C57BL/6JCya-Ddit4em1flox/Cya
Common Name
Ddit4-flox
Product ID
S-CKO-16101
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-74747-Ddit4-B6J-VA
When using this mouse strain in a publication, please cite “Ddit4-flox Mouse (Catalog S-CKO-16101) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Ddit4-flox
Strain ID
CKOCMP-74747-Ddit4-B6J-VA
Gene Name
Product ID
S-CKO-16101
Gene Alias
5830413E08Rik, REDD1, Rtp801, dig2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 10
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000020308
NCBI RefSeq
NM_029083
Target Region
Exon 2~3
Size of Effective Region
~2.0 kb
Overview of Gene Research
Ddit4, also known as REDD1, is a gene that plays a crucial role in regulating the cellular energy hub mTORC1. It suppresses mTORC1 initially and then licenses its re-activation, which is important for normal cell proliferation. The mTORC1 pathway is closely associated with various cellular processes such as growth, metabolism, and autophagy [1,2,3].
In mice, Ddit4-deficient models have provided key insights. Paligenotic Ddit4 -/- chief cells in the stomach maintain constitutively high mTORC1. This leads to increased mitosis of metaplastic cells despite DNA damage, increasing the risk of tumorigenesis. MNU-treated Ddit4 -/- mice had increased spontaneous tumorigenesis after multiple rounds of paligenosis induced by TAM [1].
In conclusion, Ddit4 is essential in regulating mTORC1-related cell proliferation. Gene-knockout mouse models, like the Ddit4 -/- mice, have revealed its significance in preventing the proliferation of damaged cells and thus reducing tumor formation in the context of injury-induced metaplasia in the stomach [1].
References：
1. Miao, Zhi-Feng, Sun, Jing-Xu, Adkins-Threats, Mahliyah, Wang, Zhen-Ning, Mills, Jason C. 2020. DDIT4 Licenses Only Healthy Cells to Proliferate During Injury-induced Metaplasia. In Gastroenterology, 160, 260-271.e10. doi:10.1053/j.gastro.2020.09.016. https://pubmed.ncbi.nlm.nih.gov/32956680/
2. Jiang, Ting, Zhu, Jiaojiao, Jiang, Shilong, Zhou, Wenhu, Cheng, Yan. 2023. Targeting lncRNA DDIT4-AS1 Sensitizes Triple Negative Breast Cancer to Chemotherapy via Suppressing of Autophagy. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2207257. doi:10.1002/advs.202207257. https://pubmed.ncbi.nlm.nih.gov/37096846/
3. Zhang, Yi, Liu, Xiaomeng, Wang, Yan, Wang, Hongquan, Tang, Bo. 2022. The m6A demethylase ALKBH5-mediated upregulation of DDIT4-AS1 maintains pancreatic cancer stemness and suppresses chemosensitivity by activating the mTOR pathway. In Molecular cancer, 21, 174. doi:10.1186/s12943-022-01647-0. https://pubmed.ncbi.nlm.nih.gov/36056355/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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