C57BL/6JCya-Ep400em1flox/Cya
Common Name:
Ep400-flox
Product ID:
S-CKO-16260
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ep400-flox
Strain ID
CKOCMP-75560-Ep400-B6J-VA
Gene Name
Product ID
S-CKO-16260
Gene Alias
1700020J09Rik; mDomino; mKIAA1498; p400
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ep400em1flox/Cya mice (Catalog S-CKO-16260) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000041558
NCBI RefSeq
NM_029337
Target Region
Exon 4~5
Size of Effective Region
~3.6 kb
Detailed Document
Overview of Gene Research
Ep400, also known as E1A binding protein p400, is a core catalytic ATPase subunit of ATP-dependent chromatin remodeling complexes [4]. It plays crucial roles in multiple biological processes such as transcription, DNA repair, cell cycle control, and embryonic development [3]. It is involved in histone acetylation and histone dimer exchange activities as part of the TIP60 complex, and deposits histone variants H2AZ and H3.3 into promoters and enhancers during gene activation, contributing to gene regulation [3,5].
In various in vivo studies, Ep400 has shown significant impacts. In mice, depletion of Ep400 in oocytes led to a decline in oocyte quality, abnormal fertilization, reduced major zygotic genome activation, and developmental arrest at the 2-to-4-cell stage [1]. Ep400-deficient Schwann cells in mice caused peripheral neuropathy due to terminal differentiation defects [2]. In oligodendrocytes, deletion of Ep400 at defined development times impaired terminal differentiation, myelination, and led to increased DNA damage and apoptosis [6]. Also, knockdown of Ep400 ortholog in Drosophila increased seizure susceptibility and abnormal neuronal firing, and ep400-deficient zebrafish showed brain developmental abnormalities, epileptic discharges, etc [4].
In conclusion, Ep400 is essential for many biological processes including early embryo development, cell differentiation in the nervous system, and myelination. The gene-knockout and related in vivo models have revealed its significant roles in conditions like peripheral neuropathy, oocyte-related developmental issues, and epilepsy-associated neurodevelopmental disorders, providing valuable insights into understanding the underlying mechanisms and potential therapeutic targets for these diseases.
References:
1. Tian, Qing, Yin, Ying, Tian, Yu, Xiang, Wenpei, Zhou, Li-Quan. 2024. Chromatin Modifier EP400 Regulates Oocyte Quality and Zygotic Genome Activation in Mice. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2308018. doi:10.1002/advs.202308018. https://pubmed.ncbi.nlm.nih.gov/38493496/
2. Fröb, Franziska, Sock, Elisabeth, Tamm, Ernst R, Fukunaga, Rikiro, Wegner, Michael. 2019. Ep400 deficiency in Schwann cells causes persistent expression of early developmental regulators and peripheral neuropathy. In Nature communications, 10, 2361. doi:10.1038/s41467-019-10287-w. https://pubmed.ncbi.nlm.nih.gov/31142747/
3. Chen, Ke, Wang, Li, Yu, Zishuo, Wang, Qianmin, Xu, Yanhui. 2024. Structure of the human TIP60 complex. In Nature communications, 15, 7092. doi:10.1038/s41467-024-51259-z. https://pubmed.ncbi.nlm.nih.gov/39154037/
4. Luo, Sheng, Wang, Peng-Yu, Zhou, Peng, Meng, Heng, Liao, Wei-Ping. 2024. Variants in EP400, encoding a chromatin remodeler, cause epilepsy with neurodevelopmental disorders. In American journal of human genetics, 112, 87-105. doi:10.1016/j.ajhg.2024.11.010. https://pubmed.ncbi.nlm.nih.gov/39708813/
5. Pradhan, Suman K, Su, Trent, Yen, Linda, Kurdistani, Siavash K, Carey, Michael F. 2015. EP400 Deposits H3.3 into Promoters and Enhancers during Gene Activation. In Molecular cell, 61, 27-38. doi:10.1016/j.molcel.2015.10.039. https://pubmed.ncbi.nlm.nih.gov/26669263/
6. Elsesser, Olga, Fröb, Franziska, Küspert, Melanie, Fukunaga, Rikiro, Wegner, Michael. . Chromatin remodeler Ep400 ensures oligodendrocyte survival and is required for myelination in the vertebrate central nervous system. In Nucleic acids research, 47, 6208-6224. doi:10.1093/nar/gkz376. https://pubmed.ncbi.nlm.nih.gov/31081019/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen