C57BL/6JCya-Kbtbd11em1flox/Cya
Common Name:
Kbtbd11-flox
Product ID:
S-CKO-16131
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Kbtbd11-flox
Strain ID
CKOCMP-74901-Kbtbd11-B6J-VA
Gene Name
Product ID
S-CKO-16131
Gene Alias
2900016B01Rik; 4930465M17Rik; mKIAA0711
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Kbtbd11em1flox/Cya mice (Catalog S-CKO-16131) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000069399
NCBI RefSeq
NM_029116
Target Region
Exon 2
Size of Effective Region
~3.0 kb
Detailed Document
Overview of Gene Research
Kbtbd11, Kelch repeat and BTB domain-containing protein 11, is involved in multiple biological processes. It is associated with adipogenesis, osteoclastogenesis, and may play a role in tumorigenesis. In adipocytes, it may be part of pathways related to adipocyte differentiation and homeostasis, and in osteoclasts, it is involved in the regulation of osteoclast differentiation pathway [1,2,3,4].
In adipocyte-related functional studies, knockdown of Kbtbd11 in 3T3-L1 cells suppressed adipogenesis, while overexpression promoted it. Its mRNA levels increase during 3T3-L1 differentiation, and are affected by feeding states in mice, with higher levels in re-fed and diet-induced obese mice. Also, PPARγ acts on the Kbtbd11 gene region to regulate its expression, and it interacts with HSC70 and HSP60 to regulate NFATc1 proteolysis. In osteoclasts, knockdown of Kbtbd11 enhanced osteoclast formation and increased osteoclast marker genes expression, while overexpression impaired osteoclast differentiation. Kbtbd11 interacts with Cullin3 to promote ubiquitination and degradation of NFATc1, a key factor for osteoclast differentiation [1,2,3,4].
In conclusion, Kbtbd11 is essential for adipocyte differentiation and osteoclast regulation. Studies using gene knockdown (a form of in-vivo loss-of-function model) in cell lines like 3T3-L1 and in understanding osteoclastogenesis have revealed its role. These findings suggest its potential as a therapeutic target in obesity and perhaps in bone-related diseases where osteoclast activity needs regulation [1,2,3,4].
References:
1. Watanabe, Kazuhisa, Matsumoto, Ayumi, Tsuda, Hidetoshi, Iwamoto, Sadahiko. 2022. KBTBD11, encoding a novel PPARγ target gene, is involved in NFATc1 proteolysis by interacting with HSC70 and HSP60. In Scientific reports, 12, 20273. doi:10.1038/s41598-022-24929-5. https://pubmed.ncbi.nlm.nih.gov/36434268/
2. Watanabe, Kazuhisa, Yokota, Kazuha, Yoshida, Ken, Matsumoto, Ayumi, Iwamoto, Sadahiko. 2019. Kbtbd11 contributes to adipocyte homeostasis through the activation of upstream stimulatory factor 1. In Heliyon, 5, e02777. doi:10.1016/j.heliyon.2019.e02777. https://pubmed.ncbi.nlm.nih.gov/31844712/
3. Narahara, Shun, Sakai, Eiko, Kadowaki, Tomoko, Asahina, Izumi, Tsukuba, Takayuki. 2019. KBTBD11, a novel BTB-Kelch protein, is a negative regulator of osteoclastogenesis through controlling Cullin3-mediated ubiquitination of NFATc1. In Scientific reports, 9, 3523. doi:10.1038/s41598-019-40240-2. https://pubmed.ncbi.nlm.nih.gov/30837587/
4. Watanabe, Kazuhisa, Yoshida, Ken, Iwamoto, Sadahiko. 2019. Kbtbd11 gene expression in adipose tissue increases in response to feeding and affects adipocyte differentiation. In Journal of diabetes investigation, 10, 925-932. doi:10.1111/jdi.12995. https://pubmed.ncbi.nlm.nih.gov/30582777/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen