C57BL/6JCya-Snx27em1flox/Cya
Common Name:
Snx27-flox
Product ID:
S-CKO-16543
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Snx27-flox
Strain ID
CKOCMP-76742-Snx27-B6J-VA
Gene Name
Product ID
S-CKO-16543
Gene Alias
5730552M22Rik; ESTM45; ESTM47
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Snx27em1flox/Cya mice (Catalog S-CKO-16543) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000107283
NCBI RefSeq
NM_001082484
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Snx27 belongs to the sorting nexin (SNX) family of proteins. It is unique in containing both a Phox (PX) domain and an additional PDZ domain. Snx27 plays a critical role in protein sorting and trafficking in the endocytosis pathway, mediating the recycling of internalized proteins from endosomes to the plasma membrane in a PDZ domain-dependent manner. It is associated with the retromer complex and is involved in multiple cellular functions, which are essential for normal cellular function and preventing human diseases [1,3].
In T-cell activation, Snx27 translocates to the T cell-APC interface upon TCR activation, facilitating the transport of cargoes and interacting with DGKζ to sustain DAG modulation for IS organization and signaling [2]. In viral infections, Snx27 together with the retromer complex prevents the ACE2/virus complex from entering lysosome/late endosome, decreasing SARS-CoV-2 viral entry [4]. In cytomegalovirus replication, Snx27:Retromer:ESCPE-1-mediated tubulation is essential for the establishment of a Rab10-decorated subset of membranes within the pre-AC, and suppression of Snx27-mediated functions leads to a decrease in infectious virion release [7]. In neurological function, Snx27-mediated recycling of neuroligin-2 regulates inhibitory signaling by enhancing synaptic neuroligin-2 clusters [8]. In cancer, while its role in tumorigenesis is not fully understood, its over-expression is linked to tumorigenesis, cancer progression, and metastasis. For example, circ_Snx27 in hepatocellular carcinoma promotes cancer development via the miR-637/FGFR1 axis and circ-Snx27 sponging miR-375/RPN1 axis contributes to HCC progression [5,6]. Also, Snx27 inhibits TNFα-induced NF-κB signalling activation by facilitating OTULIN to localize to TNF receptor complex [9].
In conclusion, Snx27 is crucial for endosomal protein recycling and has diverse functions in various biological processes and diseases. Its role in T-cell activation, viral infections, cytomegalovirus replication, neurological function, and cancer development has been revealed through different studies. Understanding Snx27's functions provides insights into normal cellular function and offers potential strategies for treating related diseases.
References：
1. Deb, Shreya, Sun, Jun. 2022. Endosomal Sorting Protein SNX27 and Its Emerging Roles in Human Cancers. In Cancers, 15, . doi:10.3390/cancers15010070. https://pubmed.ncbi.nlm.nih.gov/36612066/
2. González-Mancha, Natalia, Mérida, Isabel. 2020. Interplay Between SNX27 and DAG Metabolism in the Control of Trafficking and Signaling at the IS. In International journal of molecular sciences, 21, . doi:10.3390/ijms21124254. https://pubmed.ncbi.nlm.nih.gov/32549284/
3. Chandra, Mintu, Kendall, Amy K, Jackson, Lauren P. 2021. Toward Understanding the Molecular Role of SNX27/Retromer in Human Health and Disease. In Frontiers in cell and developmental biology, 9, 642378. doi:10.3389/fcell.2021.642378. https://pubmed.ncbi.nlm.nih.gov/33937239/
4. Yang, Bo, Jia, Yuanyuan, Meng, Yumin, Qi, Jianxun, Shang, Guijun. . SNX27 suppresses SARS-CoV-2 infection by inhibiting viral lysosome/late endosome entry. In Proceedings of the National Academy of Sciences of the United States of America, 119, . doi:10.1073/pnas.2117576119. https://pubmed.ncbi.nlm.nih.gov/35022217/
5. Li, Hua, Liu, Bingli, Xu, Xin, Zhang, Di, Liu, Qingfeng. 2022. Circ_SNX27 regulates hepatocellular carcinoma development via miR-637/FGFR1 axis. In Environmental toxicology, 37, 2832-2843. doi:10.1002/tox.23640. https://pubmed.ncbi.nlm.nih.gov/36029209/
6. Zheng, Chao, Liang, Jin, Yu, Shoude, Dai, Lin, Xu, Dan. . Circ-SNX27 sponging miR-375/RPN1 axis contributes to hepatocellular carcinoma progression. In The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology, 27, 333-344. doi:10.4196/kjpp.2023.27.4.333. https://pubmed.ncbi.nlm.nih.gov/37386831/
7. Štimac, Igor, Marcelić, Marina, Radić, Barbara, Lučin, Pero, Mahmutefendić Lučin, Hana. 2024. SNX27:Retromer:ESCPE-1-mediated early endosomal tubulation impacts cytomegalovirus replication. In Frontiers in cellular and infection microbiology, 14, 1399761. doi:10.3389/fcimb.2024.1399761. https://pubmed.ncbi.nlm.nih.gov/39359939/
8. Halff, Els F, Szulc, Blanka R, Lesept, Flavie, Kittler, Josef T. . SNX27-Mediated Recycling of Neuroligin-2 Regulates Inhibitory Signaling. In Cell reports, 29, 2599-2607.e6. doi:10.1016/j.celrep.2019.10.096. https://pubmed.ncbi.nlm.nih.gov/31775031/
9. Shi, Ruona, Shi, Xue, Qin, Dajiang, Vermeulen, Michiel, Zhang, Xiaofei. 2021. SNX27-driven membrane localisation of OTULIN antagonises linear ubiquitination and NF-κB signalling activation. In Cell & bioscience, 11, 146. doi:10.1186/s13578-021-00659-5. https://pubmed.ncbi.nlm.nih.gov/34315543/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen