C57BL/6JCya-Mfsd2aem1flox/Cya
Common Name:
Mfsd2a-flox
Product ID:
S-CKO-16507
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mfsd2a-flox
Strain ID
CKOCMP-76574-Mfsd2a-B6J-VA
Gene Name
Product ID
S-CKO-16507
Gene Alias
1700018O18Rik; Mfsd2; NLS1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mfsd2aem1flox/Cya mice (Catalog S-CKO-16507) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000030408
NCBI RefSeq
NM_029662
Target Region
Exon 3
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Mfsd2a, a member of the major facilitator superfamily, is a sodium-dependent lysophosphatidylcholine (LPC) transporter. It is crucial for normal brain growth and function as it is the major transporter for the essential omega-3 fatty acid docosahexaenoic acid (DHA) uptake into the brain. Mfsd2a is expressed in the endothelium of the blood-brain barrier micro-vessels and plays a role in maintaining the integrity of the blood-brain barrier, which is essential for providing a proper environment for neural function [1,4].
In Mfsd2a-knockout mice, there is a marked reduction in brain DHA levels, accompanied by neuronal cell loss in the hippocampus and cerebellum, cognitive deficits, severe anxiety, and microcephaly [1]. These mice also have a leaky blood-brain barrier from embryonic stages to adulthood due to increased CNS-endothelial-cell vesicular transcytosis, despite normal vascular network patterning [4]. In the context of acute kidney injury, Mfsd2a haploinsufficient mice show delayed renal function recovery, and treatment with LPC-DHA can improve the situation [5]. In gastric cancer, higher MFSD2A expression in tumor tissues is associated with a better response to anti-PD-1 immunotherapy, and its overexpression in GC cells enhances the efficacy of anti-PD-1 immunotherapy by reprogramming the tumor microenvironment [2]. In glioma, inhibiting Wnt signaling increases temozolomide (TMZ) delivery by downregulating Mfsd2a and enhancing vascular transcytosis [3].
In conclusion, Mfsd2a is essential for the transport of DHA into the brain and for maintaining the integrity of the blood-brain barrier. Its deficiency in mouse models reveals its significance in brain development, cognitive function, and in diseases such as acute kidney injury, gastric cancer, and glioma. These model-based studies help us understand the role of Mfsd2a in biological processes and disease conditions, providing potential targets for therapeutic intervention.
References:
1. Nguyen, Long N, Ma, Dongliang, Shui, Guanghou, Goh, Eyleen L K, Silver, David L. 2014. Mfsd2a is a transporter for the essential omega-3 fatty acid docosahexaenoic acid. In Nature, 509, 503-6. doi:10.1038/nature13241. https://pubmed.ncbi.nlm.nih.gov/24828044/
2. Zhang, Bin, Wang, Chun-Mei, Wu, Hao-Xiang, Xu, Rui-Hua, Cao, Xue-Tao. 2023. MFSD2A potentiates gastric cancer response to anti-PD-1 immunotherapy by reprogramming the tumor microenvironment to activate T cell response. In Cancer communications (London, England), 43, 1097-1116. doi:10.1002/cac2.12476. https://pubmed.ncbi.nlm.nih.gov/37539769/
3. Xie, Yuan, He, Liqun, Zhang, Yanyu, Wang, Liang, Zhang, Lei. . Wnt signaling regulates MFSD2A-dependent drug delivery through endothelial transcytosis in glioma. In Neuro-oncology, 25, 1073-1084. doi:10.1093/neuonc/noac288. https://pubmed.ncbi.nlm.nih.gov/36591963/
4. Ben-Zvi, Ayal, Lacoste, Baptiste, Kur, Esther, Yan, Han, Gu, Chenghua. 2014. Mfsd2a is critical for the formation and function of the blood-brain barrier. In Nature, 509, 507-11. doi:10.1038/nature13324. https://pubmed.ncbi.nlm.nih.gov/24828040/
5. Loke, Randy Y J, Chin, Cheen Fei, Liang, Gao, Torta, Federico, Silver, David L. 2023. Mfsd2a-mediated lysolipid transport is important for renal recovery after acute kidney injury. In Journal of lipid research, 64, 100416. doi:10.1016/j.jlr.2023.100416. https://pubmed.ncbi.nlm.nih.gov/37467896/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen