C57BL/6JCya-Fads1em1flox/Cya
Common Name:
Fads1-flox
Product ID:
S-CKO-16427
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Fads1-flox
Strain ID
CKOCMP-76267-Fads1-B6J-VA
Gene Name
Product ID
S-CKO-16427
Gene Alias
0710001O03Rik; A930006B21Rik; DSD
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fads1em1flox/Cya mice (Catalog S-CKO-16427) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000010807
NCBI RefSeq
NM_146094
Target Region
Exon 1~2
Size of Effective Region
~3.4 kb
Detailed Document
Overview of Gene Research
Fads1, also known as Delta-5 desaturase, is a rate-limiting enzyme in long-chain polyunsaturated fatty acid (LCPUFA) synthesis [1,3]. It is involved in the biosynthesis of arachidonic acid (AA), an essential polyunsaturated fatty acid. Fads1-associated pathways include the conversion of AA to prostaglandin E2 (PGE2) via the TLR4/MYD88 pathway in colorectal cancer cells, and it also plays a role in lipid metabolism, which is related to various biological processes and diseases [1].
In colorectal cancer, AA feeding promotes tumor growth in AOM/DSS and intestinal-specific Apc-/-mice. Fads1 is upregulated in CRC, and it effectively mediates AA synthesis. Functionally, Fads1 regulates CRC tumor growth through a high AA microenvironment-induced enrichment of gram-negative microbes. Eliminating gram-negative microbes abolishes the Fads1 effect [1].
In triple-negative breast cancer, Fads1/2 are highly expressed in a subset of TNBC with a poorer prognosis. Targeting Fads1/2 by genetic interference and pharmacological approaches renders tumors ferroptosis-resistant [2].
In hepatocyte-specific models, AAV8-mediated overexpression of Fads1 in rats fed a high-fat high-fructose diet reduced plasma triglyceride and hepatic cholesterol levels, while in low-fat high-fructose diet-fed rats, it improved glucose tolerance and insulin signaling [3].
In conclusion, Fads1 is crucial for LCPUFA synthesis and lipid metabolism. Its dysregulation is associated with various diseases such as colorectal cancer, triple-negative breast cancer, and metabolic syndrome-related conditions. Mouse models, including those with altered Fads1 expression, have revealed its role in tumor growth regulation, ferroptosis susceptibility, and metabolic phenotype modulation, providing insights into disease mechanisms and potential therapeutic targets.
References:
1. Xu, Chunjie, Gu, Lei, Hu, Lipeng, Zhang, Xueli, Xu, Qing. 2023. FADS1-arachidonic acid axis enhances arachidonic acid metabolism by altering intestinal microecology in colorectal cancer. In Nature communications, 14, 2042. doi:10.1038/s41467-023-37590-x. https://pubmed.ncbi.nlm.nih.gov/37041160/
2. Lorito, Nicla, Subbiani, Angela, Smiriglia, Alfredo, Bachi, Angela, Morandi, Andrea. 2024. FADS1/2 control lipid metabolism and ferroptosis susceptibility in triple-negative breast cancer. In EMBO molecular medicine, 16, 1533-1559. doi:10.1038/s44321-024-00090-6. https://pubmed.ncbi.nlm.nih.gov/38926633/
3. Ghooray, Dushan T, Xu, Manman, Shi, Hongxue, McClain, Craig J, Song, Ming. 2024. Hepatocyte-Specific Fads1 Overexpression Attenuates Western Diet-Induced Metabolic Phenotypes in a Rat Model. In International journal of molecular sciences, 25, . doi:10.3390/ijms25094836. https://pubmed.ncbi.nlm.nih.gov/38732052/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen