C57BL/6JCya-Sf3a3em1flox/Cya
Common Name:
Sf3a3-flox
Product ID:
S-CKO-16152
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Sf3a3-flox
Strain ID
CKOCMP-75062-Sf3a3-B6J-VA
Gene Name
Product ID
S-CKO-16152
Gene Alias
4930512K19Rik; 60kDa
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sf3a3em1flox/Cya mice (Catalog S-CKO-16152) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000030734
NCBI RefSeq
NM_029157
Target Region
Exon 7
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
SF3A3, a component of the spliceosome, is involved in the RNA splicing process, a central RNA-based process commonly altered in human cancers. It plays a role in ensuring accurate splicing of mRNAs, especially those enriched for mitochondrial regulators [4]. SF3A3 is also associated with multiple biological processes such as cell cycle regulation, apoptosis, and tumorigenesis [3].
In various cancers, abnormal expression of SF3A3 has been observed. In bladder cancer, its up-regulation is correlated with poor prognosis, and overexpression promotes while depletion reduces the growth of cancer cells [1]. In non-small cell lung cancer, circSCAP binds to SF3A3, facilitating its degradation and activating the p53 signaling pathway to inhibit malignancy [2]. In acute promyelocytic leukemia, knockdown of SF3A3 suppresses the growth of NB4 cells and causes G1/S cell cycle arrest [3]. In breast cancer, its protein levels predict aggressive features, and in hepatocellular carcinoma, the STIL/FOXM1 axis activates SF3A3 to expedite cancer development [4,5].
In conclusion, SF3A3 is crucial in the spliceosome-mediated RNA splicing process. Its dysregulation significantly impacts multiple cancer-related biological processes such as cell growth, apoptosis, and cell cycle progression in various cancers including bladder, lung, leukemia, breast, and liver cancers. Research on SF3A3 provides potential therapeutic targets for these cancer types.
References:
1. Liu, Kai-Long, Yin, Yue-Wei, Lu, Bao-Sai, Yang, Zhan, Li, Wei. 2022. E2F6/KDM5C promotes SF3A3 expression and bladder cancer progression through a specific hypomethylated DNA promoter. In Cancer cell international, 22, 109. doi:10.1186/s12935-022-02475-4. https://pubmed.ncbi.nlm.nih.gov/35248043/
2. Chen, Dongni, Zhou, Hongli, Cai, Zhuochen, Wang, Hui-Yun, Wen, Zhesheng. 2022. CircSCAP interacts with SF3A3 to inhibit the malignance of non-small cell lung cancer by activating p53 signaling. In Journal of experimental & clinical cancer research : CR, 41, 120. doi:10.1186/s13046-022-02299-0. https://pubmed.ncbi.nlm.nih.gov/35365208/
3. Chang, Jiayin, Yan, Shihai, Geng, Zhirong, Wang, Zhilin. 2023. Inhibition of splicing factors SF3A3 and SRSF5 contributes to As3+/Se4+ combination-mediated proliferation suppression and apoptosis induction in acute promyelocytic leukemia cells. In Archives of biochemistry and biophysics, 743, 109677. doi:10.1016/j.abb.2023.109677. https://pubmed.ncbi.nlm.nih.gov/37356608/
4. Cieśla, Maciej, Ngoc, Phuong Cao Thi, Cordero, Eugenia, Bosch, Ana, Bellodi, Cristian. 2021. Oncogenic translation directs spliceosome dynamics revealing an integral role for SF3A3 in breast cancer. In Molecular cell, 81, 1453-1468.e12. doi:10.1016/j.molcel.2021.01.034. https://pubmed.ncbi.nlm.nih.gov/33662273/
5. Zhang, Haijun, Zhang, Lin, Wu, Ziqi. 2025. Interaction of STIL with FOXM1 regulates SF3A3 transcription in the hepatocellular carcinoma development. In Cell division, 20, 1. doi:10.1186/s13008-025-00142-4. https://pubmed.ncbi.nlm.nih.gov/39825314/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen