C57BL/6JCya-Tomm20lem1flox/Cya
Common Name
Tomm20l-flox
Product ID
S-CKO-16184
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-75266-Tomm20l-B6J-VA
When using this mouse strain in a publication, please cite “Tomm20l-flox Mouse (Catalog S-CKO-16184) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Tomm20l-flox
Strain ID
CKOCMP-75266-Tomm20l-B6J-VA
Gene Name
Product ID
S-CKO-16184
Gene Alias
4930553D19Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 12
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000021482
NCBI RefSeq
NM_029227
Target Region
Exon 3
Size of Effective Region
~1.0 kb
Overview of Gene Research
Tomm20l is a testis-enriched gene. Although its exact essential function, associated pathways, and overall biological importance have not been comprehensively detailed in the given references, gene-knockout (KO) mouse models can be a powerful tool to explore its role in biological processes [1].
In a study using the CRISPR/Cas9 system to generate KO mice for 12 testis-enriched genes including Tomm20l, mating tests revealed that Tomm20l, at least when individually ablated and not in combination with other potentially compensatory paralogous genes, is not essential for male fertility in vivo [1].
In conclusion, through the KO mouse model-based research, Tomm20l has been shown to be dispensable for male fertility. This finding contributes to understanding the genetic basis of male fertility and may prevent redundant efforts in generating KO mice for this gene [1].
References：
1. Oyama, Yuki, Miyata, Haruhiko, Shimada, Keisuke, Matzuk, Martin M, Ikawa, Masahito. . CRISPR/Cas9-mediated genome editing reveals 12 testis-enriched genes dispensable for male fertility in mice. In Asian journal of andrology, 24, 266-272. doi:10.4103/aja.aja_63_21. https://pubmed.ncbi.nlm.nih.gov/34290169/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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