C57BL/6JCya-Gstk1em1flox/Cya
Common Name:
Gstk1-flox
Product ID:
S-CKO-16425
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Gstk1-flox
Strain ID
CKOCMP-76263-Gstk1-B6J-VA
Gene Name
Product ID
S-CKO-16425
Gene Alias
0610025I19Rik; DsbA-L
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gstk1em1flox/Cya mice (Catalog S-CKO-16425) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031897
NCBI RefSeq
NM_029555
Target Region
Exon 2~3
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Gstk1, also known as glutathione S-transferase kappa 1, is a key enzyme involved in multiple biological processes. It is crucial for maintaining peroxisomal redox homeostasis, as it contains GSH-dependent disulfide bond oxidoreductase activity [1]. It is also involved in energy production, lipid metabolism, and is a key regulator for adiponectin secretion and multimerization [3,5]. It may play a role in the regulation of insulin resistance and related metabolic diseases [3].
In mouse models of hypertrophic cardiomyopathy (HCM), Gstk1 was significantly downregulated across five different etiology models. Knocking out gstk1 in zebrafish increased the expression of sarcomere genes and decreased end-diastolic volume, suggesting that Gstk1 downregulation may be a common mechanism underlying HCM, potentially through increasing oxidative stress [2]. In Gstk1-deficient mice, no general underlying oxidative stress was found, but glomerular basement membrane changes and microalbuminuria were observed, indicating a role in glomerular nephropathy development [4].
In conclusion, Gstk1 is essential for maintaining redox balance, energy and lipid metabolism, and adiponectin-related processes. Model-based research, especially gene-knockout models in mice and zebrafish, has revealed its significant roles in diseases such as hypertrophic cardiomyopathy and glomerular nephropathy, providing insights into disease mechanisms and potential therapeutic targets.
References:
1. Costa, Cláudio F, Lismont, Celien, Chornyi, Serhii, Waterham, Hans R, Fransen, Marc. 2023. Functional Analysis of GSTK1 in Peroxisomal Redox Homeostasis in HEK-293 Cells. In Antioxidants (Basel, Switzerland), 12, . doi:10.3390/antiox12061236. https://pubmed.ncbi.nlm.nih.gov/37371965/
2. Sasagawa, Shota, Nishimura, Yuhei, Okabe, Shiko, Ito, Masaaki, Tanaka, Toshio. 2016. Downregulation of GSTK1 Is a Common Mechanism Underlying Hypertrophic Cardiomyopathy. In Frontiers in pharmacology, 7, 162. doi:10.3389/fphar.2016.00162. https://pubmed.ncbi.nlm.nih.gov/27378925/
3. Hu, Chun, Sun, Lin. . [Advance in glutathione S-transferase kappa 1]. In Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences, 42, 468-475. doi:10.11817/j.issn.1672-7347.2017.04.017. https://pubmed.ncbi.nlm.nih.gov/28490708/
4. Blackburn, Anneke C, Coggan, Marjorie, Shield, Alison J, Matthaei, Klaus I, Board, Philip G. 2011. Glutathione transferase kappa deficiency causes glomerular nephropathy without overt oxidative stress. In Laboratory investigation; a journal of technical methods and pathology, 91, 1572-83. doi:10.1038/labinvest.2011.107. https://pubmed.ncbi.nlm.nih.gov/21826057/
5. Morel, Fabrice, Aninat, Caroline. 2011. The glutathione transferase kappa family. In Drug metabolism reviews, 43, 281-91. doi:10.3109/03602532.2011.556122. https://pubmed.ncbi.nlm.nih.gov/21428694/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen