C57BL/6JCya-Smurf1em1flox/Cya
Common Name:
Smurf1-flox
Product ID:
S-CKO-16342
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Smurf1-flox
Strain ID
CKOCMP-75788-Smurf1-B6J-VA
Gene Name
Product ID
S-CKO-16342
Gene Alias
4930431E10Rik; mKIAA1625
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Smurf1em1flox/Cya mice (Catalog S-CKO-16342) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000085684
NCBI RefSeq
NM_001038627
Target Region
Exon 2~3
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Smurf1, also known as SMAD specific E3 ubiquitin protein ligase 1, is a HECT-type E3 ubiquitin ligase. It plays crucial roles in multiple biological pathways, such as the bone morphogenetic protein pathway, non-canonical Wnt pathway, and mitogen-activated protein kinase pathway [7]. It is involved in cell growth, morphogenesis, migration, polarity, and autophagy, and is related to physiological manifestations like age-dependent bone formation deficiency and tumor cell invasion [7]. Genetic models, like Smurf1-knockout mice, have been valuable in studying its functions.
In cancer, overexpression of Smurf1 is linked to poor prognosis in various cancers. For example, in glioblastoma, colon cancer, and clear cell renal cell carcinoma, it acts as a tumor promoter by ubiquitinating and degrading tumor-suppressing proteins [1]. In KRAS-mutated colorectal cancer, Smurf1-mediated PDK1 neddylation is essential for PI3K-Akt signaling and tumorigenesis, and Smurf1 deficiency reduces tumorigenesis in a genetic mouse model [3]. In hepatocellular carcinoma, Smurf1-mediated ubiquitination of UVRAG promotes autophagosome maturation and inhibits tumor growth [2]. In glioblastoma, ER stress induces Smurf1 degradation, and knockdown of Smurf1 leads to cell apoptosis, while overexpression activates NRF2 signaling to protect cells from ER-stress-induced death [4].
In lupus nephritis, SMURF1 upregulation activates the cGAS/STING/IFN-1 signal axis by ubiquitinating YY1, facilitating apoptosis in podocytes, and SMURF1 knockdown inhibits disease progression in vivo [6]. In dry age-related macular degeneration, inhibiting Smurf1 alleviates acute retina injury, and Smurf1 is involved in exacerbating oxidative stress injury and inflammation in the retina [8]. In ameloblasts, Smurf1 regulates cell polarization through ubiquitination-mediated degradation of RhoA, and local knockdown in rat lower incisor ameloblasts results in enamel matrix secretion disorder [5].
In conclusion, Smurf1 is a key regulator in multiple biological processes and disease conditions. Model-based research, especially using Smurf1 KO/CKO mouse models, has revealed its significance in cancer, lupus nephritis, age-related macular degeneration, and tooth development. Understanding Smurf1 provides insights into disease mechanisms and potential therapeutic targets.
References:
1. Xia, Qin, Li, Yang, Han, Da, Dong, Lei. 2020. SMURF1, a promoter of tumor cell progression? In Cancer gene therapy, 28, 551-565. doi:10.1038/s41417-020-00255-8. https://pubmed.ncbi.nlm.nih.gov/33204002/
2. Feng, Xing, Jia, Yanyan, Zhang, Yuyu, Qiu, Xingfeng, Zhang, Zhiyong. 2019. Ubiquitination of UVRAG by SMURF1 promotes autophagosome maturation and inhibits hepatocellular carcinoma growth. In Autophagy, 15, 1130-1149. doi:10.1080/15548627.2019.1570063. https://pubmed.ncbi.nlm.nih.gov/30686098/
3. Peng, Zhiqiang, Fang, Wei, Wu, Bo, Cui, Chun-Ping, Zhang, Lingqiang. 2024. Targeting Smurf1 to block PDK1-Akt signaling in KRAS-mutated colorectal cancer. In Nature chemical biology, 21, 59-70. doi:10.1038/s41589-024-01683-5. https://pubmed.ncbi.nlm.nih.gov/39039255/
4. Dong, Lei, Xu, Mengchuan, Li, Yang, Zhou, Liying, Xia, Qin. 2023. SMURF1 attenuates endoplasmic reticulum stress by promoting the degradation of KEAP1 to activate NRF2 antioxidant pathway. In Cell death & disease, 14, 361. doi:10.1038/s41419-023-05873-2. https://pubmed.ncbi.nlm.nih.gov/37316499/
5. Niu, Haoman, Bi, Fei, Zhao, Wenjun, Guo, Weihua, Chen, Yu. 2022. Smurf1 regulates ameloblast polarization by ubiquitination-mediated degradation of RhoA. In Cell proliferation, 56, e13387. doi:10.1111/cpr.13387. https://pubmed.ncbi.nlm.nih.gov/36579844/
6. Li, Xiaoyan, Tao, Sisi, Xu, Zhiquan, Xiang, Wei, He, Xiaojie. 2023. SMURF1 activates the cGAS/STING/IFN-1 signal axis by mediating YY1 ubiquitination to accelerate the progression of lupus nephritis. In Autoimmunity, 56, 2281235. doi:10.1080/08916934.2023.2281235. https://pubmed.ncbi.nlm.nih.gov/37994046/
7. Cao, Yu, Zhang, Lingqiang. 2012. A Smurf1 tale: function and regulation of an ubiquitin ligase in multiple cellular networks. In Cellular and molecular life sciences : CMLS, 70, 2305-17. doi:10.1007/s00018-012-1170-7. https://pubmed.ncbi.nlm.nih.gov/23007848/
8. Li, Duo, Wei, Ting-Ting, Cai, Jiping, Yao, Yong, Zhu, Lingpeng. 2023. Smurf1: A possible therapeutic target in dry age-related macular degeneration. In Experimental eye research, 233, 109549. doi:10.1016/j.exer.2023.109549. https://pubmed.ncbi.nlm.nih.gov/37348673/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen