C57BL/6JCya-Upp2em1flox/Cya
Common Name:
Upp2-flox
Product ID:
S-CKO-16528
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Upp2-flox
Strain ID
CKOCMP-76654-Upp2-B6J-VA
Gene Name
Product ID
S-CKO-16528
Gene Alias
1700124F02Rik; UDRPASE2; UP2; UPASE2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Upp2em1flox/Cya mice (Catalog S-CKO-16528) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000059102
NCBI RefSeq
NM_001289659
Target Region
Exon 3
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Upp2, also known as uridine phosphorylase 2, is an enzyme that catalyzes the reversible conversion of uridine to uracil and ribose-1-phosphate. It is involved in the pyrimidine salvage pathway, which plays a critical role in nucleotide metabolism. This pathway is essential for cells to recycle nucleotides and maintain the balance of nucleotide pools, which is crucial for DNA and RNA synthesis, energy metabolism, and cell survival [4].
In a study on nutrient-limited conditions, it was found that the catabolism of uridine, where UPP1/UPP2 phosphorylytically cleaves uridine into uracil and ribose-1-phosphate, can support glycolysis. The ribose moiety from uridine or RNA can be salvaged to meet energy requirements via the pentose phosphate pathway and glycolysis [1]. Another study showed that in mice, overexpression of hepatic MIC19 led to increased UPP2 activity, causing uracil accumulation in the liver, which was associated with increased energy expenditure and pedestrian locomotion [2]. In the context of circadian rhythms and hepatocellular carcinoma, Upp2 was identified as a BMAL1-regulated gene. Overexpression of human UPP2 protein inhibited the proliferation and migration of HepG2 cells, suggesting its potential role in HCC prevention and treatment [3].
In conclusion, Upp2 is crucial in nucleotide metabolism and has far-reaching impacts on energy metabolism, locomotion, and potentially in cancer-related processes. Studies using mouse models have revealed its role in these biological functions and disease-related conditions, providing valuable insights into the underlying mechanisms and potential therapeutic targets.
References:
1. Skinner, Owen S, Blanco-Fernández, Joan, Goodman, Russell P, Mootha, Vamsi K, Jourdain, Alexis A. 2023. Salvage of ribose from uridine or RNA supports glycolysis in nutrient-limited conditions. In Nature metabolism, 5, 765-776. doi:10.1038/s42255-023-00774-2. https://pubmed.ncbi.nlm.nih.gov/37198474/
2. Sohn, Jee Hyung, Mutlu, Beste, Latorre-Muro, Pedro, Banks, Alexander S, Puigserver, Pere. 2023. Liver mitochondrial cristae organizing protein MIC19 promotes energy expenditure and pedestrian locomotion by altering nucleotide metabolism. In Cell metabolism, 35, 1356-1372.e5. doi:10.1016/j.cmet.2023.06.015. https://pubmed.ncbi.nlm.nih.gov/37473754/
3. Zhao, Hongcong, Han, Guohao, Jiang, Zhou, Jin, Yaping, Chen, Huatao. 2023. Identification of BMAL1-Regulated circadian genes in mouse liver and their potential association with hepatocellular carcinoma: Gys2 and Upp2 as promising candidates. In Biochemical and biophysical research communications, 696, 149422. doi:10.1016/j.bbrc.2023.149422. https://pubmed.ncbi.nlm.nih.gov/38183795/
4. Roosild, Tarmo P, Castronovo, Samantha, Villoso, Adelbert, Ziemba, Amy, Pizzorno, Giuseppe. 2011. A novel structural mechanism for redox regulation of uridine phosphorylase 2 activity. In Journal of structural biology, 176, 229-37. doi:10.1016/j.jsb.2011.08.002. https://pubmed.ncbi.nlm.nih.gov/21855639/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen