C57BL/6JCya-Arpc2em1flox/Cya
Common Name:
Arpc2-flox
Product ID:
S-CKO-16537
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Arpc2-flox
Strain ID
CKOCMP-76709-Arpc2-B6J-VA
Gene Name
Product ID
S-CKO-16537
Gene Alias
2210023N03Rik; 34kDa; p34-Arc
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Arpc2em1flox/Cya mice (Catalog S-CKO-16537) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000006467
NCBI RefSeq
NM_029711
Target Region
Exon 5
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Arpc2, or Actin-related protein 2/3 complex subunit 2, is a crucial actin-binding component involved in the regulation of actin polymerization. It mediates the formation of branched actin networks, which is fundamental for maintaining the dynamics of the F-actin cytoskeleton, a key factor in many biological processes like cell migration, invasion, and intercellular force maintenance. It is associated with pathways such as the TGF-β pathway and is important for epithelial-mesenchymal transition (EMT) [1,2].
In cancer research, studies on human cell lines and animal models have been pivotal. In breast cancer, siRNA-ARPC2 transfection showed that ARPC2 promotes cell proliferation, migration, and invasion, and activates the TGF-β pathway to contribute to EMT [1]. In hepatocellular carcinoma (HCC), silencing ARPC2 in HCC cells significantly inhibited cell proliferation, migration, and invasion, while overexpression promoted these processes [2]. In gastric cancer, ARPC2 promoted cell proliferation and invasion in the MKN-28 cell line, and was highly expressed in cancer tissues compared to normal ones, being associated with poor prognosis [4]. Benproperine, an ARPC2 inhibitor, suppressed cancer cell migration and tumor metastasis in animal models, suggesting ARPC2 as a potential anti-metastatic target [3].
In conclusion, Arpc2 plays a significant role in processes related to cell migration, invasion, and proliferation, especially in cancer development. Research on Arpc2 using gene-editing techniques in cell lines and animal models has provided insights into its role in breast, liver, and gastric cancers, among others. These findings suggest that Arpc2 could be a potential therapeutic target for anti-cancer treatment.
References:
1. Cheng, Zhongle, Wei, Wei, Wu, Zhengshen, Han, Yinli, Wu, Qiang. 2019. ARPC2 promotes breast cancer proliferation and metastasis. In Oncology reports, 41, 3189-3200. doi:10.3892/or.2019.7113. https://pubmed.ncbi.nlm.nih.gov/31002363/
2. Huang, Shenglan, Dong, Cairong, Li, Dan, Xu, Yongkang, Wu, Jianbing. 2022. ARPC2: A Pan-Cancer Prognostic and Immunological Biomarker That Promotes Hepatocellular Carcinoma Cell Proliferation and Invasion. In Frontiers in cell and developmental biology, 10, 896080. doi:10.3389/fcell.2022.896080. https://pubmed.ncbi.nlm.nih.gov/35733852/
3. Yoon, Yae Jin, Han, Young-Min, Choi, Jiyeon, Han, Dong Cho, Kwon, Byoung-Mog. 2019. Benproperine, an ARPC2 inhibitor, suppresses cancer cell migration and tumor metastasis. In Biochemical pharmacology, 163, 46-59. doi:10.1016/j.bcp.2019.01.017. https://pubmed.ncbi.nlm.nih.gov/30710516/
4. Zhang, Jun, Liu, Yi, Yu, Chang-Jun, Ding, Rui, Wang, Hong. 2017. Role of ARPC2 in Human Gastric Cancer. In Mediators of inflammation, 2017, 5432818. doi:10.1155/2017/5432818. https://pubmed.ncbi.nlm.nih.gov/28694563/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen