C57BL/6JCya-Dusp18em1flox/Cya
Common Name:
Dusp18-flox
Product ID:
S-CKO-16180
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Dusp18-flox
Strain ID
CKOCMP-75219-Dusp18-B6J-VA
Gene Name
Product ID
S-CKO-16180
Gene Alias
4930527G07Rik; DSP18; DUSP20; LMWDSP20
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dusp18em1flox/Cya mice (Catalog S-CKO-16180) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000055931
NCBI RefSeq
NM_173745
Target Region
Exon 2
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Dusp18, a dual-specificity phosphatase, belongs to the protein tyrosine phosphatase family. It can dephosphorylate both phospho-tyrosyl and phospho-seryl/threonyl residues. Dusp18 is involved in multiple biological pathways, such as mitogenic signal transduction, cell cycle control, and various disease-related processes [4,5].
In a mouse model of colorectal cancer, inhibition of Dusp18 impairs cholesterol biosynthesis and promotes anti-tumor immunity. Dusp18 dephosphorylates and stabilizes USF1, which induces SREBF2, allowing cells to accumulate lanosterol and release it into the tumor microenvironment. Lanosterol uptake by CD8+ T cells suppresses their activation, and Dusp18 inhibition can enhance anti-tumor immunity [1].
In osteoclasts, Kisspeptin-10 binding to Gpr54 up-regulates Dusp18, which dephosphorylates Src at Tyr 416. Kiss1, Gpr54, and Dusp18 knockout mice all exhibit osteoclast hyperactivation and bone loss, indicating Dusp18's role in preventing bone loss [2].
In hepatocellular carcinoma cells, hypoxia-induced HIF1A activates Dusp18-mediated MAPK14 dephosphorylation, promoting cell migration and invasion [3].
In conclusion, Dusp18 plays essential roles in multiple biological processes and diseases. Mouse models with Dusp18 knockout or inhibition have revealed its functions in cancer immunity, bone metabolism, and cancer cell migration. Understanding Dusp18's functions through these models provides insights into potential therapeutic strategies for colorectal cancer, bone-loss-related diseases, and hepatocellular carcinoma.
References:
1. Zhou, Xiaojun, Wang, Genxin, Tian, Chenhui, Prochownik, Edward V, Li, Youjun. 2024. Inhibition of DUSP18 impairs cholesterol biosynthesis and promotes anti-tumor immunity in colorectal cancer. In Nature communications, 15, 5851. doi:10.1038/s41467-024-50138-x. https://pubmed.ncbi.nlm.nih.gov/38992029/
2. Li, Zhenxi, Yang, Xinghai, Fu, Ruifeng, Liu, Mingyao, Xiao, Jianru. 2024. Kisspeptin-10 binding to Gpr54 in osteoclasts prevents bone loss by activating Dusp18-mediated dephosphorylation of Src. In Nature communications, 15, 1300. doi:10.1038/s41467-024-44852-9. https://pubmed.ncbi.nlm.nih.gov/38346942/
3. Pu, Jian, Qin, Zebang, Fang, Quan, Zeng, Dongyun, Wei, Huamei. 2022. Hypoxia-induced HIF1A activates DUSP18-mediated MAPK14 dephosphorylation to promote hepatocellular carcinoma cell migration and invasion. In Pathology, research and practice, 237, 153955. doi:10.1016/j.prp.2022.153955. https://pubmed.ncbi.nlm.nih.gov/35841693/
4. Wu, Qihan, Gu, Shaohua, Dai, Jianliang, Xie, Yi, Mao, Yumin. . Molecular cloning and characterization of a novel dual-specificity phosphatase18 gene from human fetal brain. In Biochimica et biophysica acta, 1625, 296-304. doi:. https://pubmed.ncbi.nlm.nih.gov/12591617/
5. Wu, Qihan, Huang, Shengdong, Sun, Yaqiong, Xie, Yi, Mao, Yumin. 2006. Dual specificity phosphotase 18, interacting with SAPK, dephosphorylates SAPK and inhibits SAPK/JNK signal pathway in vivo. In Frontiers in bioscience : a journal and virtual library, 11, 2714-24. doi:. https://pubmed.ncbi.nlm.nih.gov/16720344/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen