C57BL/6JCya-Nkrfem1flox/Cya
Common Name:
Nkrf-flox
Product ID:
S-CKO-16662
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Nkrf-flox
Strain ID
CKOCMP-77286-Nkrf-B6J-VA
Gene Name
Product ID
S-CKO-16662
Gene Alias
9430034D17Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nkrfem1flox/Cya mice (Catalog S-CKO-16662) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000239259
NCBI RefSeq
NM_029891
Target Region
Exon 2
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
NKRF, also known as NF-κB-repressing factor, plays a significant role in the transcriptional inhibition of various specific genes and is involved in regulating the NF-κB signaling pathway [1,2,3,4,5,6,7]. The NF-κB signaling pathway is crucial in many biological processes such as inflammation, immune response, and cell survival.
In a CF-specific NKRF-knockout (NKRF-CKO) mouse model, NKRF-CKO mice demonstrated worse cardiac function, reduced survival, increased infarct size, heart weight, and MMP2 and MMP9 expression post-myocardial infarction compared with littermates. NKRF inhibits CF migration and invasion in vitro by down-regulating MMP2 and MMP9 expression. Mechanistically, NKRF inhibits human antigen R (HuR) transcription, decreasing HuR-targeted Mmp2 and Mmp9 mRNA stability [1].
In conclusion, NKRF is essential in the context of cardiac remodeling post-myocardial infarction, as revealed by the NKRF-CKO mouse model. It also has implications in other diseases such as Alzheimer's disease, atherosclerosis, and lung cancer, where it regulates the NF-κB signaling pathway [2,3,6]. Understanding NKRF's function provides potential therapeutic targets for these disease conditions.
References：
1. Guo, Chenghu, Ji, Wei, Yang, Wei, Zhang, Meng, Zhang, Cheng. 2023. NKRF in Cardiac Fibroblasts Protects against Cardiac Remodeling Post-Myocardial Infarction via Human Antigen R. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2303283. doi:10.1002/advs.202303283. https://pubmed.ncbi.nlm.nih.gov/37667861/
2. Zhang, Di, Chen, Keyan, Shan, Li Shen. . Meta-analysis and transcriptomic analysis reveal that NKRF and ZBTB17 regulate the NF-κB signaling pathway, contributing to the shared molecular mechanisms of Alzheimer's disease and atherosclerosis. In CNS neuroscience & therapeutics, 30, e14683. doi:10.1111/cns.14683. https://pubmed.ncbi.nlm.nih.gov/38738952/
3. Li, Yang, Zu, Lingling, Wu, Heng, Guo, Fengjie, Zhou, Qinghua. 2021. MiR-192/NKRF axis confers lung cancer cell chemoresistance to cisplatin via the NF-κB pathway. In Thoracic cancer, 13, 430-441. doi:10.1111/1759-7714.14278. https://pubmed.ncbi.nlm.nih.gov/34953057/
4. Wang, Wen-Fang, Zhong, Hui-Juan, Cheng, Shu, Xiong, Jie, Zhao, Wei-Li. 2023. A nuclear NKRF interacting long noncoding RNA controls EBV eradication and suppresses tumor progression in natural killer/T-cell lymphoma. In Biochimica et biophysica acta. Molecular basis of disease, 1869, 166722. doi:10.1016/j.bbadis.2023.166722. https://pubmed.ncbi.nlm.nih.gov/37084822/
5. Wang, Hongjia, Lian, Xin, Wang, Kexin, Wang, Shuye. 2023. WWP2 binds to NKRF, enhances the NF-κB signaling, and promotes malignant phenotypes of acute myeloid leukemia cells. In Biochemistry and cell biology = Biochimie et biologie cellulaire, 102, 85-95. doi:10.1139/bcb-2022-0360. https://pubmed.ncbi.nlm.nih.gov/37921219/
6. Lu, Jingjing, Shi, Yan, Zhang, Feng, Zhao, Shiqiao, Zhao, Liming. 2023. Mechanism of lnRNA-ICL involved in lung cancer development in COPD patients through modulating microRNA-19-3p/NKRF/NF-κB axis. In Cancer cell international, 23, 58. doi:10.1186/s12935-023-02900-2. https://pubmed.ncbi.nlm.nih.gov/37013587/
7. Hazra, Bibhabasu, Chakraborty, Surajit, Bhaskar, Meenakshi, Mahadevan, Anita, Basu, Anirban. 2019. miR-301a Regulates Inflammatory Response to Japanese Encephalitis Virus Infection via Suppression of NKRF Activity. In Journal of immunology (Baltimore, Md. : 1950), 203, 2222-2238. doi:10.4049/jimmunol.1900003. https://pubmed.ncbi.nlm.nih.gov/31527198/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen