C57BL/6JCya-Cpt1cem1flox/Cya
Common Name:
Cpt1c-flox
Product ID:
S-CKO-16761
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Cpt1c-flox
Strain ID
CKOCMP-78070-Cpt1c-B6J-VA
Gene Name
Product ID
S-CKO-16761
Gene Alias
6530437J22Rik; 9630004I06Rik; CPT I-C; CPT IC; CPT1-B; CPTI-B
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cpt1cem1flox/Cya mice (Catalog S-CKO-16761) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000063761
NCBI RefSeq
NM_153679
Target Region
Exon 4~6
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Cpt1c, a unique isoform of carnitine palmitoyltransferase, is pivotal in regulating fatty acid oxidation. It is involved in lipid metabolic reprogramming and cell adaptation to environmental stressors, rather than being a direct fat burner [2]. Cpt1c is expressed in various tissues, including neurons and is associated with multiple biological processes and diseases.
In gastric cancer, Cpt1c-positive cancer-associated fibroblasts (Cpt1c + CAFs) foster an immunosuppressive tumor microenvironment by promoting the IL-6-induced M2-like phenotype of macrophages, and are associated with therapeutic resistance [1]. In breast cancer, Cpt1c downregulation causes plasma membrane remodelling and anthracycline resistance [3]. In colorectal cancer, Cpt1c-mediated fatty acid oxidation facilitates cell proliferation and metastasis, and its overexpression indicates poor relapse-free survival [4]. In pancreatic cancer, the YY1-Cpt1c signaling axis modulates cell proliferation and metabolism under hypoxia [5]. In esophageal squamous cell carcinoma, Cpt1c promotes tumor progression by upregulating energy supply and accelerating the G1/S transition [6]. In the hypothalamus, Cpt1c in the ventromedial nucleus is necessary for brown fat thermogenesis activation in obesity [7].
In conclusion, Cpt1c plays diverse and significant roles in multiple biological processes and disease conditions, especially in cancer progression, energy homeostasis, and immune regulation. Studies on Cpt1c using gene knockout models in mice have provided valuable insights into its functions in these areas, helping to better understand the underlying mechanisms and potentially guiding the development of therapeutic strategies.
References:
1. Wei, Rongyuan, Song, Junquan, Pan, Hongda, Liu, Xiaowen, Gao, Jianpeng. 2024. CPT1C-positive cancer-associated fibroblast facilitates immunosuppression through promoting IL-6-induced M2-like phenotype of macrophage. In Oncoimmunology, 13, 2352179. doi:10.1080/2162402X.2024.2352179. https://pubmed.ncbi.nlm.nih.gov/38746869/
2. Fadó, Rut, Zagmutt, Sebastian, Herrero, Laura, Serra, Dolors, Casals, Núria. 2023. To be or not to be a fat burner, that is the question for cpt1c in cancer cells. In Cell death & disease, 14, 57. doi:10.1038/s41419-023-05599-1. https://pubmed.ncbi.nlm.nih.gov/36693836/
3. Muley, Helena, Valencia, Karmele, Casas, Josefina, Fadó, Rut, Casals, Núria. 2023. Cpt1c Downregulation Causes Plasma Membrane Remodelling and Anthracycline Resistance in Breast Cancer. In International journal of molecular sciences, 24, . doi:10.3390/ijms24020946. https://pubmed.ncbi.nlm.nih.gov/36674468/
4. Li, Jing, Zheng, Wanwei, Wu, Jie, Huang, Tiansheng, Luo, Zhongguang. . CPT1C-mediated fatty acid oxidation facilitates colorectal cancer cell proliferation and metastasis. In Acta biochimica et biophysica Sinica, 55, 1301-1309. doi:10.3724/abbs.2023041. https://pubmed.ncbi.nlm.nih.gov/37078750/
5. Zhou, Yanying, Chen, Yixin, Zhao, Pengfei, Huang, Min, Bi, Huichang. 2024. The YY1-CPT1C signaling axis modulates the proliferation and metabolism of pancreatic tumor cells under hypoxia. In Biochemical pharmacology, 227, 116422. doi:10.1016/j.bcp.2024.116422. https://pubmed.ncbi.nlm.nih.gov/38996932/
6. Zhao, Huihui, Cheng, Xinxin, Yan, Liping, Wu, Qingnan, Zhan, Qimin. 2024. APC/C-regulated CPT1C promotes tumor progression by upregulating the energy supply and accelerating the G1/S transition. In Cell communication and signaling : CCS, 22, 283. doi:10.1186/s12964-024-01657-z. https://pubmed.ncbi.nlm.nih.gov/38783346/
7. Rodríguez-Rodríguez, Rosalía, Miralpeix, Cristina, Fosch, Anna, Serra, Dolors, Casals, Núria. 2018. CPT1C in the ventromedial nucleus of the hypothalamus is necessary for brown fat thermogenesis activation in obesity. In Molecular metabolism, 19, 75-85. doi:10.1016/j.molmet.2018.10.010. https://pubmed.ncbi.nlm.nih.gov/30448371/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen