C57BL/6NCya-Zbtb3em1flox/Cya
Common Name:
Zbtb3-flox
Product ID:
S-CKO-16192
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Zbtb3-flox
Strain ID
CKOCMP-75291-Zbtb3-B6N-VA
Gene Name
Product ID
S-CKO-16192
Gene Alias
4930563M09Rik
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Zbtb3em1flox/Cya mice (Catalog S-CKO-16192) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000172175
NCBI RefSeq
NM_001098237
Target Region
Exon 2
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Zbtb3, belonging to the Zinc finger and BTB/POZ domain containing transcription factor family, is involved in multiple biological processes. It plays a role in regulating the ROS detoxification pathway, which is crucial for cancer cell growth [5]. Additionally, it is implicated in chromatin looping-mediated regulation of genes like IRF5, having implications in autoimmune diseases [1,3].
In endometrial cancer, the E3 ubiquitin ligase complex SPOP-CUL3-RBX1 targets ZBTB3 for degradation. EC-associated SPOP mutants are defective in this regulation, leading to ZBTB3 accumulation and promoting endometrial cell proliferation, migration, and invasion. Sonic hedgehog (SHH), a transcriptional target of ZBTB3, is upregulated in this context [2]. In embryonic stem cells (ESCs), lncKdm2b activates Zbtb3, which in turn potentiates ESC self-renewal in a Nanog-dependent manner. Zbtb3 deficiency impairs ESC self-renewal and early embryonic development [4].
In conclusion, Zbtb3 is essential for cancer cell growth through the ROS detoxification system, and is involved in the regulation of endometrial cancer progression and embryonic stem cell self-renewal. The study of Zbtb3 using gene knockout or conditional knockout models in relevant diseases helps to understand its role in these biological processes and offers potential therapeutic targets for cancer and insights into early embryonic development.
References：
1. Wang, Zhao, Liang, Qian, Qian, Xinyi, Song, Weihong, Li, Mulin Jun. 2023. An autoimmune pleiotropic SNP modulates IRF5 alternative promoter usage through ZBTB3-mediated chromatin looping. In Nature communications, 14, 1208. doi:10.1038/s41467-023-36897-z. https://pubmed.ncbi.nlm.nih.gov/36869052/
2. Jin, Xiaofeng, Wang, Jian, Li, Qian, Wang, Chenji, Gao, Kun. 2019. SPOP targets oncogenic protein ZBTB3 for destruction to suppress endometrial cancer. In American journal of cancer research, 9, 2797-2812. doi:. https://pubmed.ncbi.nlm.nih.gov/31911863/
3. Hou, Guojun, Zhou, Tian, Xu, Ning, Ye, Zhizhong, Shen, Nan. 2023. Integrative Functional Genomics Identifies Systemic Lupus Erythematosus Causal Genetic Variant in the IRF5 Risk Locus. In Arthritis & rheumatology (Hoboken, N.J.), 75, 574-585. doi:10.1002/art.42390. https://pubmed.ncbi.nlm.nih.gov/36245280/
4. Ye, Buqing, Liu, Benyu, Yang, Liuliu, Tian, Yong, Fan, Zusen. 2018. LncKdm2b controls self-renewal of embryonic stem cells via activating expression of transcription factor Zbtb3. In The EMBO journal, 37, . doi:10.15252/embj.201797174. https://pubmed.ncbi.nlm.nih.gov/29535137/
5. Lim, Ji-Hong. . Zinc finger and BTB domain-containing protein 3 is essential for the growth of cancer cells. In BMB reports, 47, 405-10. doi:. https://pubmed.ncbi.nlm.nih.gov/24856827/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen