C57BL/6JCya-Nkx6-3em1flox/Cya
Common Name
Nkx6-3-flox
Product ID
S-CKO-16052
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-74561-Nkx6-3-B6J-VA
When using this mouse strain in a publication, please cite “Nkx6-3-flox Mouse (Catalog S-CKO-16052) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Nkx6-3-flox
Strain ID
CKOCMP-74561-Nkx6-3-B6J-VA
Gene Name
Product ID
S-CKO-16052
Gene Alias
9130417I07Rik, Nkx6.3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 8
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000071588
NCBI RefSeq
NM_029002
Target Region
Exon 2
Size of Effective Region
~1.4 kb
Overview of Gene Research
Nkx6-3, a transcription factor, is crucial for gastric mucosal epithelial differentiation. It is involved in pathways such as amyloid β (Aβ) production regulation, Wnt/β-catenin and Rho-GTPase signaling, and also impacts reactive oxygen species (ROS) production [1,3,6]. Genetic models, like gene knockout in mice, can be valuable for studying its functions.
In gastric-related studies, NKX6.3 depletion in human gastric epithelial cells led to multiple outcomes. It induced cell death, increased Aβ peptide oligomers production, and upregulated related proteins, causing gastric mucosal atrophy [1]. NKX6.3 depletion also promoted cell cycle progression, mitotic defects, genomic instability, and abnormal DNA replication and repair, contributing to gastric carcinogenesis [2,4]. Additionally, it led to abnormal expression of CDX2 and SOX2, potentially causing gastric-to-intestinal transdifferentiation, and activated AICDA/APOBEC family, resulting in genetic mutations in gastric epithelial cells [5,7].
In conclusion, Nkx6-3 is essential for maintaining gastric epithelial homeostasis, regulating mitotic integrity, genomic stability, and differentiation in the stomach. Studies using Nkx6-3 depletion models have significantly contributed to understanding its role in gastric mucosal atrophy and carcinogenesis, providing potential therapeutic targets for these gastric diseases [1-3,5-7,9].
References：
1. Yoon, Jung Hwan, Lee, Yeon Soo, Kim, Olga, Nam, Suk Woo, Park, Won Sang. . NKX6.3 protects against gastric mucosal atrophy by downregulating β-amyloid production. In World journal of gastroenterology, 25, 330-345. doi:10.3748/wjg.v25.i3.330. https://pubmed.ncbi.nlm.nih.gov/30686901/
2. Yoon, Jung Hwan, Kim, Jeong-Kyu, Eun, Jung Woo, Nam, Suk Woo, Park, Won Sang. 2025. NKX6.3 modulation of mitotic dynamics and genomic stability in gastric carcinogenesis. In Cell communication and signaling : CCS, 23, 35. doi:10.1186/s12964-025-02030-4. https://pubmed.ncbi.nlm.nih.gov/39833908/
3. Yoon, Jung Hwan, Kim, Olga, Nam, Suk Woo, Lee, Jung Young, Park, Won Sang. 2017. NKX6.3 Regulates Reactive Oxygen Species Production by Suppressing NF-kB and DNMT1 Activities in Gastric Epithelial Cells. In Scientific reports, 7, 2807. doi:10.1038/s41598-017-02901-y. https://pubmed.ncbi.nlm.nih.gov/28584243/
4. Yoon, Jung Hwan, Eun, Jung Woo, Ashktorab, Hassan, Nam, Suk Woo, Park, Won Sang. 2021. Depletion of NK6 Homeobox 3 (NKX6.3) causes gastric carcinogenesis through copy number alterations by inducing impairment of DNA replication and repair regulation. In Oncogenesis, 10, 85. doi:10.1038/s41389-021-00365-4. https://pubmed.ncbi.nlm.nih.gov/34893582/
5. Yoon, Jung H, Choi, Sung S, Kim, Olga, Lee, Jung Y, Park, Won S. 2016. Inactivation of NKX6.3 in the stomach leads to abnormal expression of CDX2 and SOX2 required for gastric-to-intestinal transdifferentiation. In Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 29, 194-208. doi:10.1038/modpathol.2015.150. https://pubmed.ncbi.nlm.nih.gov/26743476/
6. Yoon, Jung Hwan, Eun, Jung Woo, Choi, Won Suk, Lee, Jung Young, Park, Won Sang. 2016. NKX6.3 Is a Transcription Factor for Wnt/β-catenin and Rho-GTPase Signaling-Related Genes to Suppress Gastric Cancer Progression. In EBioMedicine, 9, 97-109. doi:10.1016/j.ebiom.2016.05.027. https://pubmed.ncbi.nlm.nih.gov/27333045/
7. Yoon, Jung Hwan, Kim, Olga, Eun, Jung Woo, Nam, Suk Woo, Park, Won Sang. 2018. Multiple genetic mutations caused by NKX6.3 depletion contribute to gastric tumorigenesis. In Scientific reports, 8, 17609. doi:10.1038/s41598-018-35733-5. https://pubmed.ncbi.nlm.nih.gov/30514953/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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