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C57BL/6JCya-Aglem1flox/Cya
Common Name:
Agl-flox
Product ID:
S-CKO-16684
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Agl-flox
Strain ID
CKOCMP-77559-Agl-B6J-VA
Gene Name
Agl
Product ID
S-CKO-16684
Gene Alias
1110061O17Rik; 9430004C13Rik; 9630046L06Rik
Background
C57BL/6JCya
NCBI ID
77559
Modification
Conditional knockout
Chromosome
3
Phenotype
MGI:1924809
Document
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Application
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Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Aglem1flox/Cya mice (Catalog S-CKO-16684) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000040603
NCBI RefSeq
NM_001081326
Target Region
Exon 13~17
Size of Effective Region
~3.5 kb
Detailed Document
Click here to download >>
Overview of Gene Research
AGL, also known as amylo-α-1,6-glucosidase,4-α-glucanotransferase, is an enzyme mainly responsible for glycogen debranching. Mutations in the AGL gene can lead to glycogen storage disease type III (GSDIII), indicating its crucial role in glycogen metabolism [1,2,4].

Germline Agl knockout (Agl-/-) and urothelium-specific conditional Agl knockout (Aglcko) mouse models have been developed. Agl-/-mice exposed to N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) had a higher bladder cancer (BC) incidence compared with wild-type mice. Similarly, Aglcko mice had a higher BC incidence than control (Aglfl/fl) mice. RNA sequencing of tumors from Agl-/-mice identified 19 differentially expressed genes. An 'Agl Loss' gene signature was developed and could stratify normal and tumor samples in BC patient datasets. These suggest that AGL loss promotes carcinogenesis [3]. Also, in bladder cancer, low AGL expression was found to enhance tumor growth by increasing hyaluronic acid synthase 2 (HAS2)-driven hyaluronic acid (HA) synthesis [5].

In conclusion, AGL is essential for glycogen debranching and normal glycogen metabolism. Mouse models with Agl knockout or conditional knockout have revealed its role in promoting bladder carcinogenesis, providing a basis for evaluating Agl expression levels in populations at risk of BC. This research helps in understanding the biological functions of AGL and its implications in disease, especially in bladder cancer.

References:
1. Zhang, Meng, Wang, Chang, Xie, Zhen, Wu, Cheng, Long, Yun. . [Analysis of two cases of glycogen storage disease type III due to compound heterozygous variants of AGL gene]. In Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics, 38, 1073-1076. doi:10.3760/cma.j.cn511374-20200803-00578. https://pubmed.ncbi.nlm.nih.gov/34729746/
2. Shindo, Akito, Ueda, Kazutaka, Minatsuki, Shun, Akazawa, Hiroshi, Komuro, Issei. 2023. Novel AGL variants in a patient with glycogen storage disease type IIIb and pulmonary hypertension caused by pulmonary veno-occlusive disease: A case report. In Frontiers in genetics, 14, 1148067. doi:10.3389/fgene.2023.1148067. https://pubmed.ncbi.nlm.nih.gov/37035733/
3. Sottnik, Joseph L, Mallaredy, Vandana, Chauca-Diaz, Ana, Clouthier, David, Theodorescu, Dan. . Elucidating the role of Agl in bladder carcinogenesis by generation and characterization of genetically engineered mice. In Carcinogenesis, 40, 194-201. doi:10.1093/carcin/bgy139. https://pubmed.ncbi.nlm.nih.gov/30403777/
4. Wang, Jing, Yu, Yuping, Cai, Chunquan, Zhao, Yu, Shu, Jianbo. 2022. The biallelic novel pathogenic variants in AGL gene in a chinese patient with glycogen storage disease type III. In BMC pediatrics, 22, 284. doi:10.1186/s12887-022-03252-y. https://pubmed.ncbi.nlm.nih.gov/35578201/
5. Guin, Sunny, Ru, Yuanbin, Agarwal, Neeraj, Comi, Giacomo P, Theodorescu, Dan. 2015. Loss of Glycogen Debranching Enzyme AGL Drives Bladder Tumor Growth via Induction of Hyaluronic Acid Synthesis. In Clinical cancer research : an official journal of the American Association for Cancer Research, 22, 1274-83. doi:10.1158/1078-0432.CCR-15-1706. https://pubmed.ncbi.nlm.nih.gov/26490312/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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