C57BL/6JCya-Trmoem1flox/Cya
Common Name:
Trmo-flox
Product ID:
S-CKO-16103
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Trmo-flox
Strain ID
CKOCMP-74753-Trmo-B6J-VA
Gene Name
Product ID
S-CKO-16103
Gene Alias
5830415F09Rik; Nap1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Trmoem1flox/Cya mice (Catalog S-CKO-16103) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000086563
NCBI RefSeq
NM_029086
Target Region
Exon 2
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Trmo, also known as yaeB in Escherichia coli, is a tRNA methyltransferase. It belongs to the Class VIII of AdoMet-dependent methyltransferases and is responsible for the N6-methylation of N6-threonylcarbamoyladenosine (m6t6A) in tRNAs [2]. This modification is crucial for efficient decoding of ACY codons in tRNA(Thr), ensuring proper translation. It may also be involved in broader metabolic and signal-transduction-related pathways as it was identified among genes associated with differentiated thyroid carcinoma (DTC) [1].
In E. coli, deletion of the trmO gene led to the conversion of m6t6A to cyclic t(6)A (ct(6)A), indicating that t(6)A is a common precursor for both m6t6A and ct(6)A. Also, N6-methylation of t(6)A by TrmO enhanced the attenuation activity of the thr operon, suggesting its role in efficient decoding [2]. In silico studies on a hypothetical protein from Pasteurella multocida with a tRNA (adenine (37)-N6)-methyltransferase TrmO functional domain showed its interaction with SAM and SAH, crucial metabolites in tRNA methylation, providing evidence for its possible role as an SAM-dependent MTase [3].
In conclusion, Trmo is an important tRNA methyltransferase for proper tRNA modification and translation efficiency. Its identification in DTC-associated gene loci implies a potential role in this disease. Studies on Trmo in bacteria and in silico models contribute to understanding its biological functions and its possible implications in human diseases like DTC.
References：
1. Kulkarni, Om, Sugier, Pierre-Emmanuel, Guibon, Julie, Truong, Thérèse, Lesueur, Fabienne. 2021. Gene network and biological pathways associated with susceptibility to differentiated thyroid carcinoma. In Scientific reports, 11, 8932. doi:10.1038/s41598-021-88253-0. https://pubmed.ncbi.nlm.nih.gov/33903625/
2. Kimura, Satoshi, Miyauchi, Kenjyo, Ikeuchi, Yoshiho, Crécy-Lagard, Valérie de, Suzuki, Tsutomu. 2014. Discovery of the β-barrel-type RNA methyltransferase responsible for N6-methylation of N6-threonylcarbamoyladenosine in tRNAs. In Nucleic acids research, 42, 9350-65. doi:10.1093/nar/gku618. https://pubmed.ncbi.nlm.nih.gov/25063302/
3. Masum, Md Habib Ullah, Rajia, Sultana, Bristi, Uditi Paul, Rahaman, Md Mizanur, Saha, Otun. 2023. In Silico Functional Characterization of a Hypothetical Protein From Pasteurella Multocida Reveals a Novel S-Adenosylmethionine-Dependent Methyltransferase Activity. In Bioinformatics and biology insights, 17, 11779322231184024. doi:10.1177/11779322231184024. https://pubmed.ncbi.nlm.nih.gov/37424709/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen