C57BL/6NCya-Mindy1em1flox/Cya
Common Name:
Mindy1-flox
Product ID:
S-CKO-16143
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mindy1-flox
Strain ID
CKOCMP-75007-Mindy1-B6N-VA
Gene Name
Product ID
S-CKO-16143
Gene Alias
1810005H09Rik; 4930504E06Rik; Fam63a; cI-40; mKIAA1390; ni
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Mindy1em1flox/Cya mice (Catalog S-CKO-16143) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000107187
NCBI RefSeq
NM_199475
Target Region
Exon 3~6
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
MINDY1, a member of the motif interacting with Ub-containing novel DUB family, functions as a deubiquitylase. It is involved in multiple biological processes and associated with various signaling pathways, playing a significant role in maintaining normal cellular functions and disease-related processes. Genetic models, such as KO/CKO mouse models, can be valuable for studying its functions.
In breast cancer, MINDY1 interacts with estrogen receptor α (ERα), mediating ERα deubiquitination and increasing its stability, promoting breast cancer cell proliferation. High MINDY1 expression is associated with poor prognosis [1].
In bladder cancer, MINDY1 interacts with, deubiquitylates, and stabilizes YAP, promoting bladder cancer progression [2].
In liver cancer, MINDY1 is highly expressed in liver cancer stem cells. Knocking out MINDY1 reduces the expression of stem markers, self-renewal ability, and tumor growth, and its high expression is an independent risk factor for poor prognosis [3].
In conclusion, MINDY1 is crucial in regulating the stability of key proteins in multiple cancer-related signaling pathways. The study of MINDY1 using KO/CKO mouse models has provided insights into its role in breast, bladder, and liver cancers, offering potential therapeutic targets for these diseases.
References：
1. Tang, Jianing, Luo, Yongwen, Long, Guo, Zhou, Ledu. 2021. MINDY1 promotes breast cancer cell proliferation by stabilizing estrogen receptor α. In Cell death & disease, 12, 937. doi:10.1038/s41419-021-04244-z. https://pubmed.ncbi.nlm.nih.gov/34645792/
2. Luo, Yongwen, Zhou, Jun, Tang, Jianing, Liu, Tongzu, Liu, Tao. 2021. MINDY1 promotes bladder cancer progression by stabilizing YAP. In Cancer cell international, 21, 395. doi:10.1186/s12935-021-02095-4. https://pubmed.ncbi.nlm.nih.gov/34315490/
3. Xia, B L, Liu, K W, Huang, H X, Wang, B, Gao, J. . [Deubiquitinating enzyme MINDY1 is an independent risk factor for the maintenance of stemness and poor prognosis in liver cancer cells]. In Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology, 31, 518-523. doi:10.3760/cma.j.cn501113-20230130-00028. https://pubmed.ncbi.nlm.nih.gov/37365029/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen