C57BL/6JCya-Dynlrb2em1flox/Cya
Common Name:
Dynlrb2-flox
Product ID:
S-CKO-16231
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Dynlrb2-flox
Strain ID
CKOCMP-75465-Dynlrb2-B6J-VA
Gene Name
Product ID
S-CKO-16231
Gene Alias
1700009A04Rik; DNLC2B; Dncl2b
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dynlrb2em1flox/Cya mice (Catalog S-CKO-16231) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000078170
NCBI RefSeq
NM_029297
Target Region
Exon 3~4
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Dynlrb2, short for dynein light chain roadblock-type 2, is a component of the cytoplasmic dynein complex. It plays crucial roles in intracellular transport, being essential for the retrograde transport of murine leukemia virus (MLV) [3,7]. It also participates in spindle formation during male meiosis I [4]. In addition, its associated lncRNAs, such as DYNLRB2-AS1 and DYNLRB2-2, are involved in various biological processes like gemcitabine resistance in nasopharyngeal carcinoma, cholesterol homeostasis, and macrophage foam cell formation [1,2,5,6,8].
In Dynlrb2 knockout (KO) mouse testes, meiosis progression is arrested in metaphase I due to the formation of multipolar spindles with fragmented pericentriolar material (PCM), highlighting its importance in male meiotic spindle bipolarity [4]. Silencing Dynlrb2 in cells reduces the efficiency of MLV infection, while its overexpression increases infection, indicating its significance in MLV retrograde transport and infection [3,7].
In summary, Dynlrb2 is essential for MLV traffic and nuclear entry, as well as for male meiotic spindle formation. The study of Dynlrb2 KO mouse models has provided valuable insights into its role in these biological processes. Its associated lncRNAs also contribute to various disease-related mechanisms, such as cancer chemoresistance and atherosclerosis development [1-5,8,9].
References：
1. Chen, Kai-Lin, Huang, Sai-Wei, Yao, Ji-Jin, Ma, Jun, Liu, Na. 2024. LncRNA DYNLRB2-AS1 promotes gemcitabine resistance of nasopharyngeal carcinoma by inhibiting the ubiquitination degradation of DHX9 protein. In Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy, 76, 101111. doi:10.1016/j.drup.2024.101111. https://pubmed.ncbi.nlm.nih.gov/38908233/
2. Li, Yongqiang, Sun, Tao, Shen, Shuxin, Wang, Lixia, Yan, Jifeng. 2019. LncRNA DYNLRB2-2 inhibits THP-1 macrophage foam cell formation by enhancing autophagy. In Biological chemistry, 400, 1047-1057. doi:10.1515/hsz-2018-0461. https://pubmed.ncbi.nlm.nih.gov/30903747/
3. Pietrantoni, Gianfranco, Gaete-Argel, Aracelly, Herrera-Rojo, Diego, Valiente-Echeverría, Fernando, Arriagada, Gloria. 2021. Dynein Light-Chain Dynlrb2 Is Essential for Murine Leukemia Virus Traffic and Nuclear Entry. In Journal of virology, 95, e0017021. doi:10.1128/JVI.00170-21. https://pubmed.ncbi.nlm.nih.gov/33980598/
4. He, Shuwen, Gillies, John P, Zang, Juliana L, DeSantis, Morgan E, Shibuya, Hiroki. 2023. Distinct dynein complexes defined by DYNLRB1 and DYNLRB2 regulate mitotic and male meiotic spindle bipolarity. In Nature communications, 14, 1715. doi:10.1038/s41467-023-37370-7. https://pubmed.ncbi.nlm.nih.gov/36973253/
5. Hu, Yan-Wei, Yang, Jun-Yao, Ma, Xin, Zheng, Lei, Wang, Qian. 2014. A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent signal transduction pathway is essential for the regulation of cholesterol homeostasis. In Journal of lipid research, 55, 681-97. doi:10.1194/jlr.M044669. https://pubmed.ncbi.nlm.nih.gov/24493833/
6. Zhang, Shun, Li, Lu, Wang, Jie, Xing, Dongming, Chen, Wujun. 2021. Recent advances in the regulation of ABCA1 and ABCG1 by lncRNAs. In Clinica chimica acta; international journal of clinical chemistry, 516, 100-110. doi:10.1016/j.cca.2021.01.019. https://pubmed.ncbi.nlm.nih.gov/33545111/
7. Opazo, Tatiana, Garcés, Andrea, Tapia, Diego, Cancino, Jorge, Arriagada, Gloria. 2017. Functional Evidence of the Involvement of the Dynein Light Chain DYNLRB2 in Murine Leukemia Virus Infection. In Journal of virology, 91, . doi:10.1128/JVI.00129-17. https://pubmed.ncbi.nlm.nih.gov/28250122/
8. Li, Yongqiang, Shen, Shuxin, Ding, Shoukun, Wang, Lixia. 2017. LincRNA DYN-LRB2-2 upregulates cholesterol efflux by decreasing TLR2 expression in macrophages. In Journal of cellular biochemistry, 119, 1911-1921. doi:10.1002/jcb.26352. https://pubmed.ncbi.nlm.nih.gov/28815701/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen