C57BL/6JCya-Idnkem1flox/Cya
Common Name:
Idnk-flox
Product ID:
S-CKO-16315
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Idnk-flox
Strain ID
CKOCMP-75731-Idnk-B6J-VA
Gene Name
Product ID
S-CKO-16315
Gene Alias
5133401N09Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Idnkem1flox/Cya mice (Catalog S-CKO-16315) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000051490
NCBI RefSeq
NM_198004
Target Region
Exon 2~3
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Idnk, also known as gluconokinase, is involved in the catabolism of L-idonic acid in Escherichia coli. It encodes a thermosensitive gluconate kinase that catalyzes an ATP-dependent phosphorylation of D-gluconate to form 6-phosphogluconate, which is further metabolized via the Entner-Doudoroff pathway. The idnK gene is monocistronic and transcribed divergently from the idnD-idnO-idnT-idnR operon. The genes of this L-idonate pathway are under catabolite repression control [2,3].
In hepatocellular carcinoma (HCC) cells, knockdown of IDNK significantly inhibited cell proliferation and increased cell apoptosis. Microarray analysis of BEL-7404 cells transfected with IDNK shRNA lentivirus plasmid identified 1196 differentially expressed genes, and the pathway of tRNA charging was significantly inhibited. This indicates that IDNK may be a candidate therapeutic target for HCC [1].
In conclusion, Idnk has an important function in the L-idonic acid catabolic pathway in E. coli. In the context of HCC, IDNK plays a key role in cell proliferation and apoptosis, and its knockdown-based studies suggest its potential as a therapeutic target for this deadly cancer.
References：
1. Wu, Xiao-Min, Jin, Cheng, Gu, Yuan-Long, Zhang, Shuo, Zhang, Zhen. 2020. Gluconokinase IDNK Promotes Cell Proliferation and Inhibits Apoptosis in Hepatocellular Carcinoma. In OncoTargets and therapy, 13, 1767-1776. doi:10.2147/OTT.S234055. https://pubmed.ncbi.nlm.nih.gov/32161472/
2. Bausch, Christoph, Ramsey, Matthew, Conway, Tyrrell. . Transcriptional organization and regulation of the L-idonic acid pathway (GntII system) in Escherichia coli. In Journal of bacteriology, 186, 1388-97. doi:. https://pubmed.ncbi.nlm.nih.gov/14973046/
3. Bausch, C, Peekhaus, N, Utz, C, Lowary, T, Conway, T. . Sequence analysis of the GntII (subsidiary) system for gluconate metabolism reveals a novel pathway for L-idonic acid catabolism in Escherichia coli. In Journal of bacteriology, 180, 3704-10. doi:. https://pubmed.ncbi.nlm.nih.gov/9658018/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen