C57BL/6JCya-Slc24a2em1flox/Cya
Common Name:
Slc24a2-flox
Product ID:
S-CKO-16448
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Slc24a2-flox
Strain ID
CKOCMP-76376-Slc24a2-B6J-VA
Gene Name
Product ID
S-CKO-16448
Gene Alias
2810021B17Rik; 6330417K15Rik; Nckx2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc24a2em1flox/Cya mice (Catalog S-CKO-16448) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000107158
NCBI RefSeq
NM_001110240
Target Region
Exon 4
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Slc24a2, also known as solute carrier 24 family member 2, encodes a potassium-dependent sodium-calcium exchanger (NCKX2) that can transport metal ions across the cell membrane [2]. It is involved in maintaining intracellular calcium homeostasis, which is critical for many physiological functions [4].
In the context of diseases, SLC24A2 has been implicated in multiple conditions. In a study on type 2 diabetes (T2D) and cancer, SLC24A2 was identified as a key gene strongly associated with fasting plasma glucose. Overlapped differentially expressed genes between T2D and control, and SLC24A2-high and SLC24A2-low were analyzed, and it was found that SLC24A2 may link T2D and cancer by influencing the ribosome function of islet β cells [1]. In pancreatic ductal adenocarcinoma, a new nonsynonymous single nucleotide variant in SLC24A2 was found, which decreased the stability of the SLC24A2 protein [2].
In neuropathic pain, the expression of SLC24A2 and its encoded protein NCKX2 were downregulated in the posterior horn of the spinal cord in a sciatic nerve chronic constriction injury model. Overexpression of SLC24A2 reduced hyperalgesia and decreased inflammatory cytokines, and miR-135a-5p was shown to regulate SLC24A2 [4]. Additionally, SLC24A2 was among the genes whose expression was affected by valproic acid exposure in human forebrain organoids, and these genes were significantly overlapped with those in autism-spectrum-disorder-related pathways [5].
In colon adenocarcinoma, a prognostic model based on calcium extrusion-related genes including SLC24A2 was constructed, and high-risk scores of these genes were associated with poorer prognosis [6]. Also, SLC24A2 has been associated with skin color variation in Chinese populations [3], and genes near SLC24A2 were associated with the correlation structure of CVD risk factors in a Ghanaian population [7].
In conclusion, Slc24a2 plays essential roles in maintaining intracellular calcium homeostasis and is associated with multiple diseases such as T2D, cancer, neuropathic pain, autism-spectrum disorder, and CVD. Studies on Slc24a2, especially those using in-vivo models like the ones described above, help to understand its biological functions and its implications in disease mechanisms, potentially providing new insights for disease treatment and prevention.
References：
1. Bian, Qin, Li, Haijun, Wang, Xiaoyi, Liang, Tingting, Zhang, Kai. 2022. Multiomics Integrated Analysis Identifies SLC24A2 as a Potential Link between Type 2 Diabetes and Cancer. In Journal of diabetes research, 2022, 4629419. doi:10.1155/2022/4629419. https://pubmed.ncbi.nlm.nih.gov/35601016/
2. Wang, Lei, Shao, Zhuo, Chen, Shiyue, Shi, Lu, Li, Zhaoshen. . A SLC24A2 Gene Variant Uncovered in Pancreatic Ductal Adenocarcinoma by Whole Exome Sequencing. In The Tohoku journal of experimental medicine, 241, 287-295. doi:10.1620/tjem.241.287. https://pubmed.ncbi.nlm.nih.gov/28413183/
3. Wang, Fudi, Luo, Qi, Chen, Yan, Liu, Fan, Wang, Sijia. 2021. A Genome-Wide Scan on Individual Typology Angle Found Variants at SLC24A2 Associated with Skin Color Variation in Chinese Populations. In The Journal of investigative dermatology, 142, 1223-1227.e14. doi:10.1016/j.jid.2021.07.186. https://pubmed.ncbi.nlm.nih.gov/34570997/
4. Zhou, Xiao-Gang, He, Hui, Wang, Pei-Ji. 2020. A critical role for miR‑135a‑5p‑mediated regulation of SLC24A2 in neuropathic pain. In Molecular medicine reports, 22, 2115-2122. doi:10.3892/mmr.2020.11262. https://pubmed.ncbi.nlm.nih.gov/32582988/
5. Meng, Qingtuan, Zhang, Wendiao, Wang, Xuan, Tang, Beisha, Chen, Chao. 2022. Human forebrain organoids reveal connections between valproic acid exposure and autism risk. In Translational psychiatry, 12, 130. doi:10.1038/s41398-022-01898-x. https://pubmed.ncbi.nlm.nih.gov/35351869/
6. Jin, Mingpeng, Yin, Chun, Yang, Jie, Zhu, Jianjun, Yuan, Jian. 2024. Identification and validation of calcium extrusion-related genes prognostic signature in colon adenocarcinoma. In PeerJ, 12, e17582. doi:10.7717/peerj.17582. https://pubmed.ncbi.nlm.nih.gov/39006025/
7. Kodaman, Nuri, Sobota, Rafal S, Asselbergs, Folkert W, Aldrich, Melinda C, Williams, Scott M. 2017. Genetic Effects on the Correlation Structure of CVD Risk Factors: Exome-Wide Data From a Ghanaian Population. In Global heart, 12, 133-140. doi:10.1016/j.gheart.2017.01.013. https://pubmed.ncbi.nlm.nih.gov/28408189/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen