C57BL/6JCya-Rassf10em1flox/Cya
Common Name:
Rassf10-flox
Product ID:
S-CKO-16847
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rassf10-flox
Strain ID
CKOCMP-78748-Rassf10-B6J-VA
Gene Name
Product ID
S-CKO-16847
Gene Alias
4632411J06Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rassf10em1flox/Cya mice (Catalog S-CKO-16847) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000182858
NCBI RefSeq
NM_175279
Target Region
Exon 1
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Rassf10, a member of the Ras-association domain family, is a tumor suppressor gene [1-10]. It contains a RA (Ras-association) and two coiled domains, and is involved in multiple biological processes, such as regulating cell cycle, apoptosis, and cell invasion. It is associated with pathways like Wnt/β-catenin, PI3K-AKT, MAPK, and calcium signaling [2-4, 7].
In mouse models, Rassf10 haploinsufficiency promotes neoplasia formation. Rassf10-/-and p53-deficient mice show a three-fold increased rate of kidney cysts compared with p53-/-controls, indicating its role in kidney-related diseases [2]. In various cancers, loss-of-function studies have shown significant effects. For example, in esophageal squamous cell carcinoma, its overexpression reduces cell growth, colony formation, migration, and invasion, and mediates inactivation of the Wnt/β-catenin pathway [1]. In lung cancer cell lines, its epigenetic inactivation is common, and restoring its expression induces apoptosis [3].
In conclusion, Rassf10 functions as a tumor suppressor. Gene knockout mouse models have been crucial in revealing its role in promoting neoplasia in kidney-related diseases and its significance in multiple cancer types, including lung, esophageal, and colorectal cancers. Understanding Rassf10 can provide new insights into cancer development mechanisms and potential therapeutic targets.
References:
1. Liu, Yifei, Zhu, Xiaohui, Zhang, Wenwen, Feng, Jia, Shi, Jiahai. 2022. RASSF10 exhibits tumor‑suppressing potential involving tumor proliferation, metastasis and epithelial‑mesenchymal transition in esophageal squamous cell carcinoma. In Oncology reports, 47, . doi:10.3892/or.2022.8291. https://pubmed.ncbi.nlm.nih.gov/35211758/
2. Richter, Antje M, Woods, Michelle L, Küster, Miriam M, Boettger, Thomas, Dammann, Reinhard H. 2020. RASSF10 is frequently epigenetically inactivated in kidney cancer and its knockout promotes neoplasia in cancer prone mice. In Oncogene, 39, 3114-3127. doi:10.1038/s41388-020-1195-6. https://pubmed.ncbi.nlm.nih.gov/32047266/
3. Wang, Yanfu, Ma, Tianxiao, Bi, Jing, Zhang, Caihua, Gao, Mingyang. 2013. RASSF10 is epigenetically inactivated and induces apoptosis in lung cancer cell lines. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 68, 321-6. doi:10.1016/j.biopha.2013.12.005. https://pubmed.ncbi.nlm.nih.gov/24433832/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen