C57BL/6JCya-Fbxo31em1flox/Cya
Common Name:
Fbxo31-flox
Product ID:
S-CKO-16471
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Fbxo31-flox
Strain ID
CKOCMP-76454-Fbxo31-B6J-VA
Gene Name
Product ID
S-CKO-16471
Gene Alias
1110003O08Rik; 2310046N15Rik; Fbx14; Fbxo14
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fbxo31em1flox/Cya mice (Catalog S-CKO-16471) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000059018
NCBI RefSeq
NM_133765
Target Region
Exon 7
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Fbxo31, a member of the F-box family that comprises the SCF complex, is involved in regulating protein ubiquitination and degradation, which is crucial for various biological processes such as cell growth, migration, and invasion [1-7]. It participates in multiple pathways, like the METTL3-FBXO31-SIRT2 axis in pancreatic cancer, the FBXO31-GPX4 interaction in cholangiocarcinoma, and the c-Myc-FBXO31 feedback regulation in ovarian cancer [1,2,4]. Genetic models are valuable for studying its functions.
In pancreatic cancer, FBXO31 is overexpressed, promoting tumor growth, migration, and invasion by regulating SIRT2 ubiquitination and degradation. METTL3 up-regulates FBXO31 expression through m6A modification [1].
In cholangiocarcinoma, FBXO31 is downregulated, and its deficiency is associated with the TNM stage. Overexpression of FBXO31 inhibits cell growth, migration, and cancer stem cell properties, and sensitizes cancer stem-like cells to cisplatin by promoting ferroptosis and GPX4 degradation [2].
In glioma, low FBXO31 expression indicates poor prognosis, and its overexpression suppresses lipogenesis and tumor progression by promoting CD147 ubiquitination and degradation [3].
In ovarian cancer, c-Myc suppresses FBXO31 mRNA levels, while FBXO31 facilitates c-Myc polyubiquitination and degradation, inhibiting ovarian cancer growth [4].
In prostate cancer, low FBXO31 mRNA levels are associated with high pre-operative prostate-specific antigen levels and Gleason grade. FBXO31 promotes DUSP6 degradation, and its depletion dysregulates ERK and PI3K-AKT signaling, promoting tumor development [5].
In esophageal squamous cell carcinoma, high FBXO31 expression is associated with poor prognosis and Taxol chemoresistance. Silencing FBXO31 sensitizes cells to Taxol, and this effect is associated with cofilin-1 [6].
In liver fibrosis, FBXO31 is upregulated, and it modulates hepatic stellate cell activation and liver fibrogenesis by promoting Smad7 ubiquitination [7].
In conclusion, FBXO31 plays diverse roles in regulating biological processes and is involved in multiple disease conditions such as various cancers, premature ovarian insufficiency, and liver fibrosis. Functional studies, especially those using knockout or conditional knockout mouse models (implied by in vivo experiments in references), have revealed its significance in tumorigenesis, cell-specific functions, and disease-associated pathways, providing potential therapeutic targets for these diseases.
References：
1. Chen, Kai, Wang, Yue, Dai, Xingna, Wang, Zhiwei, Ma, Jia. 2024. FBXO31 is upregulated by METTL3 to promote pancreatic cancer progression via regulating SIRT2 ubiquitination and degradation. In Cell death & disease, 15, 37. doi:10.1038/s41419-024-06425-y. https://pubmed.ncbi.nlm.nih.gov/38216561/
2. Zhu, Zhiwen, Zheng, Yang, He, Huijuan, Dai, Wei, Huang, Haili. 2022. FBXO31 sensitizes cancer stem cells-like cells to cisplatin by promoting ferroptosis and facilitating proteasomal degradation of GPX4 in cholangiocarcinoma. In Liver international : official journal of the International Association for the Study of the Liver, 42, 2871-2888. doi:10.1111/liv.15462. https://pubmed.ncbi.nlm.nih.gov/36269678/
3. Feng, Yan, Liu, Mingli, Xie, Peng, Dong, Ruifeng, Hao, Zhongfei. 2022. FBXO31 suppresses lipogenesis and tumor progression in glioma by promoting ubiquitination and degradation of CD147. In Prostaglandins & other lipid mediators, 163, 106667. doi:10.1016/j.prostaglandins.2022.106667. https://pubmed.ncbi.nlm.nih.gov/35940557/
4. Islam, Sehbanul, Dutta, Parul, Sahay, Osheen, Shetty, Praveenkumar, Santra, Manas Kumar. 2022. Feedback-regulated transcriptional repression of FBXO31 by c-Myc triggers ovarian cancer tumorigenesis. In International journal of cancer, 150, 1512-1524. doi:10.1002/ijc.33854. https://pubmed.ncbi.nlm.nih.gov/34706096/
5. Duan, Shanshan, Moro, Loredana, Qu, Rui, Arbini, Arnaldo A, Pagano, Michele. . Loss of FBXO31-mediated degradation of DUSP6 dysregulates ERK and PI3K-AKT signaling and promotes prostate tumorigenesis. In Cell reports, 37, 109870. doi:10.1016/j.celrep.2021.109870. https://pubmed.ncbi.nlm.nih.gov/34686346/
6. Lv, Liang, Wang, Shu Chao, Mo, Jin You, Xu, Mei Li, Liu, Jia. 2021. Effects and mechanisms of FBXO31 on Taxol chemoresistance in esophageal squamous cell carcinoma. In Biochemical and biophysical research communications, 586, 129-136. doi:10.1016/j.bbrc.2021.11.082. https://pubmed.ncbi.nlm.nih.gov/34839191/
7. He, Huijuan, Dai, Jialiang, Feng, Jialing, Xu, Aizhong, Huang, Haili. 2019. FBXO31 modulates activation of hepatic stellate cells and liver fibrogenesis by promoting ubiquitination of Smad7. In Journal of cellular biochemistry, 121, 3711-3719. doi:10.1002/jcb.29528. https://pubmed.ncbi.nlm.nih.gov/31680332/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen