C57BL/6JCya-Steep1em1flox/Cya
Common Name:
Steep1-flox
Product ID:
S-CKO-16708
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Steep1-flox
Strain ID
CKOCMP-77644-Steep1-B6J-VA
Gene Name
Product ID
S-CKO-16708
Gene Alias
C330007P06Rik; STEEP
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Steep1em1flox/Cya mice (Catalog S-CKO-16708) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000115248
NCBI RefSeq
NM_029951
Target Region
Exon 4
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Steep1, a gene with its function yet to be fully elaborated, was identified as a divergent paralog within suborder Yangochiroptera in the study of positively selected genes in the hoary bat (Lasiurus cinereus) lineage [2]. While its exact function in this context remains unclear, the finding of its divergence in this lineage may suggest its role in the adaptive evolution of these bats, potentially related to the immune and metabolic functions that were prominent among the positively selected genes in this study.
In relation to its potential function, in a different context, STEEP (a related entity, potentially related to Steep1) was found to be a positive regulator of STING signaling. STEEP associated with STING and promoted its trafficking from the endoplasmic reticulum (ER) to the ERGIC/Golgi, where STING signaling occurs. This was achieved through stimulation of phosphatidylinositol-3-phosphate (PtdIns(3)P) production and ER membrane curvature formation, inducing COPII-mediated ER-to-Golgi trafficking of STING. Depletion of STEEP impaired STING-driven gene expression in response to virus infection in brain tissue and in cells from patients with STING-associated diseases [1].
In conclusion, although Steep1's function needs further exploration, the discovery of its divergence in bats may hint at its role in adaptive evolution. Also, the related STEEP's role in STING signaling provides a possible functional framework for understanding Steep1, potentially in relation to immune-related processes and diseases associated with STING dysregulation.
References:
1. Zhang, Bao-Cun, Nandakumar, Ramya, Reinert, Line S, Thomsen, Martin K, Paludan, Søren R. 2020. STEEP mediates STING ER exit and activation of signaling. In Nature immunology, 21, 868-879. doi:10.1038/s41590-020-0730-5. https://pubmed.ncbi.nlm.nih.gov/32690950/
2. Cornman, Robert S, Cryan, Paul M. 2022. Positively selected genes in the hoary bat (Lasiurus cinereus) lineage: prominence of thymus expression, immune and metabolic function, and regions of ancient synteny. In PeerJ, 10, e13130. doi:10.7717/peerj.13130. https://pubmed.ncbi.nlm.nih.gov/35317076/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen