liat1-flox
Strain ID
CKOCMP-74230-Liat1-B6J-VA
Gene Name
Product ID
S-CKO-15925
Gene Alias
1700016K19Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Liat1em1flox/Cya mice (Catalog S-CKO-15925) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000066408
NCBI RefSeq
NM_198637
Target Region
Exon 2
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Liat1, short for Ligand of Ate1, is a protein initially discovered through its interaction with arginyl-tRNA protein transferase 1 (Ate1), a component of the Arg/N-degron pathway of protein degradation. It stimulates the in vitro N-terminal arginylation of a model substrate by Ate1 and has a higher affinity for certain Ate1 isoforms [1]. Vertebrate and some invertebrate genomes encode Liat1-like proteins. In primates, Liat1 genes are subtelomeric, and Liat1 proteins in some primates have tandem repeats of a 10-residue sequence, which may contribute to primate evolution [1].
Mouse Liat1's N-terminal half has an intrinsically disordered region (IDR) facilitating liquid-liquid phase separation (LLPS) in the nucleolus. A poly-K region within this IDR targets Liat1 to the nucleolus, and Jmjd6 modifies Liat1, inhibiting its nucleolar targeting [2]. Also, human tRNAArg can bind recombinant mouse LIAT1, suggesting LIAT1 is an RNA-binding protein, which may be related to TDP-43 proteostasis and associated diseases [3].
In conclusion, Liat1 is involved in protein degradation pathways via its interaction with Ate1, participates in nucleolar LLPS, and has the potential to bind RNA. Studies on Liat1, especially those using mouse models, contribute to understanding its role in protein and RNA metabolism, as well as its possible implications in neurodegenerative and myodegenerative diseases related to TDP-43 malfunctions [1,2,3].
References:
1. Brower, Christopher S, Rosen, Connor E, Jones, Richard H, Piatkov, Konstantin I, Varshavsky, Alexander. 2014. Liat1, an arginyltransferase-binding protein whose evolution among primates involved changes in the numbers of its 10-residue repeats. In Proceedings of the National Academy of Sciences of the United States of America, 111, E4936-45. doi:10.1073/pnas.1419587111. https://pubmed.ncbi.nlm.nih.gov/25369936/
2. Arva, Akshaya, Kasu, Yasar Arfat T, Duncan, Jennifer, Alkhatatbeh, Mosleh A, Brower, Christopher S. 2020. The Ligand of Ate1 is intrinsically disordered and participates in nucleolar phase separation regulated by Jumonji Domain Containing 6. In Proceedings of the National Academy of Sciences of the United States of America, 118, . doi:10.1073/pnas.2015887118. https://pubmed.ncbi.nlm.nih.gov/33443146/
3. Sjekloća, Ljiljana, Buratti, Emanuele. 2024. tRNA Arg binds in vitro TDP-43 RNA recognition motifs and ligand of Ate1 protein LIAT1. In microPublication biology, 2024, . doi:10.17912/micropub.biology.001224. https://pubmed.ncbi.nlm.nih.gov/39081859/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen