C57BL/6JCya-Tmem14aem1flox/Cya
Common Name:
Tmem14a-flox
Product ID:
S-CKO-16308
Background:
C57BL/6JCya
Product Type
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Basic Information
Strain Name
Tmem14a-flox
Strain ID
CKOCMP-75712-Tmem14a-B6J-VA
Gene Name
Product ID
S-CKO-16308
Gene Alias
5730496E24Rik; PTD011
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tmem14aem1flox/Cya mice (Catalog S-CKO-16308) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027064
NCBI RefSeq
NM_001290679
Target Region
Exon 4
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Tmem14a, a transmembrane protein, has been implicated in multiple biological processes. It contains a putative transmembrane domain and is an integral transmembrane protein of 99 amino acids with three transmembrane domains [3,4]. While its exact function is still being elucidated, studies suggest it may play roles in apoptosis regulation, cell proliferation, and energy metabolism, and is associated with maintaining the integrity of the glomerular filtration barrier [3,4].
In ovarian cancer, Tmem14a is highly expressed and is correlated with prognostic conditions. It inhibits cell apoptosis, accelerates energy metabolism like glycolysis and oxygen respiration, and is positively correlated with c-Myc. Overexpression of c-Myc can rescue its function, indicating its potential as a diagnostic and prognostic biomarker and a molecular target for therapy [1].
In non-small-cell lung cancer (NSCLC), AXL transcriptionally up-regulates Tmem14a expression to mediate cell proliferation. Silencing Tmem14a reduces cellular proliferation and ATP levels, suggesting it could be a therapeutic target for NSCLC patients undergoing AXL-targeted therapies [2].
In human ovarian cancer cells, knockdown of Tmem14a by RNAi reduces cell proliferation, causes cell cycle arrest, suppresses cell invasion, and down-regulates related proteins and the phosphorylation of Smad2 and Smad3 in the TGF-β signalling pathway, indicating a pro-tumorigenic effect [5].
In zebrafish embryos, knocking down tmem14a mRNA translation results in proteinuria, suggesting its role in maintaining glomerular filtration barrier integrity [4].
In conclusion, Tmem14a is involved in various biological processes, especially in cancer-related events such as cell apoptosis, proliferation, and invasion. Its role in maintaining the integrity of the glomerular filtration barrier is also significant. Functional studies, including those using gene knockdown models, have provided insights into its functions in specific disease conditions, highlighting its potential as a biomarker and therapeutic target in ovarian cancer and NSCLC [1,2,4,5].
References：
1. Zhang, Qingmei, Wang, Xiaohong, Zhang, Xuan, Jia, Jin, Chen, Jie. 2022. TMEM14A aggravates the progression of human ovarian cancer cells by enhancing the activity of glycolysis. In Experimental and therapeutic medicine, 24, 614. doi:10.3892/etm.2022.11551. https://pubmed.ncbi.nlm.nih.gov/36160886/
2. Jang, Te-Hsuan, Lin, Sheng-Chieh, Yang, Ya-Yu, Kuo, Tsu-Hsiang, Chuang, Shuang-En. 2023. AXL transcriptionally up-regulates TMEM14A expression to mediate cell proliferation in non-small-cell lung cancer cells. In Biochemical and biophysical research communications, 682, 365-370. doi:10.1016/j.bbrc.2023.10.027. https://pubmed.ncbi.nlm.nih.gov/37839105/
3. Woo, Im Sun, Jin, Hana, Kang, Eun Sil, Choi, Wan Sung, Seo, Han Geuk. 2011. TMEM14A inhibits N-(4-hydroxyphenyl)retinamide-induced apoptosis through the stabilization of mitochondrial membrane potential. In Cancer letters, 309, 190-8. doi:10.1016/j.canlet.2011.05.031. https://pubmed.ncbi.nlm.nih.gov/21723035/
4. Khalil, Ramzi, Bonnemaijer, Josephine D D, Kreutz, Reinhold, Hogendoorn, Pancras C W, Baelde, Hans J. . Transmembrane protein 14A protects glomerular filtration barrier integrity. In Physiological reports, 11, e15847. doi:10.14814/phy2.15847. https://pubmed.ncbi.nlm.nih.gov/38054547/
5. Zhang, Qingmei, Chen, Xiufeng, Zhang, Xuan, Zhan, Jingfen, Chen, Jie. 2016. Knockdown of TMEM14A expression by RNAi inhibits the proliferation and invasion of human ovarian cancer cells. In Bioscience reports, 36, e00298. doi:10.1042/BSR20150258. https://pubmed.ncbi.nlm.nih.gov/26896463/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen