C57BL/6JCya-Senp2em1flox/Cya
Common Name:
Senp2-flox
Product ID:
S-CKO-16351
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Senp2-flox
Strain ID
CKOCMP-75826-Senp2-B6J-VA
Gene Name
Product ID
S-CKO-16351
Gene Alias
2310007L05Rik; 4930538C18Rik; Smt3ip2; SuPr-1; mKIAA1331
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Senp2em1flox/Cya mice (Catalog S-CKO-16351) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000023561
NCBI RefSeq
NM_029457
Target Region
Exon 4
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Senp2, short for SUMO-specific protease 2, is crucial in regulating the SUMOylation-de-SUMOylation balance. SUMOylation is a post-translational modification that impacts protein function, localization, and stability. Senp2's function is to remove SUMO (small ubiquitin-like modifier) from substrate proteins, thus affecting various biological pathways. It is involved in processes such as energy metabolism, cell proliferation, and calcium homeostasis, making it biologically significant in multiple tissues and disease contexts. Genetic models, like knockout (KO) or conditional knockout (CKO) mouse models, have been valuable in understanding its functions.
In pancreatic β cells, Senp2-βKO mice had impaired glucose tolerance and insulin secretion, along with mitochondrial dysfunction, suggesting Senp2 regulates mitochondrial function and insulin secretion by modulating DRP1 phosphorylation [2]. In adipocytes, Senp2-aKO mice were resistant to diet-induced obesity due to increased energy expenditure, as Senp2 negatively regulates browning of white adipose tissue by de-conjugating SUMO from C/EBPβ [1]. In brown adipose tissue development, SENP2-mediated de-SUMOylation of CREB suppresses Necdin expression, promoting brown adipocyte differentiation, shown through the analysis of a SENP2 BAT conditional knockout mouse model [4]. In Th17 cells, deleting Senp2 in T-cell lineages exacerbated experimental colitis, revealing its role in restraining Th17-mediated inflammation [3].
In conclusion, Senp2 plays essential roles in energy metabolism, including insulin secretion and adipose tissue browning, as well as in cell-specific functions like Th17-mediated inflammation. The KO/CKO mouse models have been instrumental in uncovering these functions, which are relevant to diseases such as diabetes, obesity, and inflammatory bowel diseases. Understanding Senp2's functions provides insights into potential therapeutic targets for these disease areas.
References:
1. Lee, Ji Seon, Chae, Sehyun, Nan, Jinyan, Chung, Sung Soo, Park, Kyong Soo. . SENP2 suppresses browning of white adipose tissues by de-conjugating SUMO from C/EBPβ. In Cell reports, 38, 110408. doi:10.1016/j.celrep.2022.110408. https://pubmed.ncbi.nlm.nih.gov/35196497/
2. Nan, Jinyan, Lee, Ji Seon, Moon, Joon Ho, Chung, Sung Soo, Park, Kyong Soo. 2022. SENP2 regulates mitochondrial function and insulin secretion in pancreatic β cells. In Experimental & molecular medicine, 54, 72-80. doi:10.1038/s12276-021-00723-7. https://pubmed.ncbi.nlm.nih.gov/35064188/
3. Yang, Tsan-Tzu, Chiang, Ming-Feng, Chang, Che-Chang, Hsu, Wei, Lin, Kuo-I. 2023. SENP2 restrains the generation of pathogenic Th17 cells in mouse models of colitis. In Communications biology, 6, 629. doi:10.1038/s42003-023-05009-4. https://pubmed.ncbi.nlm.nih.gov/37301920/
4. Liang, Qiuli, Zheng, Quan, Zuo, Yong, Ni, Peihua, Cheng, Jinke. . SENP2 Suppresses Necdin Expression to Promote Brown Adipocyte Differentiation. In Cell reports, 28, 2004-2011.e4. doi:10.1016/j.celrep.2019.07.083. https://pubmed.ncbi.nlm.nih.gov/31433978/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen